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Avian immunology, immunogenetics, an...

Cheeseman, Jennifer Helen.

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Avian immunology, immunogenetics, and host immune response to Salmonella enterica serovar Enteritidis infection in chickens.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Avian immunology, immunogenetics, and host immune response to Salmonella enterica serovar Enteritidis infection in chickens./
Author:	Cheeseman, Jennifer Helen.
Description:	153 p.
Notes:	Adviser: Susan J. Lamont.
Contained By:	Dissertation Abstracts International68-12B.
Subject:	Agriculture, Animal Culture and Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3294981
ISBN:	9780549397205

Avian immunology, immunogenetics, and host immune response to Salmonella enterica serovar Enteritidis infection in chickens.
Cheeseman, Jennifer Helen.

Avian immunology, immunogenetics, and host immune response to Salmonella enterica serovar Enteritidis infection in chickens. - 153 p.

Adviser: Susan J. Lamont.

Thesis (Ph.D.)--Iowa State University, 2007.

Investigation of immune function in chickens demonstrated a role for genetics on the percentage of immune cell subsets in peripheral blood and cytokine expression. Higher cell surface expression of CD8 was observed for Egyptian Fayoumi MHC congenic lines, M15.2, and M5.1 compared to the other chicken lines examined. A two-fold lower expression of CD3 positive T cells in peripheral blood for the Leghorn MHC congenic layer lines, G-B1 and G-B2, was observed and both Leghorn lines have increased cell surface density for CD3. Examination of early gene expression in young chicks demonstrated a clear pattern in which the Leghorn line showed higher levels of splenic mRNA for IL-10, IL-12alpha, CXCLi2 (IL-8 like), and CCLI2 (MIP family member) in comparison to broilers and Fayoumis. The Leghorn line had higher IL-18, but lower CCLi2, IL-12alpha, and IL-12beta, mRNA expression in the cecum compared to broilers. Infection with Salmonella enteritidis (SE) induced the upregulation of IL-18 and IFN-gamma mRNA in the spleen and CXCLi1 and CXCLi2 (both IL-8 like) mRNA in the cecum in young chickens. Using IHC, it was noted that SE infection resulted in a decreased appearance of apoptotic cells and increased numbers of macrophages in the cecum of young birds one-week after oral inoculation. Using RNA interference (RNAi), we demonstrated siRNA-mediated knock-down of the iNOS gene in chicken macrophages. When transfected with iNOS siRNAs and later stimulated with IFN-gamma, HD-11 chicken macrophages produced significantly lower nitric oxide (NO) and had decreased iNOS mRNA transcripts compared to macrophages treated with a non-sense iNOS siRNA and IFN-gamma, or HD-11 macrophages stimulated with only IFN-gamma. Understanding the role of genetics in shaping poultry immunology, the chicken immune response to SE infection, and use of RNAi technology to investigate avian macrophage function are the purpose of this dissertation.

ISBN: 9780549397205Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

Avian immunology, immunogenetics, and host immune response to Salmonella enterica serovar Enteritidis infection in chickens.
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100 1 $a Cheeseman, Jennifer Helen. $3 1266280
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300 $a 153 p.
500 $a Adviser: Susan J. Lamont.
500 $a Source: Dissertation Abstracts International, Volume: 68-12, Section: B, page: 7720.
502 $a Thesis (Ph.D.)--Iowa State University, 2007.
520 $a Investigation of immune function in chickens demonstrated a role for genetics on the percentage of immune cell subsets in peripheral blood and cytokine expression. Higher cell surface expression of CD8 was observed for Egyptian Fayoumi MHC congenic lines, M15.2, and M5.1 compared to the other chicken lines examined. A two-fold lower expression of CD3 positive T cells in peripheral blood for the Leghorn MHC congenic layer lines, G-B1 and G-B2, was observed and both Leghorn lines have increased cell surface density for CD3. Examination of early gene expression in young chicks demonstrated a clear pattern in which the Leghorn line showed higher levels of splenic mRNA for IL-10, IL-12alpha, CXCLi2 (IL-8 like), and CCLI2 (MIP family member) in comparison to broilers and Fayoumis. The Leghorn line had higher IL-18, but lower CCLi2, IL-12alpha, and IL-12beta, mRNA expression in the cecum compared to broilers. Infection with Salmonella enteritidis (SE) induced the upregulation of IL-18 and IFN-gamma mRNA in the spleen and CXCLi1 and CXCLi2 (both IL-8 like) mRNA in the cecum in young chickens. Using IHC, it was noted that SE infection resulted in a decreased appearance of apoptotic cells and increased numbers of macrophages in the cecum of young birds one-week after oral inoculation. Using RNA interference (RNAi), we demonstrated siRNA-mediated knock-down of the iNOS gene in chicken macrophages. When transfected with iNOS siRNAs and later stimulated with IFN-gamma, HD-11 chicken macrophages produced significantly lower nitric oxide (NO) and had decreased iNOS mRNA transcripts compared to macrophages treated with a non-sense iNOS siRNA and IFN-gamma, or HD-11 macrophages stimulated with only IFN-gamma. Understanding the role of genetics in shaping poultry immunology, the chicken immune response to SE infection, and use of RNAi technology to investigate avian macrophage function are the purpose of this dissertation.
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