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In-vitro screening protocol for resi...

Driscoll, Jarred E.

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In-vitro screening protocol for resistance to common potato scab.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	In-vitro screening protocol for resistance to common potato scab./
Author:	Driscoll, Jarred E.
Description:	82 p.
Notes:	Adviser: David Douches.
Contained By:	Masters Abstracts International45-06.
Subject:	Agriculture, Plant Pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1444263
ISBN:	9780549019411

In-vitro screening protocol for resistance to common potato scab.
Driscoll, Jarred E.

In-vitro screening protocol for resistance to common potato scab. - 82 p.

Adviser: David Douches.

Thesis (M.S.)--Michigan State University, 2007.

Common potato scab (Streptomyces scabies) produces cork-like lesions on the outer surface of the potato (Solanum tuberosum L.) tuber, decreasing the marketability of the tubers. A protocol that is both efficient and effective in discerning resistant from susceptible varieties would be valuable. Four methods were used to assess S. scabies disease reaction on a set of potato clones (Liberator, Russet Burbank, Jacqueline Lee, and MSG227-2). The methods included, two spore suspensions with one applied to etiolated tuber sprouts and the other applied to potato tuber discs (PTDs), a thaxtomin A (TA) tuber assay, and a greenhouse assay. There was no host tissue response to either spore suspension assay and no correlation for resistance could be drawn with the TA tuber assay. Two years of field data were also collected for scab reaction on a segregating tetraploid population (MSL603, 162 individuals). Fifteen individuals from this population were selected and tested using the greenhouse assay. This assay showed a favorable response to the disease and was chosen as the method for comparing disease reaction with MSL603 population field results. Inoculated soil was used for the greenhouse study at a concentration of 3 X 10 8 CFU/cc. The greenhouse study did not correlate to the 2004 field season but did moderately correlate to 2005 (r = 0.3823, p = 0.0102) and with the averaged ratings from the 2005 and 2004 field trials (r = 0.2503, p = 0.0413). The greenhouse assay may provide data that would compliment the field data in identifying resistant clones.

ISBN: 9780549019411Subjects--Topical Terms:

1028950
Agriculture, Plant Pathology.

In-vitro screening protocol for resistance to common potato scab.
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100 1 $a Driscoll, Jarred E. $3 1272615
245 1 0 $a In-vitro screening protocol for resistance to common potato scab.
300 $a 82 p.
500 $a Adviser: David Douches.
500 $a Source: Masters Abstracts International, Volume: 45-06, page: 3014.
502 $a Thesis (M.S.)--Michigan State University, 2007.
520 $a Common potato scab (Streptomyces scabies) produces cork-like lesions on the outer surface of the potato (Solanum tuberosum L.) tuber, decreasing the marketability of the tubers. A protocol that is both efficient and effective in discerning resistant from susceptible varieties would be valuable. Four methods were used to assess S. scabies disease reaction on a set of potato clones (Liberator, Russet Burbank, Jacqueline Lee, and MSG227-2). The methods included, two spore suspensions with one applied to etiolated tuber sprouts and the other applied to potato tuber discs (PTDs), a thaxtomin A (TA) tuber assay, and a greenhouse assay. There was no host tissue response to either spore suspension assay and no correlation for resistance could be drawn with the TA tuber assay. Two years of field data were also collected for scab reaction on a segregating tetraploid population (MSL603, 162 individuals). Fifteen individuals from this population were selected and tested using the greenhouse assay. This assay showed a favorable response to the disease and was chosen as the method for comparing disease reaction with MSL603 population field results. Inoculated soil was used for the greenhouse study at a concentration of 3 X 10 8 CFU/cc. The greenhouse study did not correlate to the 2004 field season but did moderately correlate to 2005 (r = 0.3823, p = 0.0102) and with the averaged ratings from the 2005 and 2004 field trials (r = 0.2503, p = 0.0413). The greenhouse assay may provide data that would compliment the field data in identifying resistant clones.
590 $a School code: 0128.
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710 2 $a Michigan State University. $3 676168
773 0 $t Masters Abstracts International $g 45-06.
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791 $a M.S.
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856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1444263