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Analytical potential of polymerized ...

Santos, Marina.

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Analytical potential of polymerized liposomes bound to lanthanide ions for qualitative and quantitative analysis of proteins.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Analytical potential of polymerized liposomes bound to lanthanide ions for qualitative and quantitative analysis of proteins./
Author:	Santos, Marina.
Description:	177 p.
Notes:	Adviser: Andres Campiglia.
Contained By:	Dissertation Abstracts International68-02B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3254313

Analytical potential of polymerized liposomes bound to lanthanide ions for qualitative and quantitative analysis of proteins.
Santos, Marina.

Analytical potential of polymerized liposomes bound to lanthanide ions for qualitative and quantitative analysis of proteins. - 177 p.

Adviser: Andres Campiglia.

Thesis (Ph.D.)--University of Central Florida, 2006.

One of the intriguing features of biological systems is the prevalence of highly selective and often very strong interactions among different cellular components. Such interactions play a variety of organizational, mechanical, and physiological roles at the cellular and organism levels. Antigen-antibody complexes are representative examples of highly selective and potent interactions involving proteins. The marked specificity of protein-antibody complexes have led to a wide range of applications in cellular and molecular biology related research. They have become an integral research tool in the present genomic and proteomic era. Unfortunately, the production of selective tools based on antigen-antibody interactions requires cumbersome protocols.Subjects--Topical Terms:

586156
Chemistry, Analytical.

Analytical potential of polymerized liposomes bound to lanthanide ions for qualitative and quantitative analysis of proteins.
LDR:03651nam 2200289 a 45 001 958527
005 20110704
008 110704s2006 eng d
035 $a (UMI)AAI3254313
035 $a AAI3254313
040 $a UMI $c UMI
100 1 $a Santos, Marina. $3 1281988
245 1 0 $a Analytical potential of polymerized liposomes bound to lanthanide ions for qualitative and quantitative analysis of proteins.
300 $a 177 p.
500 $a Adviser: Andres Campiglia.
500 $a Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0941.
502 $a Thesis (Ph.D.)--University of Central Florida, 2006.
520 $a One of the intriguing features of biological systems is the prevalence of highly selective and often very strong interactions among different cellular components. Such interactions play a variety of organizational, mechanical, and physiological roles at the cellular and organism levels. Antigen-antibody complexes are representative examples of highly selective and potent interactions involving proteins. The marked specificity of protein-antibody complexes have led to a wide range of applications in cellular and molecular biology related research. They have become an integral research tool in the present genomic and proteomic era. Unfortunately, the production of selective tools based on antigen-antibody interactions requires cumbersome protocols.
520 $a The long term goal of this project explores the possibility of manipulating liposomes to serve as the chemical receptors ("artificial antibodies") against selected proteins. Cellular lipids (e.g., lipid rafts) are known to facilitate highly selective binding of proteins on cell membranes. The binding of proteins to cell membranes can be envisaged to be modulated via interactions between polar (charged) and non-polar head groups of lipids and the complementary amino acid residues of proteins. Their interaction is facilitated by a combination of van der Waals, electrostatic, hydrogen bonding and hydrophobic forces. A further interesting aspect of the above interaction is the "fluidity" of the membrane resident lipids, which can migrate from other regions to further enhance the complementary interactions of proteins on the initially "docked" membrane surface. With these features in mind, the end goal of this project is expected to deliver lipid-based chemical receptors "synthetically" designed against proteins to function as "artificial antibodies". Protein sensing will be accomplished with lipid receptors assembled in templated polymerized liposomes.
520 $a The research presented here specifically focus on the analytical aspects of protein sensing via polymerized liposome vesicles. Lanthanide ions (Eu 3+ and Tb3+) are incorporated into polymerized liposome with the expectation to "report" quantitative and qualitative information on the interacting protein. Our proposition is to extract quantitative and qualitative information from the luminescence intensity and the luminescence lifetime of the lanthanide ion, respectively. A thorough investigation is presented regarding the analytical potential of these two parameters for protein sensing. Two chemometic approaches - namely partial least squares (PLS-1) and artificial neural networks (ANN) - are compared towards quantitative and qualitative analysis of proteins in binary mixtures.
590 $a School code: 0705.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0486
690 $a 0487
710 2 0 $a University of Central Florida. $3 1018467
773 0 $t Dissertation Abstracts International $g 68-02B.
790 $a 0705
790 1 0 $a Campiglia, Andres, $e advisor
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3254313