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Evolution of altered signaling in th...

Shumway, Laurence Alan.

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Evolution of altered signaling in the yeast Saccharomyces cerevisiae.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Evolution of altered signaling in the yeast Saccharomyces cerevisiae./
Author:	Shumway, Laurence Alan.
Description:	187 p.
Notes:	Adviser: Andrew Wood Murray.
Contained By:	Dissertation Abstracts International68-02B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3251315

Evolution of altered signaling in the yeast Saccharomyces cerevisiae.
Shumway, Laurence Alan.

Evolution of altered signaling in the yeast Saccharomyces cerevisiae. - 187 p.

Adviser: Andrew Wood Murray.

Thesis (Ph.D.)--Harvard University, 2007.

In this dissertation I will present the evolution and characterization of a genetically complex trait. The trait is defined by fluctuations in a fluorescent FUS1 transcriptional reporter. The trait was evolved by an iterated, alternating selection scheme; cells were alternately selected to have either high or low fluorescence. The fluctuations occur in the absence of external stimulus.Subjects--Topical Terms:

1017730
Biology, Genetics.

Evolution of altered signaling in the yeast Saccharomyces cerevisiae.
LDR:02182nam 2200289 a 45 001 967438
005 20110915
008 110915s2007 eng d
035 $a (UMI)AAI3251315
035 $a AAI3251315
040 $a UMI $c UMI
100 1 $a Shumway, Laurence Alan. $3 1291314
245 1 0 $a Evolution of altered signaling in the yeast Saccharomyces cerevisiae.
300 $a 187 p.
500 $a Adviser: Andrew Wood Murray.
500 $a Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0834.
502 $a Thesis (Ph.D.)--Harvard University, 2007.
520 $a In this dissertation I will present the evolution and characterization of a genetically complex trait. The trait is defined by fluctuations in a fluorescent FUS1 transcriptional reporter. The trait was evolved by an iterated, alternating selection scheme; cells were alternately selected to have either high or low fluorescence. The fluctuations occur in the absence of external stimulus.
520 $a This reporter is a proxy for activation of the pheromone response pathway in wild-type Saccharomyces cerevisiae. Key components of the pheromone response pathway including the pheromone receptor, its associated trimeric G-protein, a scaffolding protein, and effector kinase, are non-essential for the trait. The components of the Cdc42-dependent invasive growth MAPK cascade are essential for the fluctuations. Four mutations of strong effect instruct this trait, two of which were identified in this study. A third mutation can be effectively phenocopied by deletion of key components of the osmotolerance pathway. Our model proposes that the mutations support a positive signal that stimulates the invasive growth pathway. One of the identified mutations, a loss of function mutation in an invasive growth transcription co-factor, TEC1, permits this signal to be directed to a reporter for the mating pathway.
590 $a School code: 0084.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biophysics, General. $3 1019105
690 $a 0369
690 $a 0410
690 $a 0786
710 2 0 $a Harvard University. $3 528741
773 0 $t Dissertation Abstracts International $g 68-02B.
790 $a 0084
790 1 0 $a Murray, Andrew Wood, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3251315