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Molecular genetic analysis of divisi...

Bennett, Jennifer Ann.

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Molecular genetic analysis of division and development in Streptomyces coelicolor.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Molecular genetic analysis of division and development in Streptomyces coelicolor./
Author:	Bennett, Jennifer Ann.
Description:	162 p.
Notes:	Adviser: Joseph R. McCormick.
Contained By:	Dissertation Abstracts International68-03B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3257411

Molecular genetic analysis of division and development in Streptomyces coelicolor.
Bennett, Jennifer Ann.

Molecular genetic analysis of division and development in Streptomyces coelicolor. - 162 p.

Adviser: Joseph R. McCormick.

Thesis (Ph.D.)--Duquesne University, 2007.

Here I describe both molecular and classical genetic approaches to the study of prokaryotic cell division. I detail the characterization of four cell division gene homologues: ftsL, divIC, ftsW and ftsI. FtsI, a penicillin-binding protein and transpeptidase involved specifically in septal peptidoglycan biosynthesis, is the only protein product of these four genes with a known role during cell division. I show that a null-mutation in any of the four genes resulted in a mutant with a medium-dependent division phenotype that was more severe on a rich medium. In E. coli, B. subtilis and other bacteria, these genes are essential, but in S. coelicolor they are only required for efficient division. Using phase-contrast and transmission electron microscopy, I was able to propose a role in cell division for FtsL and DivIC. In addition, I describe the development of a high efficiency, Tn5-based in vivo transposon system for the random insertional mutagenesis of Streptomyces coelicolor. This method allows high throughput screening of insertion mutants to identify novel genes involved in a variety of cellular processes. Because cell division is only required for spore formation in Streptomyces, new cell division genes can be identified by transposon mutagenesis, a strategy that would not be possible in other organisms. I have successfully conducted screens to identify transposon insertions in known developmental genes, and have also partially characterized a mutant with an interesting developmental phenotype. This mutant possessed a transposon insertion in a gene of previously unknown function, and displayed a spore shape and size defect upon examination with phasecontrast and transmission electron microscopy. Thus, I describe two different approaches to elucidate the process of cell division in S. coelicolor and other bacteria.Subjects--Topical Terms:

1017730
Biology, Genetics.

Molecular genetic analysis of division and development in Streptomyces coelicolor.
LDR:02712nam 2200265 a 45 001 940854
005 20110518
008 110518s2007 ||||||||||||||||| ||eng d
035 $a (UMI)AAI3257411
035 $a AAI3257411
040 $a UMI $c UMI
100 1 $a Bennett, Jennifer Ann. $3 1264983
245 1 0 $a Molecular genetic analysis of division and development in Streptomyces coelicolor.
300 $a 162 p.
500 $a Adviser: Joseph R. McCormick.
500 $a Source: Dissertation Abstracts International, Volume: 68-03, Section: B, page: 1420.
502 $a Thesis (Ph.D.)--Duquesne University, 2007.
520 $a Here I describe both molecular and classical genetic approaches to the study of prokaryotic cell division. I detail the characterization of four cell division gene homologues: ftsL, divIC, ftsW and ftsI. FtsI, a penicillin-binding protein and transpeptidase involved specifically in septal peptidoglycan biosynthesis, is the only protein product of these four genes with a known role during cell division. I show that a null-mutation in any of the four genes resulted in a mutant with a medium-dependent division phenotype that was more severe on a rich medium. In E. coli, B. subtilis and other bacteria, these genes are essential, but in S. coelicolor they are only required for efficient division. Using phase-contrast and transmission electron microscopy, I was able to propose a role in cell division for FtsL and DivIC. In addition, I describe the development of a high efficiency, Tn5-based in vivo transposon system for the random insertional mutagenesis of Streptomyces coelicolor. This method allows high throughput screening of insertion mutants to identify novel genes involved in a variety of cellular processes. Because cell division is only required for spore formation in Streptomyces, new cell division genes can be identified by transposon mutagenesis, a strategy that would not be possible in other organisms. I have successfully conducted screens to identify transposon insertions in known developmental genes, and have also partially characterized a mutant with an interesting developmental phenotype. This mutant possessed a transposon insertion in a gene of previously unknown function, and displayed a spore shape and size defect upon examination with phasecontrast and transmission electron microscopy. Thus, I describe two different approaches to elucidate the process of cell division in S. coelicolor and other bacteria.
590 $a School code: 0067.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0369
690 $a 0410
710 2 $a Duquesne University. $3 1017927
773 0 $t Dissertation Abstracts International $g 68-03B.
790 $a 0067
790 1 0 $a McCormick, Joseph R., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3257411