東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

From crabs to hamsters: Bioanalytica...

The University of Wisconsin - Madison.

Linked to FindBook

Google Book

Amazon

博客來

From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery./
Author:	Schmidt, Joshua John.
Description:	302 p.
Notes:	Adviser: Lingjun Li.
Contained By:	Dissertation Abstracts International68-08B.
Subject:	Biology, Bioinformatics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3279009
ISBN:	9780549190899

From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery.
Schmidt, Joshua John.

From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery. - 302 p.

Adviser: Lingjun Li.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2007.

While sometimes overlooked, peptidomics and peptide diversity is one of the most important areas of research today. This is because of the vital role that naturally created peptides play in cellular and systemic communication. Peptides have been shown to control and regulate many physiological processes, including feeding, sleep, development, and even cell death. Currently there is a lack of knowledge as to the peptide content of different but related species, as well as peptide distribution throughout nervous system within the same species. Additionally, due to integrative activity of bioactive peptides in physiological processes, there is also great potential for their use as biomarkers to indicate disease infection or progression. To begin filling in these gaps in knowledge, mass spectrometric (MS) methods have been developed for both peptidomic analysis and biomarker identification. Specifically, matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry has been used for a majority of this work due to its sensitivity, high mass measurement accuracy, and high resolution. A MALDI time-of-flight/time-of-flight instrument was also used for tissue-specific peptidomic profiling due to its sensitivity and high throughput nature. Furthermore, as the application of these methods produced a large set of data, bioinformatics methods were developed and used to process and analyze the results of MS analysis. The combination of mass spectrometry with bioinformatics has enabled rapid comparison of peptidomes of five brachyuran crabs and the determination of peptide distribution among the model nervous system and neurohemal organs of the crustacean C. borealis. Furthermore, this approach also allowed the discovery of a panel of classifying features (biomarkers) from prion infected hamsters useful in identifying infected animals. Overall, this research has developed improved methodologies and tools for peptidomic analysis and demonstrated their utility in neurochemical signaling and biomarker discovery in biological fluids.

ISBN: 9780549190899Subjects--Topical Terms:

1018415
Biology, Bioinformatics.

From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery.
LDR:03032nam 2200301 a 45 001 947683
005 20110524
008 110524s2007 ||||||||||||||||| ||eng d
020 $a 9780549190899
035 $a (UMI)AAI3279009
035 $a AAI3279009
040 $a UMI $c UMI
100 1 $a Schmidt, Joshua John. $3 1271156
245 1 0 $a From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery.
300 $a 302 p.
500 $a Adviser: Lingjun Li.
500 $a Source: Dissertation Abstracts International, Volume: 68-08, Section: B, page: 5194.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2007.
520 $a While sometimes overlooked, peptidomics and peptide diversity is one of the most important areas of research today. This is because of the vital role that naturally created peptides play in cellular and systemic communication. Peptides have been shown to control and regulate many physiological processes, including feeding, sleep, development, and even cell death. Currently there is a lack of knowledge as to the peptide content of different but related species, as well as peptide distribution throughout nervous system within the same species. Additionally, due to integrative activity of bioactive peptides in physiological processes, there is also great potential for their use as biomarkers to indicate disease infection or progression. To begin filling in these gaps in knowledge, mass spectrometric (MS) methods have been developed for both peptidomic analysis and biomarker identification. Specifically, matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry has been used for a majority of this work due to its sensitivity, high mass measurement accuracy, and high resolution. A MALDI time-of-flight/time-of-flight instrument was also used for tissue-specific peptidomic profiling due to its sensitivity and high throughput nature. Furthermore, as the application of these methods produced a large set of data, bioinformatics methods were developed and used to process and analyze the results of MS analysis. The combination of mass spectrometry with bioinformatics has enabled rapid comparison of peptidomes of five brachyuran crabs and the determination of peptide distribution among the model nervous system and neurohemal organs of the crustacean C. borealis. Furthermore, this approach also allowed the discovery of a panel of classifying features (biomarkers) from prion infected hamsters useful in identifying infected animals. Overall, this research has developed improved methodologies and tools for peptidomic analysis and demonstrated their utility in neurochemical signaling and biomarker discovery in biological fluids.
590 $a School code: 0262.
650 4 $a Biology, Bioinformatics. $3 1018415
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Health Sciences, Pharmacology. $3 1017717
650 4 $a Religion, General. $3 1017453
690 $a 0318
690 $a 0419
690 $a 0486
690 $a 0715
710 2 $a The University of Wisconsin - Madison. $3 626640
773 0 $t Dissertation Abstracts International $g 68-08B.
790 $a 0262
790 1 0 $a Li, Lingjun, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3279009