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[ subject:"Biophysics, General." ]
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Nanopore analysis of nucleic acids.
~
Butler, Thomas.
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Nanopore analysis of nucleic acids.
紀錄類型:
書目-語言資料,印刷品 : Monograph/item
正題名/作者:
Nanopore analysis of nucleic acids./
作者:
Butler, Thomas.
面頁冊數:
106 p.
附註:
Adviser: Jens H. Gundlach.
Contained By:
Dissertation Abstracts International68-05B.
標題:
Biophysics, General. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3265304
ISBN:
9780549038917
Nanopore analysis of nucleic acids.
Butler, Thomas.
Nanopore analysis of nucleic acids.
- 106 p.
Adviser: Jens H. Gundlach.
Thesis (Ph.D.)--University of Washington, 2007.
Nanopore analysis of nucleic acids is a novel technique to study the physical properties of nucleic acids at the single-molecule level. It also has the potential to be a central component of a fundamentally new DNA sequencing methodology. In this technique, individual single-stranded DNA or RNA molecules are detected and characterized by an ionic current blockade produced when they are electrophoretically driven through a nanometer-scale pore. The primary focus of this thesis is a detailed characterization and interpretation of the sub-level structure of the ionic current blockades produced during the interaction between single-stranded DNA and RNA molecules and the Staphylococcus aureus alpha-Hemolysin protein nanopore. These investigations reveal important mechanistic details of the interaction between nucleic acids and alpha-Hemolysin. The results presented in this thesis contribute significantly to the growing utility of nanopore analysis as a model system to study biologically relevant physical and chemical processes, and they may help with the development of novel, nanopore-based technological applications. A secondary focus of this thesis is the initial steps toward genetically engineering Mycobacterium smegmatis porin A as an alternative protein nanopore for nucleic acid analysis. Successful creation of an additional protein nanopore represents a substantial scientific and technological breakthrough.
ISBN: 9780549038917Subjects--Topical Terms:
1019105
Biophysics, General.
Nanopore analysis of nucleic acids.
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Nanopore analysis of nucleic acids is a novel technique to study the physical properties of nucleic acids at the single-molecule level. It also has the potential to be a central component of a fundamentally new DNA sequencing methodology. In this technique, individual single-stranded DNA or RNA molecules are detected and characterized by an ionic current blockade produced when they are electrophoretically driven through a nanometer-scale pore. The primary focus of this thesis is a detailed characterization and interpretation of the sub-level structure of the ionic current blockades produced during the interaction between single-stranded DNA and RNA molecules and the Staphylococcus aureus alpha-Hemolysin protein nanopore. These investigations reveal important mechanistic details of the interaction between nucleic acids and alpha-Hemolysin. The results presented in this thesis contribute significantly to the growing utility of nanopore analysis as a model system to study biologically relevant physical and chemical processes, and they may help with the development of novel, nanopore-based technological applications. A secondary focus of this thesis is the initial steps toward genetically engineering Mycobacterium smegmatis porin A as an alternative protein nanopore for nucleic acid analysis. Successful creation of an additional protein nanopore represents a substantial scientific and technological breakthrough.
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http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3265304
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