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Fabrication of biosensors for food p...

Ruengruglikit, Chada.

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Fabrication of biosensors for food pathogen detection.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Fabrication of biosensors for food pathogen detection./
Author:	Ruengruglikit, Chada.
Description:	174 p.
Notes:	Adviser: Qingrong Huang.
Contained By:	Dissertation Abstracts International68-02B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3253016

Fabrication of biosensors for food pathogen detection.
Ruengruglikit, Chada.

Fabrication of biosensors for food pathogen detection. - 174 p.

Adviser: Qingrong Huang.

Thesis (Ph.D.)--Rutgers The State University of New Jersey - New Brunswick, 2007.

Food safety and biosecurity are among the imminent concerns nationwide nowadays. To ensure food safety and biosecurity of the nation, novel systems are required for the fast, reliable, and highly sensitive detection of biological agents in foods and water. The overall theme of this dissertation is develop new substrate materials, new immobilization methods for biological probes, and new sensing techniques for the next generation biosensors.Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Fabrication of biosensors for food pathogen detection.
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035 $a (UMI)AAI3253016
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040 $a UMI $c UMI
100 1 $a Ruengruglikit, Chada. $3 1291602
245 1 0 $a Fabrication of biosensors for food pathogen detection.
300 $a 174 p.
500 $a Adviser: Qingrong Huang.
500 $a Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0683.
502 $a Thesis (Ph.D.)--Rutgers The State University of New Jersey - New Brunswick, 2007.
520 $a Food safety and biosecurity are among the imminent concerns nationwide nowadays. To ensure food safety and biosecurity of the nation, novel systems are required for the fast, reliable, and highly sensitive detection of biological agents in foods and water. The overall theme of this dissertation is develop new substrate materials, new immobilization methods for biological probes, and new sensing techniques for the next generation biosensors.
520 $a First, nanoporous methyl silsesquioxane (MSSQ) was produced via the thermosetting of MSSQ, templated by thermally decomposable pore generators (porogens) such as pluoronic surfactant P123 and polymeric nanoparticles. Oligonucleotide microarrays prepared by UV/ozone photolithography show selective adsorption only to the hydrophilic surface, and the oligonulceotide probes maintain high specificity. The highly porous patterned MSSQ films increase fluorescence signals in DNA microarrays by at least six folds compared with flat MSSQ films. Second, microcontact printing (MCP) has been used to fabricate antibody-based microarrays through the selective passivation of pre-patterned PEG on aldehyde-activated glass surface. Patterned antibodies still maintain their activity and are capable of identifying pathogenic cells through antibody-cell bioaffinity. The detection limit of 0.1 cell/ml is achieved for the applied cell solution. Third, a novel immunosensor based on quartz crystal microbalance with dissipation monitoring has been developed to detect cholera toxin (CT) in real time through the incorporation of direct immunoassay, where anti-CT antibody is chemically immobilized on the surface of self-assemble monolayer of mercaptoundecanoic acid (11-MUA)-modified quartz crystal electrode. The adsorption of CT on the anti-CT antibody surface is also monitored by tapping mode atomic force microscopy. The influence of antibody probe density, orientation of antibody, and detection environment on the detection sensitivity and binding characteristics of CT to antibody has also been investigated. Our results show that in the phosphate buffer saline (PBS) of pH=7.3, the detection limit of CT can be reached at CT concentration as low as 0.05 mug/ml (or 0.6 nM).
590 $a School code: 0190.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
690 $a 0359
710 2 0 $a Rutgers The State University of New Jersey - New Brunswick. $3 1017590
773 0 $t Dissertation Abstracts International $g 68-02B.
790 $a 0190
790 1 0 $a Huang, Qingrong, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3253016