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Polyhydroxyalkanoate synthesis in re...

Aldor, Ilana Sharon.

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Polyhydroxyalkanoate synthesis in recombinant and environmental microbes.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Polyhydroxyalkanoate synthesis in recombinant and environmental microbes./
Author:	Aldor, Ilana Sharon.
Description:	114 p.
Notes:	Chair: Jay D. Keasling.
Contained By:	Dissertation Abstracts International63-02B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3044366
ISBN:	0493582118

Polyhydroxyalkanoate synthesis in recombinant and environmental microbes.
Aldor, Ilana Sharon.

Polyhydroxyalkanoate synthesis in recombinant and environmental microbes. - 114 p.

Chair: Jay D. Keasling.

Thesis (Ph.D.)--University of California, Berkeley, 2001.

The main focus has been on the copolymer poly(3-hydroxybutyrate-<italic> co</italic>-3-hydroxyvalerate) (PHBV). It has commercially desirable physical and mechanical properties that are determined by its 3-hydroxyvalerate (HV) content.

ISBN: 0493582118Subjects--Topical Terms:

1017734
Biology, Microbiology.

Polyhydroxyalkanoate synthesis in recombinant and environmental microbes.
LDR:03663nam 2200361 a 45 001 933139
005 20110505
008 110505s2001 eng d
020 $a 0493582118
035 $a (UnM)AAI3044366
035 $a AAI3044366
040 $a UnM $c UnM
100 1 $a Aldor, Ilana Sharon. $3 1256879
245 1 0 $a Polyhydroxyalkanoate synthesis in recombinant and environmental microbes.
300 $a 114 p.
500 $a Chair: Jay D. Keasling.
500 $a Source: Dissertation Abstracts International, Volume: 63-02, Section: B, page: 0900.
502 $a Thesis (Ph.D.)--University of California, Berkeley, 2001.
520 $a The main focus has been on the copolymer poly(3-hydroxybutyrate-<italic> co</italic>-3-hydroxyvalerate) (PHBV). It has commercially desirable physical and mechanical properties that are determined by its 3-hydroxyvalerate (HV) content.
520 $a Polyhydroxyalkanoates (PHAs) represent a class of natural polyesters that are biodegradable and made from renewable resources. These biopolymers offer a sustainable alternative to petrochemical-derived plastics. New expression systems and metabolic engineering strategies have been developed, and novel biosynthetic genes have been discovered to facilitate the use of recombinant microbes for PHA bioplastics production.
520 $a PHBV synthesis was reconstituted in recombinant <italic>E. coli</italic>, and the effects of varying factors such as minimal media, carbon source, vector backbone, and PHA synthesis operon source were surveyed. Results indicated that all these variables were important in determining copolymer content and composition. Furthermore, the discovery that expression of the PHA synthesis operon from <italic>Acinetobacter</italic> sp. RA3849 led to a higher HV fraction in the copolymer than the expression of the <italic>Ralstonia eutropha</italic> operon provided a tool for the metabolic engineering of PHBV composition.
520 $a To control copolymer composition, a strain of <italic>Salmonella enterica </italic> serovar Typhimurium mutant in the ability to convert propionate to propionyl-CoA (the key precursor in the synthesis of HV) was metabolically engineered with the pathways for propionyl-CoA and polymer synthesis. A system has been developed in which the inducible expression of the propionyl-CoA synthetase gene (<italic>prpE</italic>) is varied while the <italic>Acinetobacter </italic> PHA synthesis operon (<italic>phaBCA</italic>) is coexpressed under the control of an independent promoter at a fixed substrate (glycerol and propionate) concentration. Using this expression system, copolymer composition can be controlled between 2 and 25 mol % HV by varying the IPTG level in the medium. This may have application in making biodegradable plastics with specific properties for processes where the propionate composition of the feed steam is not adjustable.
520 $a Finally, PCR-based molecular techniques were used to identify unique PHA synthase genes in the environmental microorganisms in a lab-scale activated sludge reactor performing enhanced biological phosphorus removal. Nine novel PHA synthase fragments were discovered. This may eventually lead to the metabolic engineering of new PHA copolymers with interesting properties for sustainable plastics production in recombinant microbes.
590 $a School code: 0028.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Engineering, Chemical. $3 1018531
650 4 $a Engineering, Environmental. $3 783782
650 4 $a Plastics Technology. $3 1023683
690 $a 0307
690 $a 0410
690 $a 0542
690 $a 0775
690 $a 0795
710 2 0 $a University of California, Berkeley. $3 687832
773 0 $t Dissertation Abstracts International $g 63-02B.
790 $a 0028
790 1 0 $a Keasling, Jay D., $e advisor
791 $a Ph.D.
792 $a 2001
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3044366