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Animal cell culture: principles and ...

Mani, Shalini.

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Animal cell culture: principles and practice

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Animal cell culture: principles and practice/ by Shalini Mani, Manisha Singh, Anil Kumar.
作者:	Mani, Shalini.
其他作者:	Singh, Manisha.
出版者:	Cham :Springer International Publishing : : 2023.,
面頁冊數:	xvi, 327 p. :ill. (chiefly color), digital ;24 cm.
內容註:	1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques.
Contained By:	Springer Nature eBook
標題:	Cell culture - Laboratory manuals. -
電子資源:	https://doi.org/10.1007/978-3-031-19485-6
ISBN:	9783031194856

Animal cell culture: principles and practice
Mani, Shalini.

Animal cell culture: principles and practice[electronic resource] /by Shalini Mani, Manisha Singh, Anil Kumar. - Cham :Springer International Publishing :2023. - xvi, 327 p. :ill. (chiefly color), digital ;24 cm. - Techniques in life science and biomedicine for the non-expert,2367-1122. - Techniques in life science and biomedicine for the non-expert..

1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques.

This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.

ISBN: 9783031194856

Standard No.: 10.1007/978-3-031-19485-6doiSubjects--Topical Terms:

700715
Cell culture
--Laboratory manuals.

LC Class. No.: QH585.2

Dewey Class. No.: 571.638078

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