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Functional estrogen receptors in art...

Richmond, Renee Smith.

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Functional estrogen receptors in articular cartilage: Estrogen effects on chondrocytes and characterization of estrogen receptors in human monocyte-derived macrophages.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Functional estrogen receptors in articular cartilage: Estrogen effects on chondrocytes and characterization of estrogen receptors in human monocyte-derived macrophages./
Author:	Richmond, Renee Smith.
Description:	227 p.
Notes:	Adviser: Iris J. Edwards.
Contained By:	Dissertation Abstracts International63-02B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3043103
ISBN:	0493566775

Functional estrogen receptors in articular cartilage: Estrogen effects on chondrocytes and characterization of estrogen receptors in human monocyte-derived macrophages.
Richmond, Renee Smith.

Functional estrogen receptors in articular cartilage: Estrogen effects on chondrocytes and characterization of estrogen receptors in human monocyte-derived macrophages. - 227 p.

Adviser: Iris J. Edwards.

Thesis (Ph.D.)--Wake Forest University, The Bowman Gray School of Medicine, 2002.

Epidemiological studies suggest that estrogen replacement therapy (ERT) is protective against the development of both osteoarthritis and atherosclerosis. The focus of this work is the interaction of estrogen with cells intimately associated with each of these pathological processes. Studies were conducted to determine if functional estrogen receptors (ER) are present in adult articular cartilage and to determine if ERT effects <italic> in vivo</italic> may be mediated by insulin-like growth factor binding proteins (IGFBPs). mRNA for ERα and ERβ was present in adult monkey articular cartilage and ER protein was demonstrated by immunoblotting and immunohistochemistry. Estrogen treatment of chondrocytes transfected with an ERE/Luciferase construct resulted in a significant increase in reporter production (2.87-fold above control, p < 0.02) indicating a functional ER. In primary chondrocytes cultured from monkeys receiving ERT versus no treatment, IGFBP-2 production was increased significantly (p < 0.008). ERT was associated with a significant increase in proteoglycan synthesis in these cells (1.41-fold above control, p < 0.02). These results indicate that estrogen has direct effects on adult articular cartilage and may be a key to how ERT is beneficial to joint metabolism.

ISBN: 0493566775Subjects--Topical Terms:

1017686
Biology, Cell.

Functional estrogen receptors in articular cartilage: Estrogen effects on chondrocytes and characterization of estrogen receptors in human monocyte-derived macrophages.
LDR:03406nam 2200313 a 45 001 938783
005 20110512
008 110512s2002 eng d
020 $a 0493566775
035 $a (UnM)AAI3043103
035 $a AAI3043103
040 $a UnM $c UnM
100 1 $a Richmond, Renee Smith. $3 1262756
245 1 0 $a Functional estrogen receptors in articular cartilage: Estrogen effects on chondrocytes and characterization of estrogen receptors in human monocyte-derived macrophages.
300 $a 227 p.
500 $a Adviser: Iris J. Edwards.
500 $a Source: Dissertation Abstracts International, Volume: 63-02, Section: B, page: 0671.
502 $a Thesis (Ph.D.)--Wake Forest University, The Bowman Gray School of Medicine, 2002.
520 $a Epidemiological studies suggest that estrogen replacement therapy (ERT) is protective against the development of both osteoarthritis and atherosclerosis. The focus of this work is the interaction of estrogen with cells intimately associated with each of these pathological processes. Studies were conducted to determine if functional estrogen receptors (ER) are present in adult articular cartilage and to determine if ERT effects <italic> in vivo</italic> may be mediated by insulin-like growth factor binding proteins (IGFBPs). mRNA for ERα and ERβ was present in adult monkey articular cartilage and ER protein was demonstrated by immunoblotting and immunohistochemistry. Estrogen treatment of chondrocytes transfected with an ERE/Luciferase construct resulted in a significant increase in reporter production (2.87-fold above control, p < 0.02) indicating a functional ER. In primary chondrocytes cultured from monkeys receiving ERT versus no treatment, IGFBP-2 production was increased significantly (p < 0.008). ERT was associated with a significant increase in proteoglycan synthesis in these cells (1.41-fold above control, p < 0.02). These results indicate that estrogen has direct effects on adult articular cartilage and may be a key to how ERT is beneficial to joint metabolism.
520 $a Studies directed towards a mechanism for estrogen protection in atherosclerosis focused on human monocyte derived macrophages (HMDM), cells that accumulate in atherosclerotic lesions. Cells were isolated from peripheral blood of male and female donors, separated by Isolymph gradient and differential adherence, then cultured up to 14 days. ERα RT-PCR product was undetectable in initial cultures (day 0), but was expressed from day 5 through 14. ERβ was detected only at day 0, and data suggested this was due to contaminating lymphocytes. These expression patterns were neither gender specific nor regulated by estrogen. ERα expression was approximately 20% that of ovary, but greater than cultured arterial smooth muscle cells. Day 7 HMDM had distinct nuclear ERα staining and bound estrogen, but to a lesser extent than MCF-7 cells. These studies demonstrate that HMDM are a potential target for estrogen and as such may be involved in estrogen-mediated protection against early events in atherosclerosis.
590 $a School code: 0249.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Health Sciences, Medicine and Surgery. $3 1017756
650 4 $a Health Sciences, Pharmacology. $3 1017717
690 $a 0307
690 $a 0379
690 $a 0419
690 $a 0564
710 2 0 $a Wake Forest University, The Bowman Gray School of Medicine. $3 1251076
773 0 $t Dissertation Abstracts International $g 63-02B.
790 $a 0249
790 1 0 $a Edwards, Iris J., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3043103