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Occurrence and biodegradation of ant...

Cha, Jongmun.

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Occurrence and biodegradation of antibiotic compounds in the aquatic environment.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Occurrence and biodegradation of antibiotic compounds in the aquatic environment./
作者:	Cha, Jongmun.
面頁冊數:	182 p.
附註:	Adviser: Kenneth H. Carlson.
Contained By:	Dissertation Abstracts International68-08B.
標題:	Engineering, Civil. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3279497
ISBN:	9780549202387

Occurrence and biodegradation of antibiotic compounds in the aquatic environment.
Cha, Jongmun.

Occurrence and biodegradation of antibiotic compounds in the aquatic environment. - 182 p.

Adviser: Kenneth H. Carlson.

Thesis (Ph.D.)--Colorado State University, 2007.

The occurrence and fate of antibiotics in the aquatic environment are an emerging area of interest due to the potential impact of these compounds on the environment. This study applied rapid, sensitive and reliable analytical methods for the determination of beta-lactam and polyether ionophore antibiotics in surface water, wastewater, sediment, lagoon water, and animal manure. The method incorporated solid-phase extraction and liquid chromatography-ion trap tandem mass spectrometry (LC-MS-MS) with selected reaction monitoring. Specifically, the method was applied to evaluate the occurrence of these compounds in a small watershed in northern Colorado. The study also investigates the potential for on-farm removal of these compounds by measuring the biodegradation kinetics of oxytetracycline, sulfamethoxazole, tylosin, and monensin in bioreactors setup to simulate a dairy wastewater lagoon.

ISBN: 9780549202387Subjects--Topical Terms:

783781
Engineering, Civil.

Occurrence and biodegradation of antibiotic compounds in the aquatic environment.
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520 $a The occurrence and fate of antibiotics in the aquatic environment are an emerging area of interest due to the potential impact of these compounds on the environment. This study applied rapid, sensitive and reliable analytical methods for the determination of beta-lactam and polyether ionophore antibiotics in surface water, wastewater, sediment, lagoon water, and animal manure. The method incorporated solid-phase extraction and liquid chromatography-ion trap tandem mass spectrometry (LC-MS-MS) with selected reaction monitoring. Specifically, the method was applied to evaluate the occurrence of these compounds in a small watershed in northern Colorado. The study also investigates the potential for on-farm removal of these compounds by measuring the biodegradation kinetics of oxytetracycline, sulfamethoxazole, tylosin, and monensin in bioreactors setup to simulate a dairy wastewater lagoon.
520 $a Several product ions for LC-MS-MS detection of beta-lactam and ionophore compounds have been identified and documented with their proposed fragmentation pathways. This study describes statistical analysis for determination of the method detection limit, accuracy and precision of the analytical method. Consequently, the average recovery of beta-lactam and ionophore compounds from all the sample matrices was above 70% (excluding amoxicillin and ampicillin). Few of the beta-lactams under investigation could be detected due to the poor stability of the beta-lactam ring. These results indicate that the release of beta-lactams, which are commonly used for human applications, from agricultural operations may not be an environmental concern since they rapidly hydrolyze or biodegrade. The concentration of ionophores ranged from 0.03 to 0.05 mug/L for surface water samples, and from 0.5 to 3.1 mug/kg for sediment samples. Monensin (94-1077 mug/L) was also measured in runoff ponds at beef feedlots.
520 $a The research demonstrated biodegradation of oxytetracycline, sulfamethoxazole, tylosin, and monensin in dairy lagoon water at both 20°C and 4°C. However, the study found that within 175 days at 20°C and 242 days at 4°C, all antibiotics did not completely degrade under aerobic or anaerobic treatment. These results showed that degradation of antibiotics was faster under aerobic conditions, compared to anaerobic conditions and lower temperature (4°C) reduced the biodegradation rate of antibiotics.
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