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T cell interactions in the foreign b...

Rodriguez, Analiz.

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T cell interactions in the foreign body response to biomaterials.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	T cell interactions in the foreign body response to biomaterials./
Author:	Rodriguez, Analiz.
Description:	194 p.
Notes:	Adviser: James M. Anderson.
Contained By:	Dissertation Abstracts International68-10B.
Subject:	Engineering, Biomedical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3286200
ISBN:	9780549291008

T cell interactions in the foreign body response to biomaterials.
Rodriguez, Analiz.

T cell interactions in the foreign body response to biomaterials. - 194 p.

Adviser: James M. Anderson.

Thesis (Ph.D.)--Case Western Reserve University, 2008.

The role of T cells in the foreign body response to biomaterials has not been elucidated. Clinically, however patients implanted with a left ventricular assist device, a polyurethane, exhibit biomaterial induced T cell activation. Our laboratory has demonstrated that lymphocytes enhance macrophage adhesion and fusion in vitro, although it is not known if these effects are mediated by T cells. The goal of this research was to investigate potential T cell interactions in the foreign body response to three clinically relevant non-biodegradable synthetic biomedical polymers: Elasthane 80A (PEU), silicone rubber (SR), and polyethylene terephthalate (PET). Chapter 2 addresses the role that adaptive immunity, specifically memory, may play by evaluating the primary and secondary host response. We observed quantitative increases in T cells following secondary biomaterial exposure without alterations in T cell subset distributions. In Chapter 3, further characterization of the biomaterial implant site was carried out by analysis of cytokine profiles. The presence of a polymer implant did affect cytokine profiles. Most notably, IL-6 and TNFalpha levels were significantly greater in those animals implanted with PEU and SR, materials that do not promote fusion. In Chapter 4, in vitro T cell activation was investigated using human blood mononuclear cells and assessed by upregulation of cell surface activation markers, proliferation, and IL-2 production. T cell activation was not induced in response to the three biomaterials. Therefore in Chapter 5, biomaterials were implanted in T cell deficient mice to determine whether T cells are required in the foreign body response in vivo. This study showed that there are pathways that do not require thymus-matured T lymphocytes which lead to a normal foreign body response to biomaterials in a murine model. Finally in Chapter 6, the role of IL-4 and IL-13 in foreign body giant cell formation in vivo was evaluated and IL-4 was found to be the main fusion inducing cytokine at the biomaterial implant site. This research further characterized the foreign body response in vivo, demonstrated that T cells are not activated in response to these synthetic biomaterials and the foreign body response can progress normally without thymus-matured T cells.

ISBN: 9780549291008Subjects--Topical Terms:

1017684
Engineering, Biomedical.

T cell interactions in the foreign body response to biomaterials.
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005 20110630
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020 $a 9780549291008
035 $a (UMI)AAI3286200
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040 $a UMI $c UMI
100 1 $a Rodriguez, Analiz. $3 1280988
245 1 0 $a T cell interactions in the foreign body response to biomaterials.
300 $a 194 p.
500 $a Adviser: James M. Anderson.
500 $a Source: Dissertation Abstracts International, Volume: 68-10, Section: B, page: 6577.
502 $a Thesis (Ph.D.)--Case Western Reserve University, 2008.
520 $a The role of T cells in the foreign body response to biomaterials has not been elucidated. Clinically, however patients implanted with a left ventricular assist device, a polyurethane, exhibit biomaterial induced T cell activation. Our laboratory has demonstrated that lymphocytes enhance macrophage adhesion and fusion in vitro, although it is not known if these effects are mediated by T cells. The goal of this research was to investigate potential T cell interactions in the foreign body response to three clinically relevant non-biodegradable synthetic biomedical polymers: Elasthane 80A (PEU), silicone rubber (SR), and polyethylene terephthalate (PET). Chapter 2 addresses the role that adaptive immunity, specifically memory, may play by evaluating the primary and secondary host response. We observed quantitative increases in T cells following secondary biomaterial exposure without alterations in T cell subset distributions. In Chapter 3, further characterization of the biomaterial implant site was carried out by analysis of cytokine profiles. The presence of a polymer implant did affect cytokine profiles. Most notably, IL-6 and TNFalpha levels were significantly greater in those animals implanted with PEU and SR, materials that do not promote fusion. In Chapter 4, in vitro T cell activation was investigated using human blood mononuclear cells and assessed by upregulation of cell surface activation markers, proliferation, and IL-2 production. T cell activation was not induced in response to the three biomaterials. Therefore in Chapter 5, biomaterials were implanted in T cell deficient mice to determine whether T cells are required in the foreign body response in vivo. This study showed that there are pathways that do not require thymus-matured T lymphocytes which lead to a normal foreign body response to biomaterials in a murine model. Finally in Chapter 6, the role of IL-4 and IL-13 in foreign body giant cell formation in vivo was evaluated and IL-4 was found to be the main fusion inducing cytokine at the biomaterial implant site. This research further characterized the foreign body response in vivo, demonstrated that T cells are not activated in response to these synthetic biomaterials and the foreign body response can progress normally without thymus-matured T cells.
590 $a School code: 0042.
650 4 $a Engineering, Biomedical. $3 1017684
650 4 $a Health Sciences, Immunology. $3 1017716
690 $a 0541
690 $a 0982
710 2 $a Case Western Reserve University. $3 1017714
773 0 $t Dissertation Abstracts International $g 68-10B.
790 $a 0042
790 1 0 $a Anderson, James M., $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3286200