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Forward and reverse chemical genetic...

Stockwell, Brent Roark.

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Forward and reverse chemical genetic studies of transforming growth factor beta signaling.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Forward and reverse chemical genetic studies of transforming growth factor beta signaling./
Author:	Stockwell, Brent Roark.
Description:	137 p.
Notes:	Adviser: Stuart L. Schreiber.
Contained By:	Dissertation Abstracts International60-11B.
Subject:	Chemistry, Biochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9949810
ISBN:	0599522046

Forward and reverse chemical genetic studies of transforming growth factor beta signaling.
Stockwell, Brent Roark.

Forward and reverse chemical genetic studies of transforming growth factor beta signaling. - 137 p.

Adviser: Stuart L. Schreiber.

Thesis (Ph.D.)--Harvard University, 1999.

A forward genetic approach involves phenotype-based screens for genes affecting a process of interest, whereas a reverse genetic approach involves alteration of a particular gene and description of the resulting phenotype. These approaches, when extended to functional perturbations caused by small molecules instead of mutations, are termed forward and reverse chemical genetics. We applied these methods of analysis to transforming growth factor beta (TGF-β) signaling.

ISBN: 0599522046Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Forward and reverse chemical genetic studies of transforming growth factor beta signaling.
LDR:02280nam 2200313 a 45 001 933564
005 20110505
008 110505s1999 eng d
020 $a 0599522046
035 $a (UnM)AAI9949810
035 $a AAI9949810
040 $a UnM $c UnM
100 1 $a Stockwell, Brent Roark. $3 1257293
245 1 0 $a Forward and reverse chemical genetic studies of transforming growth factor beta signaling.
300 $a 137 p.
500 $a Adviser: Stuart L. Schreiber.
500 $a Source: Dissertation Abstracts International, Volume: 60-11, Section: B, page: 5504.
502 $a Thesis (Ph.D.)--Harvard University, 1999.
520 $a A forward genetic approach involves phenotype-based screens for genes affecting a process of interest, whereas a reverse genetic approach involves alteration of a particular gene and description of the resulting phenotype. These approaches, when extended to functional perturbations caused by small molecules instead of mutations, are termed forward and reverse chemical genetics. We applied these methods of analysis to transforming growth factor beta (TGF-β) signaling.
520 $a We used a reverse chemical genetic approach to study the TGF-β receptors. Thus, small molecules were used to activate the TGF-β receptors conditionally. The role of each receptor in inducing the phenotypic changes caused by TGF-β was assessed. Such phenotypic changes included Smad2 nuclear translocation, reporter gene activation, and growth inhibition of mink lung cells.
520 $a We also used a forward chemical genetic approach to study TGF-β signaling. We developed high throughput assays for small molecule modifiers of TGF-β signaling, including a generally useful antibody-based assay referred to as a cytoblot. We identified small molecule activators of a TGF-β-responsive reporter gene, and demonstrated the effectiveness of whole-genome transcriptional profiling to study the mechanisms by which these small molecules act.
590 $a School code: 0084.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Organic. $3 516206
650 4 $a Chemistry, Pharmaceutical. $3 550957
690 $a 0487
690 $a 0490
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710 2 0 $a Harvard University. $3 528741
773 0 $t Dissertation Abstracts International $g 60-11B.
790 $a 0084
790 1 0 $a Schreiber, Stuart L., $e advisor
791 $a Ph.D.
792 $a 1999
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9949810