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Characterization of vascular smooth ...

Allen, Tara Jeffrey.

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Characterization of vascular smooth muscle oxidative metabolism using (13)C-isotopomer analysis of glutamate.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Characterization of vascular smooth muscle oxidative metabolism using (13)C-isotopomer analysis of glutamate./
Author:	Allen, Tara Jeffrey.
Description:	208 p.
Notes:	Source: Dissertation Abstracts International, Volume: 61-09, Section: B, page: 4501.
Contained By:	Dissertation Abstracts International61-09B.
Subject:	Biology, Animal Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9988641
ISBN:	0599954655

Characterization of vascular smooth muscle oxidative metabolism using (13)C-isotopomer analysis of glutamate.
Allen, Tara Jeffrey.

Characterization of vascular smooth muscle oxidative metabolism using (13)C-isotopomer analysis of glutamate. - 208 p.

Source: Dissertation Abstracts International, Volume: 61-09, Section: B, page: 4501.

Thesis (Ph.D.)--University of Missouri - Columbia, 2000.

Vascular smooth muscle (VSM) a primary cellular component of the arterial blood vessel wall and mediates regulation of blood pressure. The energetic fuel needed to perform this function is derived from oxidative metabolism. Although VSM produces the majority of its energy via oxidative metabolism controversy surrounds the nature of the substrates oxidized by VSM. To determine the identity of the substrates for oxidative metabolism in VSM, hog carotid arteries were incubated in the presence of variety of exogenous and endogenous substrates with subsequent analysis by <super>13</super>C-isotopomer analysis of glutamate to determine the percent contribution of these substrates to oxidative metabolism in VSM. Using the simple substrate regimes of this study we determined that exogenous substrates account for the majority (70–80%) of substrates oxidized by VSM compared to endogenous substrates (30–20%). Of the exogenous substrates examined in this study, glucose was the primary substrate oxidized accounting for &sim;40–50% of substrates oxidized in the presence of both short and long chain fatty acids and in VSM with glycogen contents spanning the range reported in vivo. Examination of fatty acid oxidation in the presence of glucose revealed that exogenous short chain fatty acids (&sim;30% of total substrates oxidized) but not long chain fatty acids (<5%) are substantial oxidative substrates for VSM. In contrast to the exogenous substrates, endogenous glycogen and lipids are minor contributors to substrate oxidation accounting for only 0–10% and 20% of substrates oxidized by VSM, respectively. In addition, regulation of substrate oxidation in VSM occurs within substrate classes (carbohydrate versus carbohydrate and lipid versus lipid) but not between substrate classes (carbohydrate versus lipids). This is supported by data demonstrating that glucose oxidation was only significantly reduced as a function of glycogen oxidation but not by addition of exogenous fatty acids. In conclusion, our data demonstrate that exogenous glucose and short chain fatty acids are the primary (70–80%) substrates oxidized by VSM in the presence of a physiological mixture of substrates, with glucose being the single largest contributor to substrate oxidation. Therefore, endogenous substrates (glycogen and lipids) are not substantial contributors to substrate oxidation in the presence of a physiological mixture of exogenous substrates.

ISBN: 0599954655Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Characterization of vascular smooth muscle oxidative metabolism using (13)C-isotopomer analysis of glutamate.
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100 1 $a Allen, Tara Jeffrey. $3 1252902
245 1 0 $a Characterization of vascular smooth muscle oxidative metabolism using (13)C-isotopomer analysis of glutamate.
300 $a 208 p.
500 $a Source: Dissertation Abstracts International, Volume: 61-09, Section: B, page: 4501.
500 $a Supervisor: Christopher D. Hardin.
502 $a Thesis (Ph.D.)--University of Missouri - Columbia, 2000.
520 $a Vascular smooth muscle (VSM) a primary cellular component of the arterial blood vessel wall and mediates regulation of blood pressure. The energetic fuel needed to perform this function is derived from oxidative metabolism. Although VSM produces the majority of its energy via oxidative metabolism controversy surrounds the nature of the substrates oxidized by VSM. To determine the identity of the substrates for oxidative metabolism in VSM, hog carotid arteries were incubated in the presence of variety of exogenous and endogenous substrates with subsequent analysis by <super>13</super>C-isotopomer analysis of glutamate to determine the percent contribution of these substrates to oxidative metabolism in VSM. Using the simple substrate regimes of this study we determined that exogenous substrates account for the majority (70–80%) of substrates oxidized by VSM compared to endogenous substrates (30–20%). Of the exogenous substrates examined in this study, glucose was the primary substrate oxidized accounting for &sim;40–50% of substrates oxidized in the presence of both short and long chain fatty acids and in VSM with glycogen contents spanning the range reported in vivo. Examination of fatty acid oxidation in the presence of glucose revealed that exogenous short chain fatty acids (&sim;30% of total substrates oxidized) but not long chain fatty acids (<5%) are substantial oxidative substrates for VSM. In contrast to the exogenous substrates, endogenous glycogen and lipids are minor contributors to substrate oxidation accounting for only 0–10% and 20% of substrates oxidized by VSM, respectively. In addition, regulation of substrate oxidation in VSM occurs within substrate classes (carbohydrate versus carbohydrate and lipid versus lipid) but not between substrate classes (carbohydrate versus lipids). This is supported by data demonstrating that glucose oxidation was only significantly reduced as a function of glycogen oxidation but not by addition of exogenous fatty acids. In conclusion, our data demonstrate that exogenous glucose and short chain fatty acids are the primary (70–80%) substrates oxidized by VSM in the presence of a physiological mixture of substrates, with glucose being the single largest contributor to substrate oxidation. Therefore, endogenous substrates (glycogen and lipids) are not substantial contributors to substrate oxidation in the presence of a physiological mixture of exogenous substrates.
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