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Synthesis and characterization of si...

Dytioco, Rovelyn Tapec.

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Synthesis and characterization of silica-based nanoparticles for bioanalytical applications.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Synthesis and characterization of silica-based nanoparticles for bioanalytical applications./
Author:	Dytioco, Rovelyn Tapec.
Description:	146 p.
Notes:	Chair: Weihong Tan.
Contained By:	Dissertation Abstracts International63-09B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3065929
ISBN:	0493849912

Synthesis and characterization of silica-based nanoparticles for bioanalytical applications.
Dytioco, Rovelyn Tapec.

Synthesis and characterization of silica-based nanoparticles for bioanalytical applications. - 146 p.

Chair: Weihong Tan.

Thesis (Ph.D.)--University of Florida, 2002.

Novel silica-based nanoparticles that may improve and create an impact in bioanalyses have been developed. Highly luminescent dye-doped silica nanoparticles with excellent photostability were synthesized and used as probes or biomarkers for various biochemical processes. Ultra-small magnetic silica nanoparticles were also made and used as carriers in separation processes involving biomolecules. Due to the silica matrix, both types of nanoparticles can be easily conjugated to biomolecules for further use in bioanalyses.

ISBN: 0493849912Subjects--Topical Terms:

586156
Chemistry, Analytical.

Synthesis and characterization of silica-based nanoparticles for bioanalytical applications.
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020 $a 0493849912
035 $a (UnM)AAI3065929
035 $a AAI3065929
040 $a UnM $c UnM
100 1 $a Dytioco, Rovelyn Tapec. $3 1250490
245 1 0 $a Synthesis and characterization of silica-based nanoparticles for bioanalytical applications.
300 $a 146 p.
500 $a Chair: Weihong Tan.
500 $a Source: Dissertation Abstracts International, Volume: 63-09, Section: B, page: 4152.
502 $a Thesis (Ph.D.)--University of Florida, 2002.
520 $a Novel silica-based nanoparticles that may improve and create an impact in bioanalyses have been developed. Highly luminescent dye-doped silica nanoparticles with excellent photostability were synthesized and used as probes or biomarkers for various biochemical processes. Ultra-small magnetic silica nanoparticles were also made and used as carriers in separation processes involving biomolecules. Due to the silica matrix, both types of nanoparticles can be easily conjugated to biomolecules for further use in bioanalyses.
520 $a RuBpy dye-doped silica and silica-coated magnetic nanoparticles were synthesized in reverse microemulsion. This size-selective microemulsion system allowed the formation of monodisperse nanoparticles with diameters from 2–3 nm to 70 nm. Rhodamine 6G dye-doped nanoparticles were prepared using a modified Stöber method. The procedure yielded 100-nm and bigger particles.
520 $a The dye-doped nanoparticles were characterized not only for their size but also for their optical properties. These particles have excellent photostability due to the shielding effect provided by the silica matrix. Composition analysis done on the nanoparticles showed that each particle contains thousands of dye molecules, which can be a basis for signal amplification. The nanoparticles were used in a DNA assay and detection down to 100 fM was achieved. In addition, signal amplification was observed when the intensities from assays using dye-doped nanoparticle label and organic fluorophore label were compared.
520 $a Silica-coated magnetic nanoparticles were characterized according to their size and magnetic susceptibility. The particles obtained were in the nanometer range and were superparamagnetic. These nanoparticles were used in the collection of trace amounts of oligonucleotides from a complex matrix. With the silica coating, the magnetic nanoparticles were bound to molecular beacons, which acted as probes and capturing elements for the target oligonucleotide. Collection efficiency using these magnetic nanoparticles conjugated with molecular beacons was as high as 98%.
520 $a Both the synthesis routes developed and the assay results may be used in future studies to either further improve the nanoparticles or optimize the nanoparticle-based assays. This work is one indication that nanotechnology can benefit, if not revolutionize, biotechnology.
590 $a School code: 0070.
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0486
710 2 0 $a University of Florida. $3 718949
773 0 $t Dissertation Abstracts International $g 63-09B.
790 $a 0070
790 1 0 $a Tan, Weihong, $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3065929