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Characterization of CR1L gene expres...

Irshaid, Fawzi Irshaid.

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Characterization of CR1L gene expression on human tissues: Identification of CR1L-2, a two SCR transcript from human fetal liver and bone marrow.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Characterization of CR1L gene expression on human tissues: Identification of CR1L-2, a two SCR transcript from human fetal liver and bone marrow./
作者:	Irshaid, Fawzi Irshaid.
面頁冊數:	138 p.
附註:	Adviser: Daniel J. Birmingham.
Contained By:	Dissertation Abstracts International66-01B.
標題:	Biology, Genetics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3161132
ISBN:	9780496944293

Characterization of CR1L gene expression on human tissues: Identification of CR1L-2, a two SCR transcript from human fetal liver and bone marrow.
Irshaid, Fawzi Irshaid.

Characterization of CR1L gene expression on human tissues: Identification of CR1L-2, a two SCR transcript from human fetal liver and bone marrow. - 138 p.

Adviser: Daniel J. Birmingham.

Thesis (Ph.D.)--The Ohio State University, 2005.

CR1L is a genetic element identified from human genomic clones with high homology to complement receptor type one (CR1). The purpose of this study was to further characterize the expression of human CR1L.

ISBN: 9780496944293Subjects--Topical Terms:

1017730
Biology, Genetics.

Characterization of CR1L gene expression on human tissues: Identification of CR1L-2, a two SCR transcript from human fetal liver and bone marrow.
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100 1 $a Irshaid, Fawzi Irshaid. $3 1296773
245 1 0 $a Characterization of CR1L gene expression on human tissues: Identification of CR1L-2, a two SCR transcript from human fetal liver and bone marrow.
300 $a 138 p.
500 $a Adviser: Daniel J. Birmingham.
500 $a Source: Dissertation Abstracts International, Volume: 66-01, Section: B, page: 0106.
502 $a Thesis (Ph.D.)--The Ohio State University, 2005.
520 $a CR1L is a genetic element identified from human genomic clones with high homology to complement receptor type one (CR1). The purpose of this study was to further characterize the expression of human CR1L.
520 $a In this study, evidence was found for two novel human transcripts in premade northern blots containing poly (A) mRNA from human tissues that were hybridized at high stringency condition with probes derived from the predicted human CR1L gene. The first was implicated through northern blot by discovery of a 1.2 kb transcript in human pancreas. The second novel transcript of 0.7 kb, was also revealed initially through northern blot analysis, with predominant expression in human fetal liver and bone marrow. This transcript, which we've termed CR1L-2, contains a short single open reading frame (ORF) of 525 by that encodes 174 amino acids. The deduced amino acid sequence of the ORF contains a sequence for a signal peptide, SCRs 1 and 2 of CR1L, and a hydrophobic C-terminal predictive of a GPI-link protein followed by a stop codon.
520 $a The deduced amino acid sequence also contains two potential N-glycosylation sites, and many serine and threonine residues which might be potential O-glycosylation sites. The predicted peptide molecular weight of mature CR1L-2 without glycosylation is &sim;16 kDa and has a calculated isoelectric point of 8.5. Comparison of human CR1L-2 sequence with human genome database revealed that the sequence immediately down stream of SCR2 is sequence derived from the adjacent intron, suggesting a read-through at that splice site. Further analysis of the 3 ' end of CR1L-2 demonstrates the presence of a consensus poly (A) signal immediately after the stop codon, and this is followed by an extra 12 nucleotides and a poly (A) tail.
520 $a To date, no immunologic reagents with specificity towards CR1L-2 are available, thus preventing the demonstration of native expression of this abundant transcript. Expression of recombinant CR1L-2 in HEK-293 and COS-7 cells, with C-terminal epitopes (v5 and poly-his), demonstrates a protein of 28 kDa. Taking into account the C-terminal epitope suggests a molecular weight of 23 kDa for the mature CR1L-2 protein.
520 $a In conclusion, we have demonstrated the presence of a full-length cDNA transcript that is restricted to fetal liver and bone marrow. The highly restricted expression suggests that CR1L-2 might play a very specialized role in early lymphogenesis or hematopoiesis. The presence of a hydrophobic C terminal end suggests that CR1L-2 might be expressed as a GPI-linked protein. The potential role of CR1L-2 in binding C4d suggests that this novel protein might play a role in the modulating effect that C4 has on the immune response, especially with regards to the developing B cell repertoire and to C4-based susceptibility to autoimmune diseases such as systemic lupus erythematosus. (Abstract shortened by UMI.)
590 $a School code: 0168.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
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710 2 0 $a The Ohio State University. $3 718944
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790 $a 0168
790 1 0 $a Birmingham, Daniel J., $e advisor
791 $a Ph.D.
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