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Electrospun linear polyethyleneimine...

Hundt, Nadia Khanam.

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Electrospun linear polyethyleneimine scaffolds for cell growth.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Electrospun linear polyethyleneimine scaffolds for cell growth./
Author:	Hundt, Nadia Khanam.
Description:	54 p.
Notes:	Adviser: Kenneth J. Balkus, Jr.
Contained By:	Masters Abstracts International45-01.
Subject:	Chemistry, Polymer. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1437210
ISBN:	9780542804922

Electrospun linear polyethyleneimine scaffolds for cell growth.
Hundt, Nadia Khanam.

Electrospun linear polyethyleneimine scaffolds for cell growth. - 54 p.

Adviser: Kenneth J. Balkus, Jr.

Thesis (M.S.)--The University of Texas at Dallas, 2006.

Unique biocompatible scaffolds were produced by electrospinning crosslinked linear polyethyleneimine (PEI) with succinic anhydride and 1, 4-butanediol diglycidyl ether. Nonwoven mats of PEI fibers in the range of 1600 nm to 687 nm were evaluated as interaction scaffolds for normal human fibroblast (NHF) cells. The electrospun scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), UV-VIS spectrophotometer, and thermal gravimetric analysis (TGA). The growth of the NHF cells was followed by Field Emission Scanning Electron (SEM), optical and fluorescence microscopies. Cell viability was evaluated by staining with propidium iodide for dead cells and fluorescein diacetate for live cells. Immunofluorescence with fixed cells on the scaffolds was examined by staining the endoplasmic reticulum with Rabbit anti GRP 78/Alexa 488 Goat anti Rabbit and staining the nuclei with 4'-6'-Diamidino-2-phenylindole (DAPI). Fluorescence studies confirmed that NHF cells attached and spread through out the crosslinked linear polyethyleneimine scaffold. The attachment and spreading of cells suggests electrospun linear polyethyleneimine scaffold support growth of normal human fibroblasts cells. Thus, these biomaterial scaffolds may be useful in tissue engineering.

ISBN: 9780542804922Subjects--Topical Terms:

1018428
Chemistry, Polymer.

Electrospun linear polyethyleneimine scaffolds for cell growth.
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005 20110915
008 110915s2006 eng d
020 $a 9780542804922
035 $a (UMI)AAI1437210
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100 1 $a Hundt, Nadia Khanam. $3 1291335
245 1 0 $a Electrospun linear polyethyleneimine scaffolds for cell growth.
300 $a 54 p.
500 $a Adviser: Kenneth J. Balkus, Jr.
500 $a Source: Masters Abstracts International, Volume: 45-01, page: 0331.
502 $a Thesis (M.S.)--The University of Texas at Dallas, 2006.
520 $a Unique biocompatible scaffolds were produced by electrospinning crosslinked linear polyethyleneimine (PEI) with succinic anhydride and 1, 4-butanediol diglycidyl ether. Nonwoven mats of PEI fibers in the range of 1600 nm to 687 nm were evaluated as interaction scaffolds for normal human fibroblast (NHF) cells. The electrospun scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), UV-VIS spectrophotometer, and thermal gravimetric analysis (TGA). The growth of the NHF cells was followed by Field Emission Scanning Electron (SEM), optical and fluorescence microscopies. Cell viability was evaluated by staining with propidium iodide for dead cells and fluorescein diacetate for live cells. Immunofluorescence with fixed cells on the scaffolds was examined by staining the endoplasmic reticulum with Rabbit anti GRP 78/Alexa 488 Goat anti Rabbit and staining the nuclei with 4'-6'-Diamidino-2-phenylindole (DAPI). Fluorescence studies confirmed that NHF cells attached and spread through out the crosslinked linear polyethyleneimine scaffold. The attachment and spreading of cells suggests electrospun linear polyethyleneimine scaffold support growth of normal human fibroblasts cells. Thus, these biomaterial scaffolds may be useful in tissue engineering.
590 $a School code: 0382.
650 4 $a Chemistry, Polymer. $3 1018428
690 $a 0495
710 2 0 $a The University of Texas at Dallas. $3 1018411
773 0 $t Masters Abstracts International $g 45-01.
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790 1 0 $a Balkus, Kenneth J., Jr., $e advisor
791 $a M.S.
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