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Chromatin remodeling in the mammalia...

Corey, Laura Lynn.

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Chromatin remodeling in the mammalian heat shock response.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Chromatin remodeling in the mammalian heat shock response./
Author:	Corey, Laura Lynn.
Description:	123 p.
Notes:	Adviser: Robert E. Kingston.
Contained By:	Dissertation Abstracts International63-04B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3051137
ISBN:	0493656456

Chromatin remodeling in the mammalian heat shock response.
Corey, Laura Lynn.

Chromatin remodeling in the mammalian heat shock response. - 123 p.

Adviser: Robert E. Kingston.

Thesis (Ph.D.)--Harvard University, 2002.

Transcriptional activators regulate gene expression by a variety of mechanisms. They stimulate transcription through interactions with the basal transcription machinery, general transcription factors and chromatin remodeling factors. The cellular stress response is a highly regulated transcriptional response in which all of these interactions are called into play.

ISBN: 0493656456Subjects--Topical Terms:

1017730
Biology, Genetics.

Chromatin remodeling in the mammalian heat shock response.
LDR:03432nam 2200313 a 45 001 927730
005 20110425
008 110425s2002 eng d
020 $a 0493656456
035 $a (UnM)AAI3051137
035 $a AAI3051137
040 $a UnM $c UnM
100 1 $a Corey, Laura Lynn. $3 1251293
245 1 0 $a Chromatin remodeling in the mammalian heat shock response.
300 $a 123 p.
500 $a Adviser: Robert E. Kingston.
500 $a Source: Dissertation Abstracts International, Volume: 63-04, Section: B, page: 1695.
502 $a Thesis (Ph.D.)--Harvard University, 2002.
520 $a Transcriptional activators regulate gene expression by a variety of mechanisms. They stimulate transcription through interactions with the basal transcription machinery, general transcription factors and chromatin remodeling factors. The cellular stress response is a highly regulated transcriptional response in which all of these interactions are called into play.
520 $a Upon exposure to a variety of stressors, the transactivator Heat Shock Factor One (HSF1) binds to the hsp70 promoter, induces release of a stable promoter-proximal paused RNA polymerase II complex, and stimulates production of full length hsp70 transcripts. These events are accompanied by transcription-independent chromatin remodeling. <italic>In vitro,</italic> mutations in acidic residues of the HSF1 activation domains impair reinitiation and targeting of the SWI/SNF chromatin remodeling complex while mutations in phenylalanine residues impair elongation on chromatin templates and abrogate SWI/SNF targeting.
520 $a The goal of the work described in this thesis is to elucidate the relationship between transcriptional elongation and chromatin remodeling during HSF1 function in vivo. To examine the phenotypes of the HSF1 mutations described above in a native chromatin context, immortalized fibroblasts from an HSF1 (−/−) mouse were used to make stable cell lines that express wild-type, acidic-mutant and phenylalanine-mutant human HSF1. In these cell lines, HSF1 is required for stress tolerance, hsp70 mRNA induction and heat-induced chromatin remodeling of the hsp70 gene. SWI/SNF is recruited to the hsp70 promoter by HSF1 following heat stress. Wild-type human HSF1 restores all of the functions of mouse HSF1. While cells expressing the acidic mutant survive heat stress as well as those expressing wild-type HSF1, they show reduced levels of hsp70 mRNA induction, heat shock-induced chromatin remodeling, and SWI/SNF recruitment. Phenylalanine-mutant hHSF1 does not confer stress tolerance, hsp70 induction, remodeling, or recruitment. However, this mutant does direct the production of high levels of abortive hsp70 transcripts following heat shock.
520 $a The ability to stimulate elongation on the hsp70 gene is critical for the heat shock response and transcriptional elongation by HSF1 is strongly correlated with its ability to direct chromatin remodeling by the SWI/SNF complex. This work supports a model of hsp70 activation in which HSF1-mediated recruitment of SWI/SNF and subsequent remodeling drive elongation through the gene.
590 $a School code: 0084.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0369
710 2 0 $a Harvard University. $3 528741
773 0 $t Dissertation Abstracts International $g 63-04B.
790 $a 0084
790 1 0 $a Kingston, Robert E., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3051137