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Understanding tick salivary secretio...

Ramakrishnan, Vijay Ganesh.

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Understanding tick salivary secretions using RNA interference (RNAi).

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Understanding tick salivary secretions using RNA interference (RNAi)./
Author:	Ramakrishnan, Vijay Ganesh.
Description:	151 p.
Notes:	Adviser: Richard C. Essenberg.
Contained By:	Dissertation Abstracts International67-12B.
Subject:	Biology, Entomology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3245762

Understanding tick salivary secretions using RNA interference (RNAi).
Ramakrishnan, Vijay Ganesh.

Understanding tick salivary secretions using RNA interference (RNAi). - 151 p.

Adviser: Richard C. Essenberg.

Thesis (D.Phil.)--Oklahoma State University, 2007.

RNAi is a recently discovered powerful tool to silence gene expression in a sequence specific manner at the mRNA level. Protein secretion from the female tick salivary glands occur by regulated exocytosis and requires the participation of proteins called SNAREs (Soluble N-ethylmaleimide sensitive factor Attachment protein Receptor). Members of this class of proteins are expressed in all eukaryotic cells and are designated as vesicle (v-SNARE) and target (t-SNARE) SNARE depending on the membranes they are associated with. Regulated exocytosis, in addition, requires the functions of several SNARE interacting proteins. Synaptobrevin is a key v-SNARE and nSec1 is a key t-SNARE interacting protein in many secreting cells studied till date. cDNA fragment homologues of synaptobrevin and nSec1 were cloned and sequenced from the salivary glands of partially fed female ticks. Synaptobrevin and nSec1 double stranded RNAs (dsRNA) were used to specifically reduce the amount of synaptobrevin and nSec1 mRNA and protein levels respectively. This decrease was accompanied by a decrease in anticoagulant protein release from the glands. In addition, nSec1 dsRNA was injected into unfed female ticks and allowed to feed on sheep. We were able to demonstrate differences in the transcript and protein levels with reduced protein secretion and slower growth patterns in ticks injected with dsRNA.Subjects--Topical Terms:

1018619
Biology, Entomology.

Understanding tick salivary secretions using RNA interference (RNAi).
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005 20110512
008 110512s2007 eng d
035 $a (UMI)AAI3245762
035 $a AAI3245762
040 $a UMI $c UMI
100 1 $a Ramakrishnan, Vijay Ganesh. $3 1262871
245 1 0 $a Understanding tick salivary secretions using RNA interference (RNAi).
300 $a 151 p.
500 $a Adviser: Richard C. Essenberg.
500 $a Source: Dissertation Abstracts International, Volume: 67-12, Section: B, page: 6901.
502 $a Thesis (D.Phil.)--Oklahoma State University, 2007.
520 $a RNAi is a recently discovered powerful tool to silence gene expression in a sequence specific manner at the mRNA level. Protein secretion from the female tick salivary glands occur by regulated exocytosis and requires the participation of proteins called SNAREs (Soluble N-ethylmaleimide sensitive factor Attachment protein Receptor). Members of this class of proteins are expressed in all eukaryotic cells and are designated as vesicle (v-SNARE) and target (t-SNARE) SNARE depending on the membranes they are associated with. Regulated exocytosis, in addition, requires the functions of several SNARE interacting proteins. Synaptobrevin is a key v-SNARE and nSec1 is a key t-SNARE interacting protein in many secreting cells studied till date. cDNA fragment homologues of synaptobrevin and nSec1 were cloned and sequenced from the salivary glands of partially fed female ticks. Synaptobrevin and nSec1 double stranded RNAs (dsRNA) were used to specifically reduce the amount of synaptobrevin and nSec1 mRNA and protein levels respectively. This decrease was accompanied by a decrease in anticoagulant protein release from the glands. In addition, nSec1 dsRNA was injected into unfed female ticks and allowed to feed on sheep. We were able to demonstrate differences in the transcript and protein levels with reduced protein secretion and slower growth patterns in ticks injected with dsRNA.
590 $a School code: 0664.
650 4 $a Biology, Entomology. $3 1018619
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Parasitology. $3 1028974
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0307
690 $a 0353
690 $a 0487
690 $a 0718
710 2 0 $a Oklahoma State University. $3 626630
773 0 $t Dissertation Abstracts International $g 67-12B.
790 $a 0664
790 1 0 $a Essenberg, Richard C., $e advisor
791 $a D.Phil.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3245762