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Studies of carcinogen-modified DNA a...

Li, Linge.

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Studies of carcinogen-modified DNA adducts by high performance liquid chromatography and mass spectrometry.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Studies of carcinogen-modified DNA adducts by high performance liquid chromatography and mass spectrometry./
Author:	Li, Linge.
Description:	148 p.
Notes:	Adviser: M. Paul Chiarelli.
Contained By:	Dissertation Abstracts International64-03B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3085090

Studies of carcinogen-modified DNA adducts by high performance liquid chromatography and mass spectrometry.
Li, Linge.

Studies of carcinogen-modified DNA adducts by high performance liquid chromatography and mass spectrometry. - 148 p.

Adviser: M. Paul Chiarelli.

Thesis (Ph.D.)--Loyola University of Chicago, 2003.

This dissertation describes different analytical approaches for the detection and structural differentiation of aromatic carcinogen-modified DNA using mass spectrometry. Separation, detection and quantitation of aromatic-modified DNA adducts were carried out using micro-HPLC/UV spectrometry and nano-LC/ESI ion trap mass spectrometry. An on-line method of solid-phase extraction (SPE) followed by HPLC was developed to isolate ultra trace amount of DNA adducts from large quantities of normal DNA. This method was accomplished by coupling micro-HPLC with a valve-switching technique. The detection of aromatic-modified DNA adducts at &sim;1 fmol (10<super>−15</super> mole) quantity using SIM and SRM detection modes was demonstrated using an ion trap mass spectrometry coupled with nano-electrospray ionization. In addition, a linear correlation between the signal intensity and the amount of analyte injected was established in the range of 1.5 fmol to 168 fmol quantity, suggesting quantification for aromatic-modified DNA adducts may be achieved on nano-LC/ESI/MS. Moreover, the analysis of a DNA adduct mixture, even in the presence of large excess of normal deoxynucleosides (in a DNA digest), was successfully demonstrated on the nano-LC/ESI ion trap mass spectrometry.Subjects--Topical Terms:

586156
Chemistry, Analytical.

Studies of carcinogen-modified DNA adducts by high performance liquid chromatography and mass spectrometry.
LDR:03143nam 2200265 a 45 001 934706
005 20110509
008 110509s2003 eng d
035 $a (UnM)AAI3085090
035 $a AAI3085090
040 $a UnM $c UnM
100 1 $a Li, Linge. $3 1258405
245 1 0 $a Studies of carcinogen-modified DNA adducts by high performance liquid chromatography and mass spectrometry.
300 $a 148 p.
500 $a Adviser: M. Paul Chiarelli.
500 $a Source: Dissertation Abstracts International, Volume: 64-03, Section: B, page: 1224.
502 $a Thesis (Ph.D.)--Loyola University of Chicago, 2003.
520 $a This dissertation describes different analytical approaches for the detection and structural differentiation of aromatic carcinogen-modified DNA using mass spectrometry. Separation, detection and quantitation of aromatic-modified DNA adducts were carried out using micro-HPLC/UV spectrometry and nano-LC/ESI ion trap mass spectrometry. An on-line method of solid-phase extraction (SPE) followed by HPLC was developed to isolate ultra trace amount of DNA adducts from large quantities of normal DNA. This method was accomplished by coupling micro-HPLC with a valve-switching technique. The detection of aromatic-modified DNA adducts at &sim;1 fmol (10<super>−15</super> mole) quantity using SIM and SRM detection modes was demonstrated using an ion trap mass spectrometry coupled with nano-electrospray ionization. In addition, a linear correlation between the signal intensity and the amount of analyte injected was established in the range of 1.5 fmol to 168 fmol quantity, suggesting quantification for aromatic-modified DNA adducts may be achieved on nano-LC/ESI/MS. Moreover, the analysis of a DNA adduct mixture, even in the presence of large excess of normal deoxynucleosides (in a DNA digest), was successfully demonstrated on the nano-LC/ESI ion trap mass spectrometry.
520 $a The structural characterization and differentiation of carcinogen-modified DNA adducts was accomplished by tandem mass spectrometry (MS/MS). The product ion formation characteristics of thirteen different C8-substituted alkylaniline adducts of guanine were studied using ESI quadrupole ion trap tandem mass spectrometry. A similarity index (SI) was used to differentiate the structures of isomers. The structures of all isomeric BH<sub>2</sub><super>+</super> ions studied here could be differentiated based on the calculation of the similarity index using five or six product ions. The product ion spectra acquired over a two month period with the ion trap were found to be sufficiently reproducible to give almost identical Sls for these DNA adducts as well. These results suggest that a searchable database of product ion spectra may be created and used to identify unknown guanine adducts when they are detected with structural information. (Abstract shortened by UMI.)
590 $a School code: 0112.
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0486
710 2 0 $a Loyola University of Chicago. $3 1249345
773 0 $t Dissertation Abstracts International $g 64-03B.
790 $a 0112
790 1 0 $a Chiarelli, M. Paul, $e advisor
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3085090