語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
到查詢結果
[ subject:"Microbiology." ]
切換:
標籤
|
MARC模式
|
ISBD
Inhibition of S. aureus by E. faecal...
~
Platt, Amanda.
FindBook
Google Book
Amazon
博客來
Inhibition of S. aureus by E. faecalis in flow biofilms and transfer of tetracycline resistance gene tetM from E. faecalis to S. typhimurium in mixed species biofilms.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Inhibition of S. aureus by E. faecalis in flow biofilms and transfer of tetracycline resistance gene tetM from E. faecalis to S. typhimurium in mixed species biofilms./
作者:
Platt, Amanda.
面頁冊數:
73 p.
附註:
Source: Masters Abstracts International, Volume: 53-03.
Contained By:
Masters Abstracts International53-03(E).
標題:
Microbiology. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1560185
ISBN:
9781321021349
Inhibition of S. aureus by E. faecalis in flow biofilms and transfer of tetracycline resistance gene tetM from E. faecalis to S. typhimurium in mixed species biofilms.
Platt, Amanda.
Inhibition of S. aureus by E. faecalis in flow biofilms and transfer of tetracycline resistance gene tetM from E. faecalis to S. typhimurium in mixed species biofilms.
- 73 p.
Source: Masters Abstracts International, Volume: 53-03.
Thesis (M.S.)--Temple University, 2014.
Enterococcus faecalis is a serious public health concern because it can cause multi-drug resistant opportunistic infections, spread antibiotic resistance to other bacteria and persist in hospital environments.
ISBN: 9781321021349Subjects--Topical Terms:
536250
Microbiology.
Inhibition of S. aureus by E. faecalis in flow biofilms and transfer of tetracycline resistance gene tetM from E. faecalis to S. typhimurium in mixed species biofilms.
LDR
:04129nmm a2200289 4500
001
2064671
005
20151117142813.5
008
170521s2014 ||||||||||||||||| ||eng d
020
$a
9781321021349
035
$a
(MiAaPQ)AAI1560185
035
$a
AAI1560185
040
$a
MiAaPQ
$c
MiAaPQ
100
1
$a
Platt, Amanda.
$3
3179290
245
1 0
$a
Inhibition of S. aureus by E. faecalis in flow biofilms and transfer of tetracycline resistance gene tetM from E. faecalis to S. typhimurium in mixed species biofilms.
300
$a
73 p.
500
$a
Source: Masters Abstracts International, Volume: 53-03.
500
$a
Adviser: Bettina A. Buttaro.
502
$a
Thesis (M.S.)--Temple University, 2014.
520
$a
Enterococcus faecalis is a serious public health concern because it can cause multi-drug resistant opportunistic infections, spread antibiotic resistance to other bacteria and persist in hospital environments.
520
$a
Static and flow biofilms were used to assess the role of amyloids in transfer of pCF10 from E. faecalis to Staphylococcus aureus. Transfer of tetM from E. faecalis to a clinical isolate of S. aureus, at a rate of 10-8 , was demonstrated by Ella Massie-Schuh using a static biofilm model. However, the recipient strain used in this model did not produce amyloids because the biofilms were grown in nutrient rich TH broth. This model was not successful when an amyloid producing strain of S. aureus was used as the recipient species because the low nutrient PNG medium, needed to induce S. aureus amyloid production, did not support growth of E. faecalis in static biofilms (Schwartz et al., 2012). A flow model presumably resolved nutrient insufficiency because both S. aureus and E. faecalis could be recovered from this model. However, counts of S. aureus were low when grown in mixed species biofilms with E. faecalis as compared to single species biofilms, which suggests that E. faecalis is inhibiting biofilm formation of S. aureus in this model. A mouse skin infection model was also used to examine transfer between E. faecalis and an amyloid producing strain of S. aureus . Unlike the flow biofilm model, E. faecalis did not inhibit the growth of S. aureus in a wound environment. However, no tetracycline resistant S. aureus colonies were recovered.
520
$a
Conical tube biofilms were used to assess transfer of tetM from E. faecalis to E. coli MC4100, EPEC and S. Typhimurium. Mixed species biofilms were grown on glass slides, in conical tubes, for 5 days. In this model, transfer of pCF10 was induced by adding synthetic cCF10. Screening putative recipient colonies showed one transfer event of tetM from E. faecalis to S. Typhimurium. Transfer was confirmed by 16S rRNA sequencing and sequencing of the tetM gene. While this is interesting, it may not be useful as a transfer model because the spontaneous mutation rates approached 10-1 for E. coli, EPEC and S. Typhimurium. In a separate experiment, three mice were given either S. Typhimurium alone, S. Typhimurium with E. faecalis OG1RF(pCF10) or S. Typhimurium with E. faecalis OG1RF(pCF10) and E. faecalis lacking the plasmid. E. faecalis lacking pCF10 was used to provide cCF10 as synthetic cCF10 could not be used in this model. E. faecalis was not recovered from stool samples past day two. Chromosomal DNA was isolated from select S. Typhimurium isolates. None of the colonies tested were shown to be tetM recipients. In addition, the total number of tetracycline resistant S. Typhimurium was close to the total number of S. Typhimurium recovered. Therefore, similar to the conical tube model, this model may not be useful due to the high spontaneous mutation rate. While transfer was not detected using animal models, confirmation of transfer in the conical tube and static models may merit continuation of this project using improved wound and gastrointestinal models. (Abstract shortened by UMI.).
590
$a
School code: 0225.
650
4
$a
Microbiology.
$3
536250
690
$a
0410
710
2
$a
Temple University.
$b
Biomedical Sciences.
$3
3179291
773
0
$t
Masters Abstracts International
$g
53-03(E).
790
$a
0225
791
$a
M.S.
792
$a
2014
793
$a
English
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1560185
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9297381
電子資源
11.線上閱覽_V
電子書
EB
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入