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Microbial cell preservation in a sal...

Janusz, Robert Emil.

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Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model./
Author:	Janusz, Robert Emil.
Description:	54 p.
Notes:	Adviser: Stuart J. Birnbaum.
Contained By:	Masters Abstracts International46-05.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1450636
ISBN:	9780549510284

Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model.
Janusz, Robert Emil.

Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model. - 54 p.

Adviser: Stuart J. Birnbaum.

Thesis (M.S.)--The University of Texas at San Antonio, 2008.

A laboratory scale model based on Natrialba magadii , a haloalkaliphilic archaea isolated from the saline-alkaline brine of Lake Magadi in the East African Rift Zone, was employed to investigate whether erionite, the most common zeolite mineral phase found in the trona producing areas in the lake, influences the preservation of microbial cells during evaporation. Erionite has a high cation exchange capacity and a strong affinity for K+ (Pabalan and Bertetti, 2001) and its structure contains exchangeable H2O. Haloalkaliphilic archea accumulate K + to maintain osmotic balance. Therefore, the archean cells cultured in the presence of erionite may access K+ and H2O in the erionite to maintain osmotic balance as the brine precipitates evaporite mineral phases when evaporating to dryness. Liquid media cultures of Natrialba magadii, in the presence and absence of erionite, were allowed to evaporate to dryness under aseptic conditions in a laboratory incubator. Mineral precipitates were analyzed using scanning electron microscopy, energy dispersive x-ray analysis and x-ray powder diffraction.

ISBN: 9780549510284Subjects--Topical Terms:

1017734
Biology, Microbiology.

Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model.
LDR:02666nam 2200313 a 45 001 939192
005 20110512
008 110512s2008 ||||||||||||||||| ||eng d
020 $a 9780549510284
035 $a (UMI)AAI1450636
035 $a AAI1450636
040 $a UMI $c UMI
100 1 $a Janusz, Robert Emil. $3 1263188
245 1 0 $a Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model.
300 $a 54 p.
500 $a Adviser: Stuart J. Birnbaum.
500 $a Source: Masters Abstracts International, Volume: 46-05, page: 2613.
502 $a Thesis (M.S.)--The University of Texas at San Antonio, 2008.
520 $a A laboratory scale model based on Natrialba magadii , a haloalkaliphilic archaea isolated from the saline-alkaline brine of Lake Magadi in the East African Rift Zone, was employed to investigate whether erionite, the most common zeolite mineral phase found in the trona producing areas in the lake, influences the preservation of microbial cells during evaporation. Erionite has a high cation exchange capacity and a strong affinity for K+ (Pabalan and Bertetti, 2001) and its structure contains exchangeable H2O. Haloalkaliphilic archea accumulate K + to maintain osmotic balance. Therefore, the archean cells cultured in the presence of erionite may access K+ and H2O in the erionite to maintain osmotic balance as the brine precipitates evaporite mineral phases when evaporating to dryness. Liquid media cultures of Natrialba magadii, in the presence and absence of erionite, were allowed to evaporate to dryness under aseptic conditions in a laboratory incubator. Mineral precipitates were analyzed using scanning electron microscopy, energy dispersive x-ray analysis and x-ray powder diffraction.
520 $a Upon analysis, microcosms containing both N. magadii culture and erionite revealed preserved cells. No preserved cells were seen in microcosms containing only the culture in liquid media. This observed relationship between erionite and preserved microbial "fossils" sheds light on the geomicrobiology of Natrialba magadii and zeolites and specifically the association with potassium cycling. This relationship may have significance in the study of ion exchange in zeolitic soil and water bearing zeolite rich rock substrate under saline-alkaline conditions.
590 $a School code: 1283.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Geology. $3 516570
690 $a 0372
690 $a 0410
710 2 $a The University of Texas at San Antonio. $b Earth & Environmental Science. $3 1021017
773 0 $t Masters Abstracts International $g 46-05.
790 $a 1283
790 1 0 $a Birnbaum, Stuart J., $e advisor
790 1 0 $a Bodur, Adria $e committee member
790 1 0 $a Smith, Robert K. $e committee member
791 $a M.S.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1450636