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Development and application of multi...

Boddeda, Anuradha.

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Development and application of multiple polymerase chain reaction assays to detect ochratoxin-producing Aspergillus and Penicillium, and trichothecene-producing Fusarium in stored and processed grain.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Development and application of multiple polymerase chain reaction assays to detect ochratoxin-producing Aspergillus and Penicillium, and trichothecene-producing Fusarium in stored and processed grain./
Author:	Boddeda, Anuradha.
Description:	173 p.
Notes:	Adviser: Charlene Wolf-Hall.
Contained By:	Dissertation Abstracts International69-03B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3308544
ISBN:	9780549552352

Development and application of multiple polymerase chain reaction assays to detect ochratoxin-producing Aspergillus and Penicillium, and trichothecene-producing Fusarium in stored and processed grain.
Boddeda, Anuradha.

Development and application of multiple polymerase chain reaction assays to detect ochratoxin-producing Aspergillus and Penicillium, and trichothecene-producing Fusarium in stored and processed grain. - 173 p.

Adviser: Charlene Wolf-Hall.

Thesis (Ph.D.)--North Dakota State University, 2008.

Cereal crop plants and stored grains are colonized by many mycotoxigenic fungal species such as Aspergillus ochraceus and Penicillium verrucosum, which produce ochratoxins, and Fusarium graminearum, which produces trichothecenes. Polymerase chain reaction based assays were developed and used to detect and quantify these mycotoxin-producing fungi in stored and processed barley. The multiplex real-time PCR method developed in the present study can simultaneously detect ochratoxin-producing A. ochraceus and P. verrucosum and trichothecene-producing F. graminearum. It can also quantify genomic DNA over five orders of magnitude (3 pg to 30 ng). We also observed that the biomass and volatile content of these fungi increased in barley stored at various moisture contents (13%, 18%, 20%, and 25%) over time. The volatiles, 3-methyl-1-butanol, 1-octen-3-ol and 3-octanone, significantly (P <0.05) increased, reaching as high as 5.7 microg/g, 14.0 microg/g and 12.3 microg/g in naturally infected barley on day 22 of storage at 25% moisture content. We observed that the mycotoxin-producing gene (Tri5) expression occurred during the third day of the germination stage of barley malting. We found, by random polymorphic DNA analysis that electron beam irradiation may have caused mutations in F. graminearum and that the mycotoxicity of the fungus was reduced by irradiation.

ISBN: 9780549552352Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Development and application of multiple polymerase chain reaction assays to detect ochratoxin-producing Aspergillus and Penicillium, and trichothecene-producing Fusarium in stored and processed grain.
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100 1 $a Boddeda, Anuradha. $3 1280879
245 1 0 $a Development and application of multiple polymerase chain reaction assays to detect ochratoxin-producing Aspergillus and Penicillium, and trichothecene-producing Fusarium in stored and processed grain.
300 $a 173 p.
500 $a Adviser: Charlene Wolf-Hall.
500 $a Source: Dissertation Abstracts International, Volume: 69-03, Section: B, page: 1388.
502 $a Thesis (Ph.D.)--North Dakota State University, 2008.
520 $a Cereal crop plants and stored grains are colonized by many mycotoxigenic fungal species such as Aspergillus ochraceus and Penicillium verrucosum, which produce ochratoxins, and Fusarium graminearum, which produces trichothecenes. Polymerase chain reaction based assays were developed and used to detect and quantify these mycotoxin-producing fungi in stored and processed barley. The multiplex real-time PCR method developed in the present study can simultaneously detect ochratoxin-producing A. ochraceus and P. verrucosum and trichothecene-producing F. graminearum. It can also quantify genomic DNA over five orders of magnitude (3 pg to 30 ng). We also observed that the biomass and volatile content of these fungi increased in barley stored at various moisture contents (13%, 18%, 20%, and 25%) over time. The volatiles, 3-methyl-1-butanol, 1-octen-3-ol and 3-octanone, significantly (P <0.05) increased, reaching as high as 5.7 microg/g, 14.0 microg/g and 12.3 microg/g in naturally infected barley on day 22 of storage at 25% moisture content. We observed that the mycotoxin-producing gene (Tri5) expression occurred during the third day of the germination stage of barley malting. We found, by random polymorphic DNA analysis that electron beam irradiation may have caused mutations in F. graminearum and that the mycotoxicity of the fungus was reduced by irradiation.
590 $a School code: 0157.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
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710 2 $a North Dakota State University. $3 1021724
773 0 $t Dissertation Abstracts International $g 69-03B.
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790 1 0 $a Wolf-Hall, Charlene, $e advisor
791 $a Ph.D.
792 $a 2008
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