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Production and antioxidant activity ...

Zhao, Bin.

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Production and antioxidant activity of raisin extracts.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Production and antioxidant activity of raisin extracts./
Author:	Zhao, Bin.
Description:	173 p.
Notes:	Adviser: Clifford Hall.
Contained By:	Dissertation Abstracts International69-03B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3305560
ISBN:	9780549519447

Production and antioxidant activity of raisin extracts.
Zhao, Bin.

Production and antioxidant activity of raisin extracts. - 173 p.

Adviser: Clifford Hall.

Thesis (Ph.D.)--North Dakota State University, 2007.

Studies were conducted to characterize the antioxidants in raisin extracts. Total phenolic content, individual phenolic compounds and hydroxymethylfurfural (HMF) content of raisin extracts were determined using spectrophotometric analysis with Folin-Ciocalteu's phenol reagent and HPLC methods. Antioxidant activity was evaluated by the Schaal oven test. Based on the antioxidant activity, the following extraction conditions were established. Ground raisins were extracted with 1:5 (g/ml) of 80% ethanol (EtOH) at pH 5 and 20°C for 25 min using sonication. After clarification of the suspension by centrifugation (3000 x g, 20°C for 25 min), the pellet was re-extracted one time.

ISBN: 9780549519447Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Production and antioxidant activity of raisin extracts.
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020 $a 9780549519447
035 $a (UMI)AAI3305560
035 $a AAI3305560
040 $a UMI $c UMI
100 1 $a Zhao, Bin. $3 1257766
245 1 0 $a Production and antioxidant activity of raisin extracts.
300 $a 173 p.
500 $a Adviser: Clifford Hall.
500 $a Source: Dissertation Abstracts International, Volume: 69-03, Section: B, page: 1390.
502 $a Thesis (Ph.D.)--North Dakota State University, 2007.
520 $a Studies were conducted to characterize the antioxidants in raisin extracts. Total phenolic content, individual phenolic compounds and hydroxymethylfurfural (HMF) content of raisin extracts were determined using spectrophotometric analysis with Folin-Ciocalteu's phenol reagent and HPLC methods. Antioxidant activity was evaluated by the Schaal oven test. Based on the antioxidant activity, the following extraction conditions were established. Ground raisins were extracted with 1:5 (g/ml) of 80% ethanol (EtOH) at pH 5 and 20°C for 25 min using sonication. After clarification of the suspension by centrifugation (3000 x g, 20°C for 25 min), the pellet was re-extracted one time.
520 $a Several methods had been used to purify phenolic compounds. The liquid-liquid extraction method concentrated the HMF, catechin, epicatechin, and ferulic acid compared to the crude raisin extracts. Solid-phase extraction, coupled with acid or alkaline hydrolysis, concentrated the epicatechin, rutin, resveratrol, and kaempferol.
520 $a The elution of the phenolic compounds using HPLC was completed using a gradient profile; solvent A was 0.07 M KH2PO4 adjusted to pH 2.5 with phosphoric acid. Solvents B and C were 100% HPLC-grade MeOH and acetonitrile, respectively. The elution program was maintained at a flow rate of 0.6 ml/min using the following gradient: solvent A (99%) and C (1%) for 25 min; solvent A (90%), B (5%), and C (5%) between 26 min and 45 min; then, a linear gradient from 90% to 30% A, from 5% to 0% C, and from 5% to 70% B in 1 min; and finally, a linear gradient from 30% to 90% A, from 0% to 1% C, and from 70% to 0% B in 19 min.
520 $a The antioxidant activities of the 3000 ppm raisin extract in bulk oils, oil-in-water emulsion, and a sunbutter system were evaluated. In the bulk oil, the 3000 ppm raisin extract had better antioxidant activity than 200 ppm tert-butylhydroquinone (TBHQ). In the o/w emulsion, the 3000 ppm raisin extract had antioxidant activity but was less than 200 ppm TBHQ. Similar to the emulsion, the 3000 ppm raisin extract had antioxidant activity in sunbutter but was less than 200 ppm TBHQ.
590 $a School code: 0157.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
690 $a 0359
710 2 $a North Dakota State University. $3 1021724
773 0 $t Dissertation Abstracts International $g 69-03B.
790 $a 0157
790 1 0 $a Hall, Clifford, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3305560