語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
到查詢結果
[ subject:"Biology, Anatomy." ]
切換:
標籤
|
MARC模式
|
ISBD
Somitogenesis in zebrafish.
~
Henry, Clarissa Ann.
FindBook
Google Book
Amazon
博客來
Somitogenesis in zebrafish.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Somitogenesis in zebrafish./
作者:
Henry, Clarissa Ann.
面頁冊數:
94 p.
附註:
Source: Dissertation Abstracts International, Volume: 61-11, Section: B, page: 5666.
Contained By:
Dissertation Abstracts International61-11B.
標題:
Biology, Cell. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9995376
ISBN:
0493039139
Somitogenesis in zebrafish.
Henry, Clarissa Ann.
Somitogenesis in zebrafish.
- 94 p.
Source: Dissertation Abstracts International, Volume: 61-11, Section: B, page: 5666.
Thesis (Ph.D.)--University of Washington, 2000.
Vertebrate segmentation involves the partitioning of paraxial mesoderm into numerous metameric units, known as somites. We have analyzed the cellular mechanics underlying early somitogenesis in wild-type zebrafish and <italic> trilobite, knypek</italic>, and <italic>knypek;trilobite</italic> mutants. We show that the formation of somite boundaries in all of these embryos involves segregation, local alignment, and cell shape changes of presumptive epitheloid border cells along nascent intersomitic boundaries. <italic>knypek;trilobite </italic> mutant embryos are extreme morphological variants whose somites lack internal mesenchymal cells, and form without convergence of the presomitic mesoderm. Although <italic>knypek;trilobite</italic> somites are composed of only two cells in their anterior-posterior dimension, they still exhibit anterior posterior intrasegmental polarity. Furthermore, morphogenesis of somite boundaries in these embryos proceeds in a manner similar to wild-type embryos. These results indicate that intersomitic boundary formation in zebrafish involves short-range movements of presumptive border cells that do not require mechanical forces generated by internal cells or compaction of the presomitic mesoderm. In order to understand how cytoskeleton-extracellular matrix interactions regulate cell movements during development, we have cloned zebrafish focal adhesion kinase (Fak), and analyzed its subcellular localization. In the axial mesoderm, Fak protein is localized to the cortex of notochord cells and is frequently present in large plaques at the cortex. During somitogenesis, Fak protein becomes concentrated at the basal region of epithelial somite cells. The wild-type segmental pattern of <italic>fak</italic> mRNA expression in the paraxial mesoderm is not dependent upon Notch signaling through <italic> Suppressor of Hairless</italic> (<italic>SuH</italic>), <italic>after eight </italic>/<italic>deltaD</italic>, or upon the activity of <italic>deadly seven</italic>. Taken together, these results suggest a dual role for FAK in mesoderm morphogenesis: (1) FAK may facilitate notochord cell intercalation, and (2) FAK may play a role in the formation and stabilization of somite boundaries.
ISBN: 0493039139Subjects--Topical Terms:
1017686
Biology, Cell.
Somitogenesis in zebrafish.
LDR
:03150nmm 2200289 4500
001
1810456
005
20040311140853.5
008
130610s2000 eng d
020
$a
0493039139
035
$a
(UnM)AAI9995376
035
$a
AAI9995376
040
$a
UnM
$c
UnM
100
1
$a
Henry, Clarissa Ann.
$3
1900067
245
1 0
$a
Somitogenesis in zebrafish.
300
$a
94 p.
500
$a
Source: Dissertation Abstracts International, Volume: 61-11, Section: B, page: 5666.
500
$a
Co-Chairs: Mark S. Cooper; Merrill B. Hille.
502
$a
Thesis (Ph.D.)--University of Washington, 2000.
520
$a
Vertebrate segmentation involves the partitioning of paraxial mesoderm into numerous metameric units, known as somites. We have analyzed the cellular mechanics underlying early somitogenesis in wild-type zebrafish and <italic> trilobite, knypek</italic>, and <italic>knypek;trilobite</italic> mutants. We show that the formation of somite boundaries in all of these embryos involves segregation, local alignment, and cell shape changes of presumptive epitheloid border cells along nascent intersomitic boundaries. <italic>knypek;trilobite </italic> mutant embryos are extreme morphological variants whose somites lack internal mesenchymal cells, and form without convergence of the presomitic mesoderm. Although <italic>knypek;trilobite</italic> somites are composed of only two cells in their anterior-posterior dimension, they still exhibit anterior posterior intrasegmental polarity. Furthermore, morphogenesis of somite boundaries in these embryos proceeds in a manner similar to wild-type embryos. These results indicate that intersomitic boundary formation in zebrafish involves short-range movements of presumptive border cells that do not require mechanical forces generated by internal cells or compaction of the presomitic mesoderm. In order to understand how cytoskeleton-extracellular matrix interactions regulate cell movements during development, we have cloned zebrafish focal adhesion kinase (Fak), and analyzed its subcellular localization. In the axial mesoderm, Fak protein is localized to the cortex of notochord cells and is frequently present in large plaques at the cortex. During somitogenesis, Fak protein becomes concentrated at the basal region of epithelial somite cells. The wild-type segmental pattern of <italic>fak</italic> mRNA expression in the paraxial mesoderm is not dependent upon Notch signaling through <italic> Suppressor of Hairless</italic> (<italic>SuH</italic>), <italic>after eight </italic>/<italic>deltaD</italic>, or upon the activity of <italic>deadly seven</italic>. Taken together, these results suggest a dual role for FAK in mesoderm morphogenesis: (1) FAK may facilitate notochord cell intercalation, and (2) FAK may play a role in the formation and stabilization of somite boundaries.
590
$a
School code: 0250.
650
4
$a
Biology, Cell.
$3
1017686
650
4
$a
Biology, Anatomy.
$3
1021727
690
$a
0379
690
$a
0287
710
2 0
$a
University of Washington.
$3
545923
773
0
$t
Dissertation Abstracts International
$g
61-11B.
790
1 0
$a
Cooper, Mark S.,
$e
advisor
790
1 0
$a
Hille, Merrill B.,
$e
advisor
790
$a
0250
791
$a
Ph.D.
792
$a
2000
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9995376
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9171194
電子資源
11.線上閱覽_V
電子書
EB
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入