語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
到查詢結果
[ subject:"Animal Physiology." ]
切換:
標籤
|
MARC模式
|
ISBD
The mechanism of osteoinduction by n...
~
Ni, Ming.
FindBook
Google Book
Amazon
博客來
The mechanism of osteoinduction by nacre: Effects of soluble proteins and insoluble matrix.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
The mechanism of osteoinduction by nacre: Effects of soluble proteins and insoluble matrix./
作者:
Ni, Ming.
面頁冊數:
182 p.
附註:
Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 5273.
Contained By:
Dissertation Abstracts International65-10B.
標題:
Chemical engineering. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3151648
ISBN:
9780496121267
The mechanism of osteoinduction by nacre: Effects of soluble proteins and insoluble matrix.
Ni, Ming.
The mechanism of osteoinduction by nacre: Effects of soluble proteins and insoluble matrix.
- 182 p.
Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 5273.
Thesis (Ph.D.)--University of Washington, 2004.
Nacre, the inner layer of mollucan shell, has attracted increasing attention over recent years as a potential material for bone and/or tooth implants. As shown in previous studies, nacre has osteoinductive properties causing it induce bone formation. But how and why nacre can induce bone formation remains unclear. The objective of this work is to determine the mechanism of nacre's osteoinudctive properties. Two alternative mechanisms have already been proposed to explain the osteogenic properties of nacre: (1) either nacre binds to bone via a hydroxyapatite layer or (2) the presence of soluble nacre proteins stimulates osteoblast differentiation.
ISBN: 9780496121267Subjects--Topical Terms:
560457
Chemical engineering.
The mechanism of osteoinduction by nacre: Effects of soluble proteins and insoluble matrix.
LDR
:03176nmm a2200337 4500
001
2115204
005
20170217105129.5
008
180830s2004 ||||||||||||||||| ||eng d
020
$a
9780496121267
035
$a
(MiAaPQ)AAI3151648
035
$a
AAI3151648
040
$a
MiAaPQ
$c
MiAaPQ
100
1
$a
Ni, Ming.
$3
1921272
245
1 4
$a
The mechanism of osteoinduction by nacre: Effects of soluble proteins and insoluble matrix.
300
$a
182 p.
500
$a
Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 5273.
500
$a
Chairperson: Buddy D. Ratner.
502
$a
Thesis (Ph.D.)--University of Washington, 2004.
520
$a
Nacre, the inner layer of mollucan shell, has attracted increasing attention over recent years as a potential material for bone and/or tooth implants. As shown in previous studies, nacre has osteoinductive properties causing it induce bone formation. But how and why nacre can induce bone formation remains unclear. The objective of this work is to determine the mechanism of nacre's osteoinudctive properties. Two alternative mechanisms have already been proposed to explain the osteogenic properties of nacre: (1) either nacre binds to bone via a hydroxyapatite layer or (2) the presence of soluble nacre proteins stimulates osteoblast differentiation.
520
$a
In order to test those postulated mechanisms, nacre was first separated from abalone shell and soaked in a phosphate buffer solution to determine if nacre has the ability to surface-transform to hydroxyapatite (HAP). HAP formation is desirable for a bone/tooth implant because it can form strong bonds with natural bone. The mechanism of this surface transformation can be explained as a dissolution-precipitation mechanism.
520
$a
Second, soluble nacre proteins were isolated and separated from nacre by acetic acid treatment. Those proteins were analyzed by amino acid analysis and matrix-assisted laser desorption/ionization mass spectrometry. The effects of the resulting proteins on osteoblast differentiation were also studied. Soluble nacre protein increased alkaline phosphatase (ALP) activity and osteopontin (OPN) expression in cultures of osteoblasts. Since both ALP and OPN are markers of osteoblast differentiation, our results support the hypothesis that nacre protein promotes the differentiation of osteoblast in vitro.
520
$a
A material model that mimics nacre was also developed via a stepwise formation approach. The thickness of organic layer was about 10 nm and that of inorganic layer was on the scale of microns. Secondary ion mass spectrometry data were analyzed to reveal each layer of this multilayered material. The osteoblasts' response to this material model was also evaluated by cell adhesion and proliferation. It showed that the number of cells on this material model was comparable to nacre after 14-day culture.
590
$a
School code: 0250.
650
4
$a
Chemical engineering.
$3
560457
650
4
$a
Biomedical engineering.
$3
535387
650
4
$a
Biochemistry.
$3
518028
650
4
$a
Animal Physiology.
$3
894174
690
$a
0542
690
$a
0541
690
$a
0487
690
$a
0433
710
2
$a
University of Washington.
$3
545923
773
0
$t
Dissertation Abstracts International
$g
65-10B.
790
$a
0250
791
$a
Ph.D.
792
$a
2004
793
$a
English
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3151648
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9325825
電子資源
01.外借(書)_YB
電子書
EB
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入