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Synthesis and biological evaluation ...

Kawamoto, Yusuke.

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Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeres

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeres/ by Yusuke Kawamoto.
作者:	Kawamoto, Yusuke.
出版者:	Singapore :Springer Singapore : : 2019.,
面頁冊數:	xii, 122 p. :ill., digital ;24 cm.
內容註:	1. General Introduction Sequence-specific DNA Binding Pyrrole-Imidazole Polyamides and Their Applications -- 2. Development of a New Method for Synthesis of Tandem Hairpin Pyrrole-Imidazole Polyamide Probes Targeting Human Telomeres -- 3. Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences -- 4. Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole?Imidazole Polyamide Probes.
Contained By:	Springer Nature eBook
標題:	Pyrroles. -
電子資源:	https://doi.org/10.1007/978-981-13-6912-4
ISBN:	9789811369124

Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeres
Kawamoto, Yusuke.

Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeres[electronic resource] /by Yusuke Kawamoto. - Singapore :Springer Singapore :2019. - xii, 122 p. :ill., digital ;24 cm. - Springer theses,2190-5053. - Springer theses..

1. General Introduction Sequence-specific DNA Binding Pyrrole-Imidazole Polyamides and Their Applications -- 2. Development of a New Method for Synthesis of Tandem Hairpin Pyrrole-Imidazole Polyamide Probes Targeting Human Telomeres -- 3. Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences -- 4. Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole?Imidazole Polyamide Probes.

This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole-imidazole (Py-Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py-Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py-Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py-Im polyamides, particularly the extension of the target sites and improvement of the specificity, which can be applied to the study of pyrrole-imidazole polyamides with higher sequence specificity and the function of telomeres.

ISBN: 9789811369124

Standard No.: 10.1007/978-981-13-6912-4doiSubjects--Topical Terms:

1305703
Pyrroles.

LC Class. No.: QD401 / .K38 2019

Dewey Class. No.: 547.593

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520 $a This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole-imidazole (Py-Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py-Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py-Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py-Im polyamides, particularly the extension of the target sites and improvement of the specificity, which can be applied to the study of pyrrole-imidazole polyamides with higher sequence specificity and the function of telomeres.
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