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Identification and stability of anth...

Dee, Stacy.

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Identification and stability of anthocyanins in Plantago lanceolata.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Identification and stability of anthocyanins in Plantago lanceolata./
Author:	Dee, Stacy.
Description:	87 p.
Notes:	Adviser: Nadja B. Cech.
Contained By:	Masters Abstracts International46-01.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1445535
ISBN:	9780549136316

Identification and stability of anthocyanins in Plantago lanceolata.
Dee, Stacy.

Identification and stability of anthocyanins in Plantago lanceolata. - 87 p.

Adviser: Nadja B. Cech.

Thesis (M.S.)--The University of North Carolina at Greensboro, 2007.

Several laboratories have analyzed the content and stability of anthocyanins from foods and flowering plants ranging from potatoes to petunias. Previous research in the Cech and Lacey laboratories tentatively identified anthocyanins in Plantago lanceolata using reversed phase high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS). The goal of this research was to gain more information about the identification of these anthocyanins using hydrolysis and nuclear magnetic resonance (NMR) and to test the stability of these anthocyanins when stored for various periods of time at three different temperatures (room temperature, -20, -80 °C). Repeatability studies were also conducted to validate the analytical method.

ISBN: 9780549136316Subjects--Topical Terms:

586156
Chemistry, Analytical.

Identification and stability of anthocyanins in Plantago lanceolata.
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020 $a 9780549136316
035 $a (UMI)AAI1445535
035 $a AAI1445535
040 $a UMI $c UMI
100 1 $a Dee, Stacy. $3 1291662
245 1 0 $a Identification and stability of anthocyanins in Plantago lanceolata.
300 $a 87 p.
500 $a Adviser: Nadja B. Cech.
500 $a Source: Masters Abstracts International, Volume: 46-01, page: .
502 $a Thesis (M.S.)--The University of North Carolina at Greensboro, 2007.
520 $a Several laboratories have analyzed the content and stability of anthocyanins from foods and flowering plants ranging from potatoes to petunias. Previous research in the Cech and Lacey laboratories tentatively identified anthocyanins in Plantago lanceolata using reversed phase high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS). The goal of this research was to gain more information about the identification of these anthocyanins using hydrolysis and nuclear magnetic resonance (NMR) and to test the stability of these anthocyanins when stored for various periods of time at three different temperatures (room temperature, -20, -80 °C). Repeatability studies were also conducted to validate the analytical method.
520 $a Hydrolysis experiments and analysis by HPLC-ESI-MS confirmed the presence of cyanidin and peonidin glycosides in the original P. lanceolata extract. The most abundant anthocyanin was isolated from P. lanceolata, but attempts to collect an NMR spectrum of this sample were not successful due to the small yield and apparent low purity of the sample isolated. The method variability from injection to injection on the same day was determined to be 10--20%, variability from day to day on the same extract was 30% and the variability from extraction to extraction on different days was 30--60% for cyanidin glycosides a and c, delphinidin glycoside b, peonidin glycoside a, and petunidin glycoside b. Stability studies revealed that there was no degradation that exceeded the variability of the method from day to day in both the -20 and -80°C temperatures for any of the anthocyanins. There was however, significant degradation in the room temperature condition for the cyanidin glycosides a and c.
590 $a School code: 0154.
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0486
710 2 0 $a The University of North Carolina at Greensboro. $b Chemistry and Biochemistry. $3 1291663
773 0 $t Masters Abstracts International $g 46-01.
790 $a 0154
790 1 0 $a Cech, Nadja B., $e advisor
790 1 0 $a Chiu, Norman $e committee member
790 1 0 $a Lacey, Elisibeth $e committee member
791 $a M.S.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1445535