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Quantitative analysis of hepatitis C...

Boisvert, Judith Esther.

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Quantitative analysis of hepatitis C virus in peripheral leukocytes and phenotypic analysis of liver-infiltrating lymphocytes in HCV infection.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Quantitative analysis of hepatitis C virus in peripheral leukocytes and phenotypic analysis of liver-infiltrating lymphocytes in HCV infection./
Author:	Boisvert, Judith Esther.
Description:	149 p.
Notes:	Adviser: Harry B. Greenberg.
Contained By:	Dissertation Abstracts International63-01B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3038067
ISBN:	0493516816

Quantitative analysis of hepatitis C virus in peripheral leukocytes and phenotypic analysis of liver-infiltrating lymphocytes in HCV infection.
Boisvert, Judith Esther.

Quantitative analysis of hepatitis C virus in peripheral leukocytes and phenotypic analysis of liver-infiltrating lymphocytes in HCV infection. - 149 p.

Adviser: Harry B. Greenberg.

Thesis (Ph.D.)--Stanford University, 2002.

Collectively, these results constitute a valuable contribution to the field of HCV pathogenesis and immunology.

ISBN: 0493516816Subjects--Topical Terms:

1017734
Biology, Microbiology.

Quantitative analysis of hepatitis C virus in peripheral leukocytes and phenotypic analysis of liver-infiltrating lymphocytes in HCV infection.
LDR:03408nam 2200337 a 45 001 927660
005 20110425
008 110425s2002 eng d
020 $a 0493516816
035 $a (UnM)AAI3038067
035 $a AAI3038067
040 $a UnM $c UnM
100 1 $a Boisvert, Judith Esther. $3 1251224
245 1 0 $a Quantitative analysis of hepatitis C virus in peripheral leukocytes and phenotypic analysis of liver-infiltrating lymphocytes in HCV infection.
300 $a 149 p.
500 $a Adviser: Harry B. Greenberg.
500 $a Source: Dissertation Abstracts International, Volume: 63-01, Section: B, page: 0068.
502 $a Thesis (Ph.D.)--Stanford University, 2002.
520 $a Collectively, these results constitute a valuable contribution to the field of HCV pathogenesis and immunology.
520 $a These results are significant because of their relevance not only to tissue tropism, but also to in vitro cell culture.
520 $a Hepatitis C virus (HCV) is a hepatotropic virus with a worldwide incidence estimated at 3%. It has been suggested that HCV is able to establish a productive infection in extrahepatic reservoirs, namely peripheral lymphocytes. Furthermore, while much is known about the peripheral immune response, far less is known about the intrahepatic response against HCV. This two-part thesis addresses both of these issues.
520 $a The first goal of this work was to characterize the nature of HCV's association with peripheral blood mononuclear cells. Chronic HCV patients were recruited and viral load was measured in the following purified leukocyte subpopulations: polymorphonuclear cells (PMNs), T cells, B cells and monocytes. Replication was then assayed by the specific detection of negative sense viral RNA. A preferential association of HCV with B cells was found, while T cells were virtually negative and PMNs and monocytes displayed very low levels of viral RNA. Because peripheral leukocytes are in a resting state, it was thought that cellular activation might also stimulate HCV to go from a latent or non-replicating state, to an actively replicating state. Purified peripheral B cells from chronic HCV patients were stimulated in vitro and both total viral RNA and negative-strand viral RNA were measured. It was thus determined, that under these particular conditions, HCV replication is not occurring.
520 $a The second goal of this work was to phenotypically characterize the lymphocytic infiltrate in end-stage and chronic HCV infection. Lymphocytes were isolated from liver tissue samples of HCV patients. These liver-infiltrating lymphocytes were evaluated by flow cytometry for a number of different cell surface markers, including subpopulation-defining molecules, T cell receptor, co-receptor, activation molecule and chemokine receptor expression patterns.
520 $a These results are significant because of the important role of the site-specific immune response, our emerging understanding of the role played by chemokines in such site-specific responses and our need for better understanding of what constitutes an effective immune response against HCV.
590 $a School code: 0212.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Health Sciences, Immunology. $3 1017716
690 $a 0410
690 $a 0982
710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 63-01B.
790 $a 0212
790 1 0 $a Greenberg, Harry B., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3038067