東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Effects of fumonisin B1 on avian imm...

Boone, Becky J.

Linked to FindBook

Google Book

Amazon

博客來

Effects of fumonisin B1 on avian immune responses.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Effects of fumonisin B1 on avian immune responses./
Author:	Boone, Becky J.
Description:	97 p.
Notes:	Adviser: A. B. Bodine.
Contained By:	Dissertation Abstracts International62-10B.
Subject:	Biology, Animal Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3031108
ISBN:	0493433457

Effects of fumonisin B1 on avian immune responses.
Boone, Becky J.

Effects of fumonisin B1 on avian immune responses. - 97 p.

Adviser: A. B. Bodine.

Thesis (Ph.D.)--Clemson University, 2001.

The effects of fumonisin B1 (FB1) on the avian immune cell responses were investigated. Lymphocytes were collected from spleen, thymus and peripheral blood (PBL). Macrophages were collected from spleen and from the peritoneal cavity. The effects of varying concentrations of FB 1 (5 to 50 μg/ml) on lymphocyte viability, mitogenic response and macrophage activation were assessed using the MTT reduction assay. In addition, macrophage morphology, phagocytic ability and nitric oxide (NO) production were assessed in cells exposed to 5 to 50 μg/ml FB1. Finally, the effects of FB1 on induction of apoptosis and caspase-3 activity in splenocytes and thymocytes were investigated.

ISBN: 0493433457Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Effects of fumonisin B1 on avian immune responses.
LDR:03356nam 2200313 a 45 001 932983
005 20110505
008 110505s2001 eng d
020 $a 0493433457
035 $a (UnM)AAI3031108
035 $a AAI3031108
040 $a UnM $c UnM
100 1 $a Boone, Becky J. $3 1256723
245 1 0 $a Effects of fumonisin B1 on avian immune responses.
300 $a 97 p.
500 $a Adviser: A. B. Bodine.
500 $a Source: Dissertation Abstracts International, Volume: 62-10, Section: B, page: 4301.
502 $a Thesis (Ph.D.)--Clemson University, 2001.
520 $a The effects of fumonisin B1 (FB1) on the avian immune cell responses were investigated. Lymphocytes were collected from spleen, thymus and peripheral blood (PBL). Macrophages were collected from spleen and from the peritoneal cavity. The effects of varying concentrations of FB 1 (5 to 50 μg/ml) on lymphocyte viability, mitogenic response and macrophage activation were assessed using the MTT reduction assay. In addition, macrophage morphology, phagocytic ability and nitric oxide (NO) production were assessed in cells exposed to 5 to 50 μg/ml FB1. Finally, the effects of FB1 on induction of apoptosis and caspase-3 activity in splenocytes and thymocytes were investigated.
520 $a Fumonisin B1 decreased spleen cell viability, but had no effect on PBL viability. In thymocytes, an increase in viability was seen, however a statistical difference (P ≤ .05) was achieved only at the 50 μg/ml FB1. The effects of FB1 on the mitogenic response were evaluated in splenocytes and PBLs stimulated with the mitogens, lipopolysacchride (LPS), Concanavalin A (Con A) and Pokeweed mitogen (PWM) and thymocytes stimulated with Con A. Mitogenic response declined for all mitogens in FBI treated splenocytes; albeit, the degree of decrease varied with mitogen and time of exposure. No effect on mitogenic response was observed in FBI treated cells from the thymus or PBL.
520 $a Fumonisin B1 decreased cell activity in macrophages recruited from the peritoneum, yet increased the splenic macrophage activity. Nitric oxide production increased in all macrophage populations investigation, such that at 50 μg/ml FB1 NO production was increased approximately 2-fold in all cells. Finally, FB1 caused an increase in vacuolization in macrophages and decreased the ability of the cells to uptake sheep red blood cells.
520 $a The effects of FB1 on apoptosis processes were investigated as well. Agarose gel electrophoresis showed FB1 induced apoptosis in splenocytes. Fumonisin B1 did not effect caspase-3 activity within these cells, whereas, caspase-3 activity increased in thymocytes exposed to FB1. Through the use of exogenous sphingosine and the serine palmitoyltransferase inhibitor, myriocin, it was shown that the induction of caspase is probably associated to the increase in sphingosine induced by FB1.
590 $a School code: 0050.
650 4 $a Biology, Animal Physiology. $3 1017835
650 4 $a Health Sciences, Toxicology. $3 1017752
690 $a 0383
690 $a 0433
710 2 0 $a Clemson University. $3 997173
773 0 $t Dissertation Abstracts International $g 62-10B.
790 $a 0050
790 1 0 $a Bodine, A. B., $e advisor
791 $a Ph.D.
792 $a 2001
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3031108