東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Real-time RT-PCR quantification of p...

Aad, Pauline Youssef.

Linked to FindBook

Google Book

Amazon

博客來

Real-time RT-PCR quantification of pregnancy-associated plasma protein-A gene expression in granulosa and theca cells: Effects of hormones in vitro.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Real-time RT-PCR quantification of pregnancy-associated plasma protein-A gene expression in granulosa and theca cells: Effects of hormones in vitro./
Author:	Aad, Pauline Youssef.
Description:	98 p.
Notes:	Adviser: Leon J. Spicer.
Contained By:	Masters Abstracts International43-05.
Subject:	Biology, Animal Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1431126
ISBN:	9780542038785

Real-time RT-PCR quantification of pregnancy-associated plasma protein-A gene expression in granulosa and theca cells: Effects of hormones in vitro.
Aad, Pauline Youssef.

Real-time RT-PCR quantification of pregnancy-associated plasma protein-A gene expression in granulosa and theca cells: Effects of hormones in vitro. - 98 p.

Adviser: Leon J. Spicer.

Thesis (M.S.)--Oklahoma State University, 2005.

Ovarian follicular growth and dominance is controlled by a series of hormonal events including a decrease in intrafollicular IGF-binding proteins-2, -4 and -5 levels. Proteolytic enzymes such as pregnancy-associated plasma protein-A (PAPP-A) degrade IGFBPs and increase availability of IGF-I and -II during follicular development. The objective of this study was to determine the effect of IGF-I, IGF-II, insulin (INS), LH, FSH, estradiol (E), leptin or cortisol on ovarian PAPP-A mRNA levels. Granulosa (GC) from small (SM) (<5 mm) and large (LG) (>7.9 mm) follicles as well as theca cells (TC) from LG follicles were collected from bovine ovaries and cultured for 48 h in medium containing 10% FCS and then treated with various hormones in serum-free medium for an additional 12 or 24 h. LG-GC and LG-TC were cultured for either 12 or 24 h with 0 or 100 ng/mL of IGF-I or -II (exp 1 and 2). In all other experiments, SM-GC LG-GC and LG-TC were treated with various combinations of IGF-I, IGF-II, FSH, LH, E, INS, leptin and (or) cortisol for 24 h (exp 3 through 12). PAPP-A mRNA expression levels quantified using quantitative real-time RT-PCR. (Abstract shortened by UMI.)

ISBN: 9780542038785Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Real-time RT-PCR quantification of pregnancy-associated plasma protein-A gene expression in granulosa and theca cells: Effects of hormones in vitro.
LDR:02105nam 2200277 a 45 001 973018
005 20110928
008 110928s2005 eng d
020 $a 9780542038785
035 $a (UnM)AAI1431126
035 $a AAI1431126
040 $a UnM $c UnM
100 1 $a Aad, Pauline Youssef. $3 1296977
245 1 0 $a Real-time RT-PCR quantification of pregnancy-associated plasma protein-A gene expression in granulosa and theca cells: Effects of hormones in vitro.
300 $a 98 p.
500 $a Adviser: Leon J. Spicer.
500 $a Source: Masters Abstracts International, Volume: 43-05, page: 1651.
502 $a Thesis (M.S.)--Oklahoma State University, 2005.
520 $a Ovarian follicular growth and dominance is controlled by a series of hormonal events including a decrease in intrafollicular IGF-binding proteins-2, -4 and -5 levels. Proteolytic enzymes such as pregnancy-associated plasma protein-A (PAPP-A) degrade IGFBPs and increase availability of IGF-I and -II during follicular development. The objective of this study was to determine the effect of IGF-I, IGF-II, insulin (INS), LH, FSH, estradiol (E), leptin or cortisol on ovarian PAPP-A mRNA levels. Granulosa (GC) from small (SM) (<5 mm) and large (LG) (>7.9 mm) follicles as well as theca cells (TC) from LG follicles were collected from bovine ovaries and cultured for 48 h in medium containing 10% FCS and then treated with various hormones in serum-free medium for an additional 12 or 24 h. LG-GC and LG-TC were cultured for either 12 or 24 h with 0 or 100 ng/mL of IGF-I or -II (exp 1 and 2). In all other experiments, SM-GC LG-GC and LG-TC were treated with various combinations of IGF-I, IGF-II, FSH, LH, E, INS, leptin and (or) cortisol for 24 h (exp 3 through 12). PAPP-A mRNA expression levels quantified using quantitative real-time RT-PCR. (Abstract shortened by UMI.)
590 $a School code: 0664.
650 4 $a Biology, Animal Physiology. $3 1017835
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0433
710 2 0 $a Oklahoma State University. $3 626630
773 0 $t Masters Abstracts International $g 43-05.
790 $a 0664
790 1 0 $a Spicer, Leon J., $e advisor
791 $a M.S.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1431126