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Evaluation of molecular typing and i...

Chou, Chung-Hsi.

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Evaluation of molecular typing and immunological responses of Listeria monocytogenes.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Evaluation of molecular typing and immunological responses of Listeria monocytogenes./
Author:	Chou, Chung-Hsi.
Description:	149 p.
Notes:	Adviser: Chinling Wang.
Contained By:	Dissertation Abstracts International68-07B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3270471
ISBN:	9780549097624

Evaluation of molecular typing and immunological responses of Listeria monocytogenes.
Chou, Chung-Hsi.

Evaluation of molecular typing and immunological responses of Listeria monocytogenes. - 149 p.

Adviser: Chinling Wang.

Thesis (Ph.D.)--Mississippi State University, 2007.

Listeria monocytogenes is a causative agent of listeriosis and a deadly food-borne pathogen recognized as a significant public health problem in the United States. Prevention of food contamination as well as the development of efficient prevention and therapeutic strategies have been priorities for the food industry, regulatory agencies and the health research groups. Three specific aims were accomplished in efforts for the aforementioned in this study. The first aim was to evaluate a rapid molecular finger-printing technique, repetitive element sequence-based PCR (REP-PCR) for subtyping L. monocytogenes by comparing it to the gold standard method, pulse-field gel electrophoresis (PFGE). The results showed that the REP-PCR not only had good reproducibility but also provided a similar level of discriminatory ability as the PFGE. Since the REP-PCR is less expensive and more rapid than the PFGE, it could be considered as an alternative molecular typing method for L. monocytogenes.

ISBN: 9780549097624Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Evaluation of molecular typing and immunological responses of Listeria monocytogenes.
LDR:03255nam 2200301 a 45 001 969710
005 20110920
008 110921s2007 eng d
020 $a 9780549097624
035 $a (UMI)AAI3270471
035 $a AAI3270471
040 $a UMI $c UMI
100 1 $a Chou, Chung-Hsi. $3 1293767
245 1 0 $a Evaluation of molecular typing and immunological responses of Listeria monocytogenes.
300 $a 149 p.
500 $a Adviser: Chinling Wang.
500 $a Source: Dissertation Abstracts International, Volume: 68-07, Section: B, page: .
502 $a Thesis (Ph.D.)--Mississippi State University, 2007.
520 $a Listeria monocytogenes is a causative agent of listeriosis and a deadly food-borne pathogen recognized as a significant public health problem in the United States. Prevention of food contamination as well as the development of efficient prevention and therapeutic strategies have been priorities for the food industry, regulatory agencies and the health research groups. Three specific aims were accomplished in efforts for the aforementioned in this study. The first aim was to evaluate a rapid molecular finger-printing technique, repetitive element sequence-based PCR (REP-PCR) for subtyping L. monocytogenes by comparing it to the gold standard method, pulse-field gel electrophoresis (PFGE). The results showed that the REP-PCR not only had good reproducibility but also provided a similar level of discriminatory ability as the PFGE. Since the REP-PCR is less expensive and more rapid than the PFGE, it could be considered as an alternative molecular typing method for L. monocytogenes.
520 $a The second aim was to investigate the subtypes of L. monocytogenes found on raw catfish fillets in three unrelated processing plants. Forty electrophoretic subtypes were distinguished from a total of 97 isolates. From this, 76 isolates were determined as plant-specific, 20 were non-plant-specific, 45 were single-seasonal-distributed and 52 were multi-seasonal-distributed. The results suggest that REP-PCR can be used in tracing the source(s) of contamination and monitoring the effectiveness of sanitation procedures in processing plants.
520 $a The third aim was to investigate the immunological responses of human brain microvascular endothelial cells (HBMEC) to L. monocytogenes at 4 hr post-infection using oligonucleotide human microarrays. The expression of 456 genes of HBMEC were significantly altered ( p<0.05). Of the 310 induced genes, many were involved in cell cycles, apoptosis/anti-apoptosis, transcription, and defense responses. Among the up-regulated genes, the induced mRNA levels of interleukin-8 and -15 were further confirmed by real-time RT-PCR. Both the cytokines were regarded as potent chemotactic factors for neutrophils, natural killer cells, and T-lymphocytes. These responses suggest that HBMEC is capable of recruiting cells of innate and adaptive immune responses during the early L. monocytogenes infection.
590 $a School code: 0132.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0359
690 $a 0410
710 2 0 $a Mississippi State University. $3 1017550
773 0 $t Dissertation Abstracts International $g 68-07B.
790 $a 0132
790 1 0 $a Wang, Chinling, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3270471