東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Characterization of voltage-gated so...

Linares, Alicia Esther.

FindBook

Google Book

Amazon

博客來

Characterization of voltage-gated sodium channel genes expressed in zebrafish embryos.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Characterization of voltage-gated sodium channel genes expressed in zebrafish embryos./
作者:	Linares, Alicia Esther.
面頁冊數:	242 p.
附註:	Adviser: Angeles Ribera.
Contained By:	Dissertation Abstracts International63-11B.
標題:	Biology, Animal Physiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3069588
ISBN:	0493892966

Characterization of voltage-gated sodium channel genes expressed in zebrafish embryos.
Linares, Alicia Esther.

Characterization of voltage-gated sodium channel genes expressed in zebrafish embryos. - 242 p.

Adviser: Angeles Ribera.

Thesis (Ph.D.)--University of Colorado Health Sciences Center, 2002.

Assignment of specific functions to the large number of ion channels is a difficult task. Some light has been shed on this problem with the discovery of human ion channelopathies. However, the symptoms of most channelopathies are present only in the adult. Thus, these conditions offer little insight into the roles ion channels might play during embryonic development. Large-scale mutagenesis screens in zebrafish have provided phenotypic candidates for mutated ion channel genes in the embryo. One of these mutants, macho (<italic>mao </italic>), has an altered touch response (at 27 hpf) and reduction in sodium current amplitude in mechanosensory spinal cord neurons (R-B cells) and retinal ganglion cells. These data suggest that <italic>mao</italic> is a gene required for formation of functional sodium channels. Thus, I hypothesized that <italic> mao</italic> codes for a structural alpha or NaV1 gene expressed in R-B cells during embryonic development.

ISBN: 0493892966Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Characterization of voltage-gated sodium channel genes expressed in zebrafish embryos.
LDR:03528nam 2200325 a 45 001 934730
005 20110509
008 110509s2002 eng d
020 $a 0493892966
035 $a (UnM)AAI3069588
035 $a AAI3069588
040 $a UnM $c UnM
100 1 $a Linares, Alicia Esther. $3 1258429
245 1 0 $a Characterization of voltage-gated sodium channel genes expressed in zebrafish embryos.
300 $a 242 p.
500 $a Adviser: Angeles Ribera.
500 $a Source: Dissertation Abstracts International, Volume: 63-11, Section: B, page: 5087.
502 $a Thesis (Ph.D.)--University of Colorado Health Sciences Center, 2002.
520 $a Assignment of specific functions to the large number of ion channels is a difficult task. Some light has been shed on this problem with the discovery of human ion channelopathies. However, the symptoms of most channelopathies are present only in the adult. Thus, these conditions offer little insight into the roles ion channels might play during embryonic development. Large-scale mutagenesis screens in zebrafish have provided phenotypic candidates for mutated ion channel genes in the embryo. One of these mutants, macho (<italic>mao </italic>), has an altered touch response (at 27 hpf) and reduction in sodium current amplitude in mechanosensory spinal cord neurons (R-B cells) and retinal ganglion cells. These data suggest that <italic>mao</italic> is a gene required for formation of functional sodium channels. Thus, I hypothesized that <italic> mao</italic> codes for a structural alpha or NaV1 gene expressed in R-B cells during embryonic development.
520 $a Five zebrafish NaV1 cDNA clones were isolated in this study to provide candidates for <italic>mao</italic>. Based on amino acid identity with mammalian NaV1 subunits and <italic>in situ</italic> hybridization patterns, orthologues of NaV1.2, NaV1.4, NaV1.5, NaV1.6 and NaV1.7 were assigned to the isolated zebrafish cDNAs. Further, NaV1.6 and NaV1.7 were the best candidates for <italic>mao</italic> based on their mRNA expression patterns in the R-B cells.
520 $a Using gene mapping, the genomic locations of zNaV1.6 and zNa V1.7 were compared to that of the <italic>mao</italic> mutation. These data indicate that neither zNaV1.6 nor zNaV1.7 is the gene that harbors the <italic>mao</italic> mutation. In addition, the mapping and molecular data gleaned from the zNaV1 genes provides a unique look at how NaV1 genes may have evolved in the zebrafish. Two new hypotheses were also tested about where the <italic>mao</italic> mutation may lie in the formation of a sodium channel complex containing zNaV 1.6 and/or zNaV1.7 channels in R-B cells.
520 $a In sum, many good candidates for <italic>mao</italic> have been ruled out in these studies. With the completion of the zebrafish genome on the horizon (2003), the role of many new genes that function in the sodium channel pathway will be elucidated, revealing new genes involved in ion channel function as well as the identity of <italic>mao</italic>.
590 $a School code: 0831.
650 4 $a Biology, Animal Physiology. $3 1017835
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Neuroscience. $3 1017680
690 $a 0307
690 $a 0317
690 $a 0433
710 2 0 $a University of Colorado Health Sciences Center. $3 1023815
773 0 $t Dissertation Abstracts International $g 63-11B.
790 $a 0831
790 1 0 $a Ribera, Angeles, $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3069588