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DNA purification in microfluidic sys...

Bienvenue, Joan Marie.

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DNA purification in microfluidic systems for clinical and forensic application.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	DNA purification in microfluidic systems for clinical and forensic application./
Author:	Bienvenue, Joan Marie.
Description:	196 p.
Notes:	Adviser: James P. Landers.
Contained By:	Dissertation Abstracts International67-11B.
Subject:	Anthropology, Medical and Forensic. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3239971
ISBN:	9780542947971

DNA purification in microfluidic systems for clinical and forensic application.
Bienvenue, Joan Marie.

DNA purification in microfluidic systems for clinical and forensic application. - 196 p.

Adviser: James P. Landers.

Thesis (Ph.D.)--University of Virginia, 2007.

This dissertation describes the development of microfluidic, solid phase DNA purification methods using silica-based extraction of nucleic acids from a variety of clinically- and forensically-relevant samples. The optimization of DNA purification from semen for forensic STR amplifications is detailed, along with the evaluation of different silica solid phases. The integration of microfluidic DNA purification with microchip-based PCR amplification in a valveless system is discussed in depth, and the integrated sample preparation of a number of biological samples (blood, spleen tissue, etc.) is presented. In addition, a more complex microfluidic device is presented that fuses the on-board sample processing with electrophoretic separation/detection. This microfluidic genetic analysis system represents the first fully-integrated microfluidic device developed capable of integrated sample processing and genetic analysis. Finally, a method is presented for the electrophoretically-assisted concentration of DNA in a pressure-based purification system, with the goal of enhancing the performance of the microfluidic genetic analysis system.

ISBN: 9780542947971Subjects--Topical Terms:

1020279
Anthropology, Medical and Forensic.

DNA purification in microfluidic systems for clinical and forensic application.
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020 $a 9780542947971
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100 1 $a Bienvenue, Joan Marie. $3 1269269
245 1 0 $a DNA purification in microfluidic systems for clinical and forensic application.
300 $a 196 p.
500 $a Adviser: James P. Landers.
500 $a Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6362.
502 $a Thesis (Ph.D.)--University of Virginia, 2007.
520 $a This dissertation describes the development of microfluidic, solid phase DNA purification methods using silica-based extraction of nucleic acids from a variety of clinically- and forensically-relevant samples. The optimization of DNA purification from semen for forensic STR amplifications is detailed, along with the evaluation of different silica solid phases. The integration of microfluidic DNA purification with microchip-based PCR amplification in a valveless system is discussed in depth, and the integrated sample preparation of a number of biological samples (blood, spleen tissue, etc.) is presented. In addition, a more complex microfluidic device is presented that fuses the on-board sample processing with electrophoretic separation/detection. This microfluidic genetic analysis system represents the first fully-integrated microfluidic device developed capable of integrated sample processing and genetic analysis. Finally, a method is presented for the electrophoretically-assisted concentration of DNA in a pressure-based purification system, with the goal of enhancing the performance of the microfluidic genetic analysis system.
590 $a School code: 0246.
650 4 $a Anthropology, Medical and Forensic. $3 1020279
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0339
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710 2 $a University of Virginia. $3 645578
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790 1 0 $a Landers, James P., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3239971