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Cell wall biosynthesis in Zea mays.

van Erp, Henricus EG.

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Cell wall biosynthesis in Zea mays.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Cell wall biosynthesis in Zea mays./
Author:	van Erp, Henricus EG.
Description:	157 p.
Notes:	Adviser: Jonathan D. Walton.
Contained By:	Dissertation Abstracts International69-01B.
Subject:	Biology, Plant Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3298122
ISBN:	9780549421993

Cell wall biosynthesis in Zea mays.
van Erp, Henricus EG.

Cell wall biosynthesis in Zea mays. - 157 p.

Adviser: Jonathan D. Walton.

Thesis (Ph.D.)--Michigan State University, 2007.

For my thesis research I studied dark-grown maize seedlings as a model system for cell wall biosynthesis. When maize seedlings are grown in complete darkness an organ develops called the mesocotyl, which serves to position the seedling towards the light. After exposure to light, the rapid elongation of the mesocotyl is reduced and concomitant with this, certain enzymatic activities related to cell wall biosynthesis are down-regulated, including a Golgi-localized glucan synthase. I attempted to identify the glucan synthase with biochemical methods and characterized the product it synthesizes. I established that it synthesizes (1,4)-beta-glucan. Because pure (1,4)-beta-glucan is not known to be synthesized in the Golgi, the glucan synthase might be involved in the synthesis of other polysaccharides, such as xyloglucan or mixed-linkage glucan (MLG). The glucan synthase was successfully solubilized with digitonin and it still synthesized (1,4)-beta-glucan. However, after chromatography glucose-6-phosphate and glucose-1,6-diphosphate were synthesized instead of (1,4)-beta-glucan.

ISBN: 9780549421993Subjects--Topical Terms:

1017865
Biology, Plant Physiology.

Cell wall biosynthesis in Zea mays.
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020 $a 9780549421993
035 $a (UMI)AAI3298122
035 $a AAI3298122
040 $a UMI $c UMI
100 1 $a van Erp, Henricus EG. $3 1277316
245 1 0 $a Cell wall biosynthesis in Zea mays.
300 $a 157 p.
500 $a Adviser: Jonathan D. Walton.
500 $a Source: Dissertation Abstracts International, Volume: 69-01, Section: B, page: 0156.
502 $a Thesis (Ph.D.)--Michigan State University, 2007.
520 $a For my thesis research I studied dark-grown maize seedlings as a model system for cell wall biosynthesis. When maize seedlings are grown in complete darkness an organ develops called the mesocotyl, which serves to position the seedling towards the light. After exposure to light, the rapid elongation of the mesocotyl is reduced and concomitant with this, certain enzymatic activities related to cell wall biosynthesis are down-regulated, including a Golgi-localized glucan synthase. I attempted to identify the glucan synthase with biochemical methods and characterized the product it synthesizes. I established that it synthesizes (1,4)-beta-glucan. Because pure (1,4)-beta-glucan is not known to be synthesized in the Golgi, the glucan synthase might be involved in the synthesis of other polysaccharides, such as xyloglucan or mixed-linkage glucan (MLG). The glucan synthase was successfully solubilized with digitonin and it still synthesized (1,4)-beta-glucan. However, after chromatography glucose-6-phosphate and glucose-1,6-diphosphate were synthesized instead of (1,4)-beta-glucan.
520 $a I studied the correlation of the light-regulation of cell wall biosynthetic enzymatic activities to transcript levels of the maize Cellulose Synthase Like (CSL) gene family. The CSL gene family encodes the enzymes involved in the synthesis of the (1,4)-beta-linked glycan backbones of hemicelluloses such as xyloglucan, MLG and (1,4)-beta-mannan. As a first step, I annotated the maize CSL gene family. The rice and maize CSL gene families are similar, except for a few differences.
520 $a The CSLC gene family is expanded from five genes in Arabidopsis and six in rice to potentially twelve in maize. Also, an atypical CSL gene was found called CSLX. The CSLX protein is closely related to the CSLG proteins from poplar, which suggests that the CSLX protein belongs to the CSLG subfamily. Assays for enzymatic activities related to cell wall biosynthesis such as callose synthase, xylan synthase, glucan synthase, mannan synthase, UDP-galactose incorporating activity and latent inosine diphosphatase (IDPase) were performed. Mannan synthase and UDP-galactose incorporation were strongly reduced by light-treatment. Some of the CSLA genes encode for mannan synthases. The reduction in mannan synthase activity correlated with the reduction in CSLA transcript levels after light-treatment. This suggests that mannan biosynthesis is regulated at the level of transcription.
590 $a School code: 0128.
650 4 $a Biology, Plant Physiology. $3 1017865
690 $a 0817
710 2 $a Michigan State University. $3 676168
773 0 $t Dissertation Abstracts International $g 69-01B.
790 $a 0128
790 1 0 $a Walton, Jonathan D., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3298122