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Characterization of the metal bindin...

Bowling Green State University.

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Characterization of the metal binding properties of de novo designed coiled coil metalloproteins.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of the metal binding properties of de novo designed coiled coil metalloproteins./
Author:	Zhu, Xianchun.
Description:	176 p.
Notes:	Adviser: Michael Y. Ogawa.
Contained By:	Dissertation Abstracts International70-03B.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3349460
ISBN:	9781109063677

Characterization of the metal binding properties of de novo designed coiled coil metalloproteins.
Zhu, Xianchun.

Characterization of the metal binding properties of de novo designed coiled coil metalloproteins. - 176 p.

Adviser: Michael Y. Ogawa.

Thesis (Ph.D.)--Bowling Green State University, 2009.

The dissertation aims to elucidate three effects on the metal binding properties of de novo designed coiled coil metalloproteins, which are the stability of the peptide, the placement of the metal binding motif along the peptide sequence, and the variation of the metal binding motif.

ISBN: 9781109063677Subjects--Topical Terms:

1019105
Biophysics, General.

Characterization of the metal binding properties of de novo designed coiled coil metalloproteins.
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020 $a 9781109063677
035 $a (UMI)AAI3349460
035 $a AAI3349460
040 $a UMI $c UMI
100 1 $a Zhu, Xianchun. $3 1064539
245 1 0 $a Characterization of the metal binding properties of de novo designed coiled coil metalloproteins.
300 $a 176 p.
500 $a Adviser: Michael Y. Ogawa.
500 $a Source: Dissertation Abstracts International, Volume: 70-03, Section: B, page: 1697.
502 $a Thesis (Ph.D.)--Bowling Green State University, 2009.
520 $a The dissertation aims to elucidate three effects on the metal binding properties of de novo designed coiled coil metalloproteins, which are the stability of the peptide, the placement of the metal binding motif along the peptide sequence, and the variation of the metal binding motif.
520 $a Starting from a previously designed peptide C16C19-GGY, a more stable peptide was designed. The new peptide is AQC16C19-GGY. Its Cd(II) complex is a trimer containing 4 Cd(II). In contrast, the Cd(II) complex of C16C19-GGY is a dimer with one Cd(II). The process of the incorporation of Cd(II) into AQC16C19-GGY is shown to be a fast bond formation process followed by a slow conformation change. Therefore, the stability of the peptide affects the metal binding properties.
520 $a The effect of the placement of the metal binding motif along the peptide sequence on the metal binding properties was demonstrated by a series of peptides derived from the parent peptide: K(IEALEGK)4GGY. The peptides are C2C5-GGY, C9C12-GGY, C16C19-GGY (the previously studied one), and C23C26-GGY. The Cu(I) binding properties of these peptides were studied and compared. C2C5-GGY and C9C12-GGY have similar metal binding properties. C23C26-GGY has different metal binding properties from those of C2C5-GGY and C9C12-GGY, but its metal binding properties are similar to those of C16C19-GGY. The placement of the metal binding motif along the peptide sequence also plays a role in determining the oligomerization states of the metalloproteins.
520 $a In the last chapter, the C-X-X-C metal binding motif was changed to C-X-X-H to study the effect of the first coordination sphere on the metal binding properties. It is seen that the first coordination sphere has an effect on the metal binding properties. Interestingly, one of the obtained metalloproteins shows pH-dependent luminescence properties.
590 $a School code: 0018.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Chemistry, Physical. $3 560527
690 $a 0494
690 $a 0786
710 2 0 $a Bowling Green State University. $3 676285
773 0 $t Dissertation Abstracts International $g 70-03B.
790 $a 0018
790 1 0 $a Ogawa, Michael Y., $e advisor
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3349460