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Direct detection of Staphylococcus a...

Ivey, James C.

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Direct detection of Staphylococcus aureus using rapid cycle real time polymerase chain reaction and its effect on patient isolation stays and antibiotic usage in a community hospital.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Direct detection of Staphylococcus aureus using rapid cycle real time polymerase chain reaction and its effect on patient isolation stays and antibiotic usage in a community hospital./
Author:	Ivey, James C.
Description:	108 p.
Notes:	Adviser: Gary Stein.
Contained By:	Dissertation Abstracts International68-01B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3249469

Direct detection of Staphylococcus aureus using rapid cycle real time polymerase chain reaction and its effect on patient isolation stays and antibiotic usage in a community hospital.
Ivey, James C.

Direct detection of Staphylococcus aureus using rapid cycle real time polymerase chain reaction and its effect on patient isolation stays and antibiotic usage in a community hospital. - 108 p.

Adviser: Gary Stein.

Thesis (Ph.D.)--Touro University International, 2007.

The purpose of the study was to test a rapid polymerase chain reaction (PCR) assay for MRSA on a variety of body sites and investigate effects of providing rapid results on infection control practices and antibiotic usage in the hospital environment.Subjects--Topical Terms:

1017734
Biology, Microbiology.

Direct detection of Staphylococcus aureus using rapid cycle real time polymerase chain reaction and its effect on patient isolation stays and antibiotic usage in a community hospital.
LDR:02540nam 2200301 a 45 001 967731
005 20110915
008 110915s2007 eng d
035 $a (UMI)AAI3249469
035 $a AAI3249469
040 $a UMI $c UMI
100 1 $a Ivey, James C. $3 1291599
245 1 0 $a Direct detection of Staphylococcus aureus using rapid cycle real time polymerase chain reaction and its effect on patient isolation stays and antibiotic usage in a community hospital.
300 $a 108 p.
500 $a Adviser: Gary Stein.
500 $a Source: Dissertation Abstracts International, Volume: 68-01, Section: B, page: 0091.
502 $a Thesis (Ph.D.)--Touro University International, 2007.
520 $a The purpose of the study was to test a rapid polymerase chain reaction (PCR) assay for MRSA on a variety of body sites and investigate effects of providing rapid results on infection control practices and antibiotic usage in the hospital environment.
520 $a Samples from multiple body sites from 201 patients suspected of MRSA colonization were analyzed by both rapid PCR and conventional MRSA culture. PCR results and MRSA culture results were compared and the PCR test was then used to test patients in the 75 sample study group. Test turnaround time, patient age, gender, antibiotic usage, days in isolation, and diagnoses were collected on 75 patients included in the control group before PCR was initiated and 75 patients included in the study group after PCR was initiated.
520 $a Results showed that real time PCR methodology is suitable for a variety of specimen types and has sensitivity equivalent to MRSA culture. Turnaround times were significantly lower for PCR (m = 18.15, sd = 2.03) than for Culture (m = 45.30, sd = 2.05) hours. The number of days in isolation was lower for the study group (m = 3.34, sd = 2.79 days) than for the control group (m = 4.36, sd = 2.68). The PCR group (m = 2.35, sd = 1.65) required fewer vancomycin doses than the control group (m = 3.58, sd = 1.88). A repeated measures ANOVA was used to determine if changes were occurring in the number of nosocomial MRSA isolates. The results were found to be statistically significant, indicating a decrease in the number of isolates from 2004 and 2005 to 2006.
590 $a School code: 1421.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Health Sciences, Medicine and Surgery. $3 1017756
690 $a 0307
690 $a 0410
690 $a 0564
710 2 0 $a Touro University International. $3 1023252
773 0 $t Dissertation Abstracts International $g 68-01B.
790 $a 1421
790 1 0 $a Stein, Gary, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3249469