東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Development of a versatile silicon-b...

Mathew, Finny P.

Linked to FindBook

Google Book

Amazon

博客來

Development of a versatile silicon-based biosensor platform for pathogen detection.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Development of a versatile silicon-based biosensor platform for pathogen detection./
Author:	Mathew, Finny P.
Description:	298 p.
Notes:	Adviser: Evangelyn C. Alocilja.
Contained By:	Dissertation Abstracts International67-10B.
Subject:	Engineering, Agricultural. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3236368
ISBN:	9780542908163

Development of a versatile silicon-based biosensor platform for pathogen detection.
Mathew, Finny P.

Development of a versatile silicon-based biosensor platform for pathogen detection. - 298 p.

Adviser: Evangelyn C. Alocilja.

Thesis (Ph.D.)--Michigan State University, 2006.

Biosensors are portable diagnostic tools used for the rapid detection of pathogens that may require up to 2-7 days to detect otherwise. Biosensors are important in the fields of human and animal diagnostics, bioterrorism preparedness, food and water safety, as well as environmental safety. So a versatile silicon-based biosensor platform for the detection of bacteria that could be used with different biological detecting elements and transducers was fabricated and demonstrated. Silicon (0.01 ohm-cm, p-type) was etched in an electrochemical cell containing hydrofluoric acid solution using anodizing conditions of 5 mA/cm2 for 1 h to fabricate nano-tubular Si (NTS). NTS chips were functionalized into biosensors using biological sensing elements such as enzymes, antibodies and nucleic acids. A chemiluminescence-based enzyme assay was adapted to the biosensor system for the detection of E. coli resulting in a lower detection limit of 102 CFU within 30 min. The NTS-based platform was also functionalized into an optical immunosensor to successfully detect a pure culture of Salmonella Typhimurium within 10 min. Finally, a DNA-based NTS biosensor was developed using an electrochemical transducer for the detection of Salmonella Enteritidis. The NTS DNA biosensor was able to detect S. Enteritidis DNA with a lower limit of detection of 1 pg/mul for PCR-amplified DNA and 10 pg/mu1 for pure culture-extracted DNA. The electrochemical detection process was completed within 60 min. The versatility of the NTS platform for pathogen detection was successfully demonstrated using three different biological sensing elements (enzyme, antibody, and DNA) and two detection methods (optical and electrochemical). Development of commercial biosensing devices using the NTS platform will strengthen biosecurity measures, enable improvement in human and animal health as well as quality assurance in food industry, while also reducing costs associated with loss of productivity, food product recalls and lawsuits.

ISBN: 9780542908163Subjects--Topical Terms:

1019504
Engineering, Agricultural.

Development of a versatile silicon-based biosensor platform for pathogen detection.
LDR:02896nam 2200265 a 45 001 945852
005 20110523
008 110523s2006 ||||||||||||||||| ||eng d
020 $a 9780542908163
035 $a (UMI)AAI3236368
035 $a AAI3236368
040 $a UMI $c UMI
100 1 $a Mathew, Finny P. $3 1269262
245 1 0 $a Development of a versatile silicon-based biosensor platform for pathogen detection.
300 $a 298 p.
500 $a Adviser: Evangelyn C. Alocilja.
500 $a Source: Dissertation Abstracts International, Volume: 67-10, Section: B, page: 5878.
502 $a Thesis (Ph.D.)--Michigan State University, 2006.
520 $a Biosensors are portable diagnostic tools used for the rapid detection of pathogens that may require up to 2-7 days to detect otherwise. Biosensors are important in the fields of human and animal diagnostics, bioterrorism preparedness, food and water safety, as well as environmental safety. So a versatile silicon-based biosensor platform for the detection of bacteria that could be used with different biological detecting elements and transducers was fabricated and demonstrated. Silicon (0.01 ohm-cm, p-type) was etched in an electrochemical cell containing hydrofluoric acid solution using anodizing conditions of 5 mA/cm2 for 1 h to fabricate nano-tubular Si (NTS). NTS chips were functionalized into biosensors using biological sensing elements such as enzymes, antibodies and nucleic acids. A chemiluminescence-based enzyme assay was adapted to the biosensor system for the detection of E. coli resulting in a lower detection limit of 102 CFU within 30 min. The NTS-based platform was also functionalized into an optical immunosensor to successfully detect a pure culture of Salmonella Typhimurium within 10 min. Finally, a DNA-based NTS biosensor was developed using an electrochemical transducer for the detection of Salmonella Enteritidis. The NTS DNA biosensor was able to detect S. Enteritidis DNA with a lower limit of detection of 1 pg/mul for PCR-amplified DNA and 10 pg/mu1 for pure culture-extracted DNA. The electrochemical detection process was completed within 60 min. The versatility of the NTS platform for pathogen detection was successfully demonstrated using three different biological sensing elements (enzyme, antibody, and DNA) and two detection methods (optical and electrochemical). Development of commercial biosensing devices using the NTS platform will strengthen biosecurity measures, enable improvement in human and animal health as well as quality assurance in food industry, while also reducing costs associated with loss of productivity, food product recalls and lawsuits.
590 $a School code: 0128.
650 4 $a Engineering, Agricultural. $3 1019504
690 $a 0539
710 2 $a Michigan State University. $3 676168
773 0 $t Dissertation Abstracts International $g 67-10B.
790 $a 0128
790 1 0 $a Alocilja, Evangelyn C., $e advisor
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3236368