語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
到查詢結果
[ subject:"Chemistry, Biochemistry." ]
切換:
標籤
|
MARC模式
|
ISBD
Investigations into the enzymology a...
~
Cheng, Timothy Casey.
FindBook
Google Book
Amazon
博客來
Investigations into the enzymology and biotechnology of the hyperthermophilic carboxypeptidase (PfuCP) from the archaeon Pyrococcus furiosus.
紀錄類型:
書目-語言資料,印刷品 : Monograph/item
正題名/作者:
Investigations into the enzymology and biotechnology of the hyperthermophilic carboxypeptidase (PfuCP) from the archaeon Pyrococcus furiosus./
作者:
Cheng, Timothy Casey.
面頁冊數:
160 p.
附註:
Adviser: Sunney I. Chan.
Contained By:
Dissertation Abstracts International64-01B.
標題:
Chemistry, Biochemistry. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3077718
ISBN:
0493982272
Investigations into the enzymology and biotechnology of the hyperthermophilic carboxypeptidase (PfuCP) from the archaeon Pyrococcus furiosus.
Cheng, Timothy Casey.
Investigations into the enzymology and biotechnology of the hyperthermophilic carboxypeptidase (PfuCP) from the archaeon Pyrococcus furiosus.
- 160 p.
Adviser: Sunney I. Chan.
Thesis (Ph.D.)--California Institute of Technology, 2003.
The broad amino acid specificity and rapid digestion by PfuCP in peptide sequencing trials show promise, and high-temperature protein sequencing has now been demonstrated for the first time.
ISBN: 0493982272Subjects--Topical Terms:
1017722
Chemistry, Biochemistry.
Investigations into the enzymology and biotechnology of the hyperthermophilic carboxypeptidase (PfuCP) from the archaeon Pyrococcus furiosus.
LDR
:03134nam 2200313 a 45
001
926946
005
20110422
008
110422s2003 eng d
020
$a
0493982272
035
$a
(UnM)AAI3077718
035
$a
AAI3077718
040
$a
UnM
$c
UnM
100
1
$a
Cheng, Timothy Casey.
$3
1250528
245
1 0
$a
Investigations into the enzymology and biotechnology of the hyperthermophilic carboxypeptidase (PfuCP) from the archaeon Pyrococcus furiosus.
300
$a
160 p.
500
$a
Adviser: Sunney I. Chan.
500
$a
Source: Dissertation Abstracts International, Volume: 64-01, Section: B, page: 0185.
502
$a
Thesis (Ph.D.)--California Institute of Technology, 2003.
520
$a
The broad amino acid specificity and rapid digestion by PfuCP in peptide sequencing trials show promise, and high-temperature protein sequencing has now been demonstrated for the first time.
520
$a
A novel metallocarboxypeptidase (PfuCP) from the hyperthermophilic archaeon <italic> Pyrococcus furiosus</italic> was purified and characterized to investigate its dependence on metal ion cofactors and to evaluate its suitability as a biotechnological tool for protein sequencing at elevated temperatures.
520
$a
The crystal structure reveals a dimer of primarily α-helical subunits that bears no resemblance to the α/β-hydrolase morphology of typical carboxypeptidases and which defines a new family of HEXXH metalloproteases (M32) based on primary sequence alignments. A deep active site groove appears to function not only in size-selection of substrates but also in modulating the activity and substrate affinity through complicated allosteric effects involving ambient ligands which may play a role in regulatory metabolism.
520
$a
Two forms of the enzyme were observed; one which retains stabilizing metal(s) that confer structural thermostability and a remarkable retention of activity to the dimer, and another demetallated form which has lost stability with regards to both dimeric integrity and activity. Difficulties in expressing a properly folded recombinant necessitated refolding of the expressed clone from inclusion bodies and further suggest that <italic>in vivo</italic> the stabilizing metal(s) may participate in folding a metastable enzyme.
520
$a
The apparent paradox of activation by only Co<super>2+</super> and not Zn<super>2+</super> is resolved into two issues, uncompetitive inhibition by the latter as seen in steady-state kinetic experiments, and intrinsic, electronic aspects of a catalytic Co<super>2+</super>. Several explanations are proposed for the intrinsic rate enhancement of Co<super>2+</super> over Zn<super>2+</super> including the ability of Co<super>2+</super> to modulate the potential energy surface for both reactants and transition states by virtue of its greater mobility within the protein framework.
590
$a
School code: 0037.
650
4
$a
Chemistry, Biochemistry.
$3
1017722
690
$a
0487
710
2 0
$a
California Institute of Technology.
$3
726902
773
0
$t
Dissertation Abstracts International
$g
64-01B.
790
$a
0037
790
1 0
$a
Chan, Sunney I.,
$e
advisor
791
$a
Ph.D.
792
$a
2003
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3077718
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9098903
電子資源
11.線上閱覽_V
電子書
EB W9098903
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入