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Stress response genes in the human p...

Kim, Doyeob.

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Stress response genes in the human proximal tubules.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Stress response genes in the human proximal tubules./
Author:	Kim, Doyeob.
Description:	135 p.
Notes:	Chairs: Donald A. Sens; Joginder Nath.
Contained By:	Dissertation Abstracts International63-09B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3064594
ISBN:	0493835776

Stress response genes in the human proximal tubules.
Kim, Doyeob.

Stress response genes in the human proximal tubules. - 135 p.

Chairs: Donald A. Sens; Joginder Nath.

Thesis (Ph.D.)--West Virginia University, 2002.

The heat shock proteins (hsps) are believed to provide protection against a variety of insults such as elevated temperature, heavy metals and chemical agents. The goal of this study was to determine if the immortalized HK-2 human proximal tubule cell line could provide a model system to study the stress response of the proximal tubule cell. Heat stress, elevated temperature at 42.5°C for 1 hr, caused a marked increase only in hsp 70 mRNA and protein, but not that of hsp 27 or hsp 60 mRNA and protein. Similar results were obtained when the cells were subjected to 100 μM sodium arsenite or 53.4 μM CdCl<sub> 2</sub> for 4 hrs. These findings were in contrast to those found previously with mortal human proximal tubule (HPT) cells, where acute stress by all three stimuli elicited marked increases in hsp 27, hsp 60 and hsp 70 mRNA and protein. The basal levels of expression of hsp 27 and hsp 60 in the HK-2 cells were elevated compared to unstressed HPT cells. These results suggest that failure of HK-2 cells to increase hsp 27 and hsp 60 levels in response to stress is because of their elevated basal levels, indicating that the genetic events that resulted in the immortalization of the HK-2 cells also elicited a stress response for hsp 27 and hsp 60, but not for hsp 70. Thus, there are differences in the regulation of the stress response between the immortal HK-2 and mortal HPT cell lines, and as long as these differences are recognized, the HK-2 cell line should be a valuable adjunct to study the stress response of the proximal tubule in general and when exposed to environmental pollutants such as cadmium.

ISBN: 0493835776Subjects--Topical Terms:

1017686
Biology, Cell.

Stress response genes in the human proximal tubules.
LDR:03678nam 2200301 a 45 001 927242
005 20110425
008 110425s2002 eng d
020 $a 0493835776
035 $a (UnM)AAI3064594
035 $a AAI3064594
040 $a UnM $c UnM
100 1 $a Kim, Doyeob. $3 1250794
245 1 0 $a Stress response genes in the human proximal tubules.
300 $a 135 p.
500 $a Chairs: Donald A. Sens; Joginder Nath.
500 $a Source: Dissertation Abstracts International, Volume: 63-09, Section: B, page: 4033.
502 $a Thesis (Ph.D.)--West Virginia University, 2002.
520 $a The heat shock proteins (hsps) are believed to provide protection against a variety of insults such as elevated temperature, heavy metals and chemical agents. The goal of this study was to determine if the immortalized HK-2 human proximal tubule cell line could provide a model system to study the stress response of the proximal tubule cell. Heat stress, elevated temperature at 42.5°C for 1 hr, caused a marked increase only in hsp 70 mRNA and protein, but not that of hsp 27 or hsp 60 mRNA and protein. Similar results were obtained when the cells were subjected to 100 μM sodium arsenite or 53.4 μM CdCl<sub> 2</sub> for 4 hrs. These findings were in contrast to those found previously with mortal human proximal tubule (HPT) cells, where acute stress by all three stimuli elicited marked increases in hsp 27, hsp 60 and hsp 70 mRNA and protein. The basal levels of expression of hsp 27 and hsp 60 in the HK-2 cells were elevated compared to unstressed HPT cells. These results suggest that failure of HK-2 cells to increase hsp 27 and hsp 60 levels in response to stress is because of their elevated basal levels, indicating that the genetic events that resulted in the immortalization of the HK-2 cells also elicited a stress response for hsp 27 and hsp 60, but not for hsp 70. Thus, there are differences in the regulation of the stress response between the immortal HK-2 and mortal HPT cell lines, and as long as these differences are recognized, the HK-2 cell line should be a valuable adjunct to study the stress response of the proximal tubule in general and when exposed to environmental pollutants such as cadmium.
520 $a The metallothoneins (MT) are a family of cysteine-rich, low molecular weight (6 kD), intracellular proteins that bind transitional metals. The goal of this study was to further characterize the basal expression of MT-3 in the <italic>in situ</italic> human kidney, in mortal human proximal tubule (HPT) cell cultures and the immortalized proximal tubular cell line, HK-2. MT-3 mRNA was detected in the proximal tubule of the in situ kidney with relative expression in excess to that of the ∃-actin housekeeping gene. Human proximal tubule cells also expressed both MT-3 mRNA and protein, and were able to form domes. Exposure of HPT cells to Cd<super>+2</super> resulted in a transient increase in MT-3 mRNA and protein. In contrast, the HK-2 cell line did not express MT-3 and did not form domes. Stable transfection of the HK-2 cell line with the pcDNA3.1/Hygro(+) vector containing the MT-3 gene restored MT-3 expression and dome formation to the HK-2 cells. This result demonstrated that MT-3 is involved in the transport function of a human renal cell line that retains properties of the proximal tubule.
590 $a School code: 0256.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Genetics. $3 1017730
690 $a 0369
690 $a 0379
710 2 0 $a West Virginia University. $3 1017532
773 0 $t Dissertation Abstracts International $g 63-09B.
790 $a 0256
790 1 0 $a Nath, Joginder, $e advisor
790 1 0 $a Sens, Donald A., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3064594