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The effect of prostaglandin F2alpha ...

Gonzales, Elizabeth.

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The effect of prostaglandin F2alpha and 15-keto-prostaglandin F2alpha on adipocyte leptin gene expression during different stages of the estrous cycle in heifers.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The effect of prostaglandin F2alpha and 15-keto-prostaglandin F2alpha on adipocyte leptin gene expression during different stages of the estrous cycle in heifers./
Author:	Gonzales, Elizabeth.
Description:	50 p.
Notes:	Adviser: Michelle R. Garcia.
Contained By:	Masters Abstracts International46-01.
Subject:	Agriculture, Animal Culture and Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1445786
ISBN:	9780549147565

The effect of prostaglandin F2alpha and 15-keto-prostaglandin F2alpha on adipocyte leptin gene expression during different stages of the estrous cycle in heifers.
Gonzales, Elizabeth.

The effect of prostaglandin F2alpha and 15-keto-prostaglandin F2alpha on adipocyte leptin gene expression during different stages of the estrous cycle in heifers. - 50 p.

Adviser: Michelle R. Garcia.

Thesis (M.S.)--Texas A&M University - Kingsville, 2007.

Key words. leptin, Pgf2alpha, 15-keto-Pgf2alpha, heifers

ISBN: 9780549147565Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

The effect of prostaglandin F2alpha and 15-keto-prostaglandin F2alpha on adipocyte leptin gene expression during different stages of the estrous cycle in heifers.
LDR:03053nam 2200289 a 45 001 949244
005 20110525
008 110525s2007 ||||||||||||||||| ||eng d
020 $a 9780549147565
035 $a (UMI)AAI1445786
035 $a AAI1445786
040 $a UMI $c UMI
100 1 $a Gonzales, Elizabeth. $3 1272622
245 1 4 $a The effect of prostaglandin F2alpha and 15-keto-prostaglandin F2alpha on adipocyte leptin gene expression during different stages of the estrous cycle in heifers.
300 $a 50 p.
500 $a Adviser: Michelle R. Garcia.
500 $a Source: Masters Abstracts International, Volume: 46-01, page: 0213.
502 $a Thesis (M.S.)--Texas A&M University - Kingsville, 2007.
520 $a Key words. leptin, Pgf2alpha, 15-keto-Pgf2alpha, heifers
520 $a Adipocyte leptin gene expression has been reported to differ relative to stage of the heifer estrous cycle. From estrus to the mid-luteal stage of the estrous cycle, leptin gene expression remains constant. However during the late-luteal stage, leptin gene expression decreases. During this stage Pgf2alpha and its primary metabolite 15-keto-Pgf2alpha are elevated in bovine serum. Therefore, the effect of Pgf2alpha and 15-keto-Pgf2alpha on leptin and PGFr gene expression was quantified in estrogen and progesterone primed adipocytes. Subcutaneous adipose tissue (6 g) and serum was collected from the tail head region of heifers (n=10) at estrus (d 0) and the mid-luteal stage (d 10) of the estrous cycle. Adipocytes were cultured with or without Pgf2alpha or 15-keto-Pgf2alpha (10-11, 10-10, 10-9, 10-8 and 0M; n=3 wells/dose/heifer) and incubated at 37°C for 18h. Leptin and PGFr mRNA from adipose tissue and cells were quantitated using real-time PCR. Serum concentrations of Pgf2alpha and 13,14-dihydro-l5-keto-Pgf2alpha were confirmed by enzymeimmunoassay and estrogen and progesterone were confirmed by radio-immunoassay. Serum concentrations of estrogen and progesterone were greater at estrus and mid-luteal stage of the estrous cycle, respectively. Serum concentrations of Pgf2alpha and 13,14-dihydro-15-keto-Pgf2alpha did not differ relative to stage. Leptin gene expression did not differ in adipose tissue obtained at estrus or the mid-luteal. Fifteen-keto-Pgf2alpha did not affect adipocyte leptin or PGFr gene expression. Physiological concentrations of Pgf2alpha (10-8 M) tended (P=0.1) to decrease leptin gene expression in estrus adipocytes (80.5% +/- 12.7% vs. 0M, respectively). Adipocytes obtained at estrus increased (P< 0.1) PGFr gene expression after PGF2alpha treatment. Mid-luteal adipocytes did not exhibit a similar response to Pgf2alpha treatment. Hence, changes in leptin during the late-luteal stage of the estrous cycle may not be attributed to Pgf2alpha or 15-keto-Pgf2alpha alone.
590 $a School code: 1187.
650 4 $a Agriculture, Animal Culture and Nutrition. $3 1017857
650 4 $a Biology, Animal Physiology. $3 1017835
690 $a 0433
690 $a 0475
710 2 $a Texas A&M University - Kingsville. $3 1022707
773 0 $t Masters Abstracts International $g 46-01.
790 $a 1187
790 1 0 $a Garcia, Michelle R., $e advisor
791 $a M.S.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1445786