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An RNA element that stabilizes trans...

Weil, Jason E.

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An RNA element that stabilizes translation termination in Rous sarcoma virus.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	An RNA element that stabilizes translation termination in Rous sarcoma virus./
Author:	Weil, Jason E.
Description:	151 p.
Notes:	Adviser: Karen Beemon.
Contained By:	Dissertation Abstracts International68-11B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3288553
ISBN:	9780549314004

An RNA element that stabilizes translation termination in Rous sarcoma virus.
Weil, Jason E.

An RNA element that stabilizes translation termination in Rous sarcoma virus. - 151 p.

Adviser: Karen Beemon.

Thesis (Ph.D.)--The Johns Hopkins University, 2008.

The nonsense-mediated mRNA decay pathway is a cellular quality control pathway that eliminates mRNAs bearing a premature translation termination codon. Unspliced Rous sarcoma virus RNAs are subject to NMD when a premature termination codon interrupts the viral gag gene. Translation termination at the end of the gag gene does not cause this turnover due to proximity to a downstream stabilizing element that we have termed the RSV stability element (RSE). Deletion of the RSE destabilized the unspliced viral RNA. This destabilization was alleviated by arresting translation or coexpressing a dominant negative form of the protein Upf1, implicating aspects of the nonsense mediated mRNA decay pathway. Additionally, a functional fragment of the RSE can be moved after a PTC in the gag gene to prevent NMD. Interestingly, the RSE cannot stabilize termination in a spliced beta-globin RNA. A two-dimensional model describing the secondary structure of this RNA was predicted based on experimental data. The RSE is structured, and possesses several regions necessary for function. None of the other RSV genes are associated with a downstream stability element that functions like the RSE. Interestingly, a region after the gag gene of HIV-1 was able to stabilize RSV RNAs with termination codons in the gag gene, although HIV itself appears immune to NMD.

ISBN: 9780549314004Subjects--Topical Terms:

1017719
Biology, Molecular.

An RNA element that stabilizes translation termination in Rous sarcoma virus.
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020 $a 9780549314004
035 $a (UMI)AAI3288553
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040 $a UMI $c UMI
100 1 $a Weil, Jason E. $3 1269374
245 1 3 $a An RNA element that stabilizes translation termination in Rous sarcoma virus.
300 $a 151 p.
500 $a Adviser: Karen Beemon.
500 $a Source: Dissertation Abstracts International, Volume: 68-11, Section: B, page: 7132.
502 $a Thesis (Ph.D.)--The Johns Hopkins University, 2008.
520 $a The nonsense-mediated mRNA decay pathway is a cellular quality control pathway that eliminates mRNAs bearing a premature translation termination codon. Unspliced Rous sarcoma virus RNAs are subject to NMD when a premature termination codon interrupts the viral gag gene. Translation termination at the end of the gag gene does not cause this turnover due to proximity to a downstream stabilizing element that we have termed the RSV stability element (RSE). Deletion of the RSE destabilized the unspliced viral RNA. This destabilization was alleviated by arresting translation or coexpressing a dominant negative form of the protein Upf1, implicating aspects of the nonsense mediated mRNA decay pathway. Additionally, a functional fragment of the RSE can be moved after a PTC in the gag gene to prevent NMD. Interestingly, the RSE cannot stabilize termination in a spliced beta-globin RNA. A two-dimensional model describing the secondary structure of this RNA was predicted based on experimental data. The RSE is structured, and possesses several regions necessary for function. None of the other RSV genes are associated with a downstream stability element that functions like the RSE. Interestingly, a region after the gag gene of HIV-1 was able to stabilize RSV RNAs with termination codons in the gag gene, although HIV itself appears immune to NMD.
590 $a School code: 0098.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Virology. $3 1019068
690 $a 0307
690 $a 0720
710 2 $a The Johns Hopkins University. $3 1017431
773 0 $t Dissertation Abstracts International $g 68-11B.
790 $a 0098
790 1 0 $a Beemon, Karen, $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3288553