東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

The role of zinc in DNA damage and r...

Trapp, Christine Lee.

FindBook

Google Book

Amazon

博客來

The role of zinc in DNA damage and repair.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	The role of zinc in DNA damage and repair./
作者:	Trapp, Christine Lee.
面頁冊數:	129 p.
附註:	Source: Dissertation Abstracts International, Volume: 63-05, Section: B, page: 2318.
Contained By:	Dissertation Abstracts International63-05B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3051557
ISBN:	0493663045

The role of zinc in DNA damage and repair.
Trapp, Christine Lee.

The role of zinc in DNA damage and repair. - 129 p.

Source: Dissertation Abstracts International, Volume: 63-05, Section: B, page: 2318.

Thesis (Ph.D.)--University of California, Davis, 2002.

Zn is essential for the proper functioning of over 200 enzymes and plays a major role in genetic expression, cell division and growth. Poly (ADP-ribose) polymerase (PARP) is a zinc-containing enzyme that is important in cell differentiation, gene expression and DNA replication and repair. Dialysis of PARP, under a variety of conditions, indicates that a decrease in zinc content of the enzyme is associated with a decrease in activity. I hypothesized that a nutritional zinc deficiency would result in decreased PARP activity. 3T3 fibroblasts were grown in media with varying levels of zinc (0.5, 6, 15, 45 and 90 μM). The cells were grown in experimental media for 48 h and harvested during log phase growth. PARP activity was determined by measuring the incorporation of [32P] NAD into acid-insoluble acceptors. PARP protein was assessed by western blot analysis. Ku80 expression, a non-zinc DNA repair enzyme was measured as a control. PARP activity correlated positively with media zinc concentration. Cells grown in zinc deficient media (0.5μM zinc) had PARP activity 1/10th the activity of cells grown in media containing 90μM zinc. Cells grown in 90μM zinc had a similar PARP activity as cells grown in 10% FBS. Western analysis indicated decreased levels of PARP protein at the lowest levels of zinc (0.5 and 6 μM), whereas, Ku80 expression increased. A decrease in the activity of PARP could, in part, be responsible for abnormalities of gene expression, cell division and growth seen during zinc deficiency. Day 11 rat embryos, from dams fed zinc-deficient diets however, did not have significantly lower PARP activity, despite typical teratologies. These results show, that while zinc-deficiency can have a marked effect on PARP activity in isolated rapidly growing cells, this effect is not observed on a global basis in the whole embryo. The above results suggest that while reductions in DNA repair may contribute to the teratology associated with zinc deficiency it is not a major mechanism.

ISBN: 0493663045Subjects--Topical Terms:

1017686
Biology, Cell.

The role of zinc in DNA damage and repair.
LDR:02811nam 2200265 a 45 001 929437
005 20110427
008 110427s2002 eng d
020 $a 0493663045
035 $a (UnM)AAI3051557
035 $a AAI3051557
040 $a UnM $c UnM
100 1 $a Trapp, Christine Lee. $3 1252923
245 1 0 $a The role of zinc in DNA damage and repair.
300 $a 129 p.
500 $a Source: Dissertation Abstracts International, Volume: 63-05, Section: B, page: 2318.
502 $a Thesis (Ph.D.)--University of California, Davis, 2002.
520 $a Zn is essential for the proper functioning of over 200 enzymes and plays a major role in genetic expression, cell division and growth. Poly (ADP-ribose) polymerase (PARP) is a zinc-containing enzyme that is important in cell differentiation, gene expression and DNA replication and repair. Dialysis of PARP, under a variety of conditions, indicates that a decrease in zinc content of the enzyme is associated with a decrease in activity. I hypothesized that a nutritional zinc deficiency would result in decreased PARP activity. 3T3 fibroblasts were grown in media with varying levels of zinc (0.5, 6, 15, 45 and 90 μM). The cells were grown in experimental media for 48 h and harvested during log phase growth. PARP activity was determined by measuring the incorporation of [32P] NAD into acid-insoluble acceptors. PARP protein was assessed by western blot analysis. Ku80 expression, a non-zinc DNA repair enzyme was measured as a control. PARP activity correlated positively with media zinc concentration. Cells grown in zinc deficient media (0.5μM zinc) had PARP activity 1/10th the activity of cells grown in media containing 90μM zinc. Cells grown in 90μM zinc had a similar PARP activity as cells grown in 10% FBS. Western analysis indicated decreased levels of PARP protein at the lowest levels of zinc (0.5 and 6 μM), whereas, Ku80 expression increased. A decrease in the activity of PARP could, in part, be responsible for abnormalities of gene expression, cell division and growth seen during zinc deficiency. Day 11 rat embryos, from dams fed zinc-deficient diets however, did not have significantly lower PARP activity, despite typical teratologies. These results show, that while zinc-deficiency can have a marked effect on PARP activity in isolated rapidly growing cells, this effect is not observed on a global basis in the whole embryo. The above results suggest that while reductions in DNA repair may contribute to the teratology associated with zinc deficiency it is not a major mechanism.
590 $a School code: 0029.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Health Sciences, Nutrition. $3 1017801
690 $a 0307
690 $a 0379
690 $a 0570
710 2 0 $a University of California, Davis. $3 1018682
773 0 $t Dissertation Abstracts International $g 63-05B.
790 $a 0029
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3051557