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[ subject:"Chemistry, Analytical." ]
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Alternating current conductance of s...
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The University of Utah.
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Alternating current conductance of synthetic and biological nanopores.
紀錄類型:
書目-語言資料,印刷品 : Monograph/item
正題名/作者:
Alternating current conductance of synthetic and biological nanopores./
作者:
Ervin, Eric Nathan.
面頁冊數:
193 p.
附註:
Adviser: Henry S. White.
Contained By:
Dissertation Abstracts International68-07B.
標題:
Chemistry, Analytical. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3271435
ISBN:
9780549105817
Alternating current conductance of synthetic and biological nanopores.
Ervin, Eric Nathan.
Alternating current conductance of synthetic and biological nanopores.
- 193 p.
Adviser: Henry S. White.
Thesis (Ph.D.)--The University of Utah, 2007.
The application of an alternating current (AC) signal as a means to investigate the conductance of synthetic and biological nanometer-dimension pores is developed. Although the objective of this dissertation is to develop AC methodology, using lock-in amplifier techniques for ion-channel recordings, to measure the impedance of single nanopores, the specific goal is to measure binding events between the transmembrane protein alpha-hemolysin (alphaHL) and an analyte molecule, while using a synthetic platform support.
ISBN: 9780549105817Subjects--Topical Terms:
586156
Chemistry, Analytical.
Alternating current conductance of synthetic and biological nanopores.
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Thesis (Ph.D.)--The University of Utah, 2007.
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The application of an alternating current (AC) signal as a means to investigate the conductance of synthetic and biological nanometer-dimension pores is developed. Although the objective of this dissertation is to develop AC methodology, using lock-in amplifier techniques for ion-channel recordings, to measure the impedance of single nanopores, the specific goal is to measure binding events between the transmembrane protein alpha-hemolysin (alphaHL) and an analyte molecule, while using a synthetic platform support.
520
$a
Chapters 3 and 4 present AC impedance imaging of synthetic nanoporous membranes using scanning electrochemical microscopy (SECM). The impedance image "quality" of single conical-shaped membrane nanopores was determined to be inversely proportional to the resistance of the membrane. Pores in high resistance membranes (i.e., membranes containing a single pore) could not be imaged without the use of a bypass channel, which effectively creates an electrical short around the membrane.
520
$a
Chapter 5 describes the AC conductance of the transmembrane proteins alphaHL and outer membrane protein F (OmpF), using conventional planar lipid bilayer techniques. A method of analyzing the conductance vs. KCI concentration plots is introduced that allows the determination of the concentration of counter-ions associated with ionizable groups inside the lumen of the protein without knowledge of either the protein geometry or the ion mobilities.
520
$a
In Chapter 6, AC lock-in techniques and the glass nanopore membrane are combined to perform single molecule detection using a protein ion channel. This is the first reported demonstration where the binding interaction between an analyte molecule and a protein pore are measured at zero DC bias, resulting in minimal effects of capture and dwell time on electrophoresis and electroosmosis. Furthermore, the superposition of a DC bias onto the AC signal is used to control the binding of a highly charged molecule to the protein channel. This methodology is used in Chapter 7 to probe the kinetics of binding between alphaHL and a sulfonated-beta-cyclodextrin molecule.
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$a
The results in this dissertation demonstrate the ability to use an AC signal, to monitor the conductance of a nanopore. With the appropriate choice of reference frequency and low-pass filter time constant, a low-noise AC measurement may be made, that has significant fundamental advantages over a conventional DC measurement.
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