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Effect of prebiotic oligosaccharides...

Shoaf, Kari.

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Effect of prebiotic oligosaccharides on enteropathogenic Escherichia coli adherence.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Effect of prebiotic oligosaccharides on enteropathogenic Escherichia coli adherence./
Author:	Shoaf, Kari.
Description:	179 p.
Notes:	Adviser: Robert W. Hutkins.
Contained By:	Dissertation Abstracts International67-11B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3243741
ISBN:	9780542994128

Effect of prebiotic oligosaccharides on enteropathogenic Escherichia coli adherence.
Shoaf, Kari.

Effect of prebiotic oligosaccharides on enteropathogenic Escherichia coli adherence. - 179 p.

Adviser: Robert W. Hutkins.

Thesis (Ph.D.)--The University of Nebraska - Lincoln, 2007.

Antiadhesion therapy has been found to be a very promising means to prevent and treat infections in the respiratory, urinary, and gastrointestinal tract. Despite the considerable research interest in antiadhesives, relatively little is known about the ability of commercial dietary oligosaccharides, such as prebiotics, to inhibit pathogen adherence. The goal of this research was to determine if commercially available prebiotic oligosaccharides could function as antiadhesive agents against enteropathogenic E. coli (EPEC).

ISBN: 9780542994128Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Effect of prebiotic oligosaccharides on enteropathogenic Escherichia coli adherence.
LDR:03245nam 2200313 a 45 001 964091
005 20110901
008 110901s2007 eng d
020 $a 9780542994128
035 $a (UMI)AAI3243741
035 $a AAI3243741
040 $a UMI $c UMI
100 1 $a Shoaf, Kari. $3 1287162
245 1 0 $a Effect of prebiotic oligosaccharides on enteropathogenic Escherichia coli adherence.
300 $a 179 p.
500 $a Adviser: Robert W. Hutkins.
500 $a Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6129.
502 $a Thesis (Ph.D.)--The University of Nebraska - Lincoln, 2007.
520 $a Antiadhesion therapy has been found to be a very promising means to prevent and treat infections in the respiratory, urinary, and gastrointestinal tract. Despite the considerable research interest in antiadhesives, relatively little is known about the ability of commercial dietary oligosaccharides, such as prebiotics, to inhibit pathogen adherence. The goal of this research was to determine if commercially available prebiotic oligosaccharides could function as antiadhesive agents against enteropathogenic E. coli (EPEC).
520 $a To investigate the ability of prebiotics to inhibit EPEC adherence, inhibition assays were performed by comparing adherence rates of EPEC on HEp-2 and Caco-2 cells in the presence of galactooligosaccharides (GOS), inulin, fructooligosaccharides, lactulose, or raffinose. These data showed that GOS significantly reduced binding of EPEC more than the other prebiotics tested. In addition, binding inhibition by GOS was shown to be dose-dependent and saturable at 16 mg/ml. Microscopic analyses indicated that GOS also reduced the number of EPEC per microcolony and the total number of microcolonies per infected cell.
520 $a Both therapeutic and prophylactic GOS treatments were also examined. GOS was unable to displace previously adhered EPEC, but could prevent EPEC adherence when administered prior to infection. Additionally, GOS did not affect EPEC autoaggregation. To determine if GOS was inhibiting adherence at a molecular level, the expression of BfpA, a bundle forming pili protein involved in localized adherence, was examined. GOS did not affect BfpA expression indicating that adherence inhibition was not due to the absence of this adhesin.
520 $a To further examine the role of EPEC adhesins in GOS-mediated adherence inhibition, adherence assays using HEp-2 and Caco-2 cell lines were performed using isogenic EPEC mutants. In general, GOS did not affect the adherence of strains lacking BfpA, suggesting that GOS may interfere specifically with BfpA-receptor interactions rather than with other potential EPEC adhesins and their receptors. These results show that commercial prebiotics, particularly GOS, directly inhibit the adherence of E. coli, and provides evidence that these agents may be used as antiadhesives against pathogens in both humans and animals.
590 $a School code: 0138.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0359
690 $a 0410
710 2 0 $a The University of Nebraska - Lincoln. $3 1024939
773 0 $t Dissertation Abstracts International $g 67-11B.
790 $a 0138
790 1 0 $a Hutkins, Robert W., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3243741