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Somatic embryogenesis of Phalaenopsis.

Kim, Se-Young.

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Somatic embryogenesis of Phalaenopsis.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Somatic embryogenesis of Phalaenopsis./
Author:	Kim, Se-Young.
Description:	156 p.
Notes:	Chairman: Yoneo Sagawa.
Contained By:	Dissertation Abstracts International55-06B.
Subject:	Agriculture, Plant Culture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9429625

Somatic embryogenesis of Phalaenopsis.
Kim, Se-Young.

Somatic embryogenesis of Phalaenopsis. - 156 p.

Chairman: Yoneo Sagawa.

Thesis (Ph.D.)--University of Hawai'i at Manoa, 1994.

Regeneration of plantlets through indirect somatic embryogenesis is shown to be a viable pathway for clonal propagation of recalcitrant Phalaenopsis orchids.Subjects--Topical Terms:

1018669
Agriculture, Plant Culture.

Somatic embryogenesis of Phalaenopsis.
LDR:03221nam 2200337 a 45 001 973107
005 20110928
008 110928s1994 eng d
035 $a (UMI)AAI9429625
035 $a AAI9429625
040 $a UMI $c UMI
100 1 $a Kim, Se-Young. $3 1297066
245 1 0 $a Somatic embryogenesis of Phalaenopsis.
300 $a 156 p.
500 $a Chairman: Yoneo Sagawa.
500 $a Source: Dissertation Abstracts International, Volume: 55-06, Section: B, page: 2048.
502 $a Thesis (Ph.D.)--University of Hawai'i at Manoa, 1994.
520 $a Regeneration of plantlets through indirect somatic embryogenesis is shown to be a viable pathway for clonal propagation of recalcitrant Phalaenopsis orchids.
520 $a The growth and differentiation of a unique Phalaenopsis callus was characterized. The callus was determined to be friable and embryogenic producing plantlets which matured and flowered.
520 $a In contrast to other orchid calli reported previously, the callus was unique in its extreme friability and undifferentiation with no trace of organization. The callus was maintained on a modified Vacin and Went medium containing sucrose 20.0 g$\cdot $l iter$\sp{-1}$ supplemented with coconut water (CW) 150 ml$\cdot $l iter$\sp{-1}$ without any morphological change for more than five years. Analyses of callus growth (fresh weight) showed that the callus had an extremely low growth rate, which was not affected by sucrose levels between 2.5 to 30.0 g$\cdot $l iter$\sp{-1}$. Although CW stimulated callus growth, IAA (indole-3-acetic acid) or BA (benzyl adenine) was not effective.
520 $a Somatic embryogenesis was induced by lowering sucrose level to 2.5 g$\cdot $l iter$\sp{-1}$ or deletion of sucrose and supplementing with CW. Callus turned green and subsequently formed numerous embryoids at periphery of green callus. The development of embryoids into plantlets morphologically resembled germination of zygotic embryos of Phalaenopsis.
520 $a Sucrose (20.0 g$\cdot $l iter$\sp{-1}$) inhibited greening and somatic embryogenesis. Sugar inhibition of greening and somatic embryogenesis was not overcome by transfer of callus from auxin-enriched medium to auxin-free medium or increasing nitrogen level, but only by reduction of carbohydrates available in medium. Pretreatment with IAA or NAA ($\alpha$-naphthaleneacetic acid), and supplement of CW, casein hydrolysate (CH), glutamic acid or glutamine with 2.5 g$\cdot $l iter$\sp{-1}$ of sucrose were marginally effective in promoting embryogenesis.
520 $a Readily utilizable monosaccharides, glucose, fructose, mannose, ribose, xylose and disaccharide, sucrose at 20.0 g$\cdot $l iter$\sp{-1}$ were inhibitory, while other disaccharides, multisaccharides and sugar-alcohols supported greening and somatic embryogenesis. Amylose (20.0 g$\cdot $l iter$\sp{-1}$) was the most effective in induction of embryogenesis among carbohydrate sources.
590 $a School code: 0085.
650 4 $a Agriculture, Plant Culture. $3 1018669
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Plant Physiology. $3 1017865
690 $a 0379
690 $a 0479
690 $a 0817
710 2 0 $a University of Hawai'i at Manoa. $3 1017511
773 0 $t Dissertation Abstracts International $g 55-06B.
790 $a 0085
790 1 0 $a Sagawa, Yoneo, $e advisor
791 $a Ph.D.
792 $a 1994
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9429625