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Investigation of bile stress toleran...

Michigan State University.

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Investigation of bile stress tolerance and immunomodulatory properties of probiotic Lactobacillus reuteri.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Investigation of bile stress tolerance and immunomodulatory properties of probiotic Lactobacillus reuteri./
Author:	Whitehead, Kristi James.
Description:	197 p.
Notes:	Adviser: Robert A. Britton.
Contained By:	Dissertation Abstracts International70-07B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3364764
ISBN:	9781109245134

Investigation of bile stress tolerance and immunomodulatory properties of probiotic Lactobacillus reuteri.
Whitehead, Kristi James.

Investigation of bile stress tolerance and immunomodulatory properties of probiotic Lactobacillus reuteri. - 197 p.

Adviser: Robert A. Britton.

Thesis (Ph.D.)--Michigan State University, 2009.

This thesis focuses on characterization of different aspects of L. reuteri physiology that may contribute to the probiotic potential of the species. This research includes an investigation of the bile stress response of L. reuteri ATCC 55730 (Chapter 2), analysis of the bile salt hydrolase activity of L. reuteri ATCC PTA 6475 (Chapter 3), and the investigation of the potential immunomodulatory rote of lactobacillic acid, a cyclopropyl fatty acid that is specific to certain strains of L. reuteri at particular stages of growth (Chapter 4).

ISBN: 9781109245134Subjects--Topical Terms:

1017734
Biology, Microbiology.

Investigation of bile stress tolerance and immunomodulatory properties of probiotic Lactobacillus reuteri.
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020 $a 9781109245134
035 $a (UMI)AAI3364764
035 $a AAI3364764
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100 1 $a Whitehead, Kristi James. $3 1059000
245 1 0 $a Investigation of bile stress tolerance and immunomodulatory properties of probiotic Lactobacillus reuteri.
300 $a 197 p.
500 $a Adviser: Robert A. Britton.
500 $a Source: Dissertation Abstracts International, Volume: 70-07, Section: B, page: .
502 $a Thesis (Ph.D.)--Michigan State University, 2009.
520 $a This thesis focuses on characterization of different aspects of L. reuteri physiology that may contribute to the probiotic potential of the species. This research includes an investigation of the bile stress response of L. reuteri ATCC 55730 (Chapter 2), analysis of the bile salt hydrolase activity of L. reuteri ATCC PTA 6475 (Chapter 3), and the investigation of the potential immunomodulatory rote of lactobacillic acid, a cyclopropyl fatty acid that is specific to certain strains of L. reuteri at particular stages of growth (Chapter 4).
520 $a The research contained within Chapter 2 includes an investigation of the physiological response of L. reuteri ATCC 55730 to the bile stress that would be encountered in the host small intestine, including an analysis of genes that are important for survival and growth in the presence of bile. Three genes were identified as being important for the survival of L. reuteri ATCC 55730 in bile: a putative esterase, a clp chaperone, and a gene of unknown function that is conserved within different L. reuteri isolates. One gene, encoding a multidrug resistance protein in the major facilitator superfamily, was found to contribute to the ability of the strain to grow in the presence of bile.
520 $a Chapter 3 examines the ability of L. reuteri ATCC PTA 6475 to deconjugate bile acids. There is a strong correlation between strains isolated from the gastrointestinal tract and the ability to deconjugate bile acids, thus it is often suggested that bile salt hydrolase activity confers a selective advantage for colonization of the gastrointestinal tract. L. reuteri ATCC 55730, the strain investigated in the microarray and mutational analysis of the bile stress response, exhibits a very low level of bile salt hydrolase activity. L. reuteri ATCC PTA 6475, on the other hand, exhibits strong bile salt hydrolase activity against all six major human bile acids. The research contained in Chapter 3 suggests that this activity confers a growth advantage in the presence of bile acids at slightly acidic pH levels.
520 $a Several strains of L. reuteri have been suggested to be able to modulate the host immune response through the production of compounds that inhibit production of the pro-inflammatory cytokine, TNF. The research in Chapter 4 identifies a possible novel immunomodulatory compound, lactobacillic acid, which may be responsible for the observed suppression. Production of this compound was demonstrated to be specific to L. reuteri strains with the TNF suppressive activity, and importantly, the appearance of lactobacillic acid in cells isolated from early stationary and late stationary phase cultures correlates with the growth stages where the immunomodulatory activity is observed. Current research is focused on identifying the role of lactobacillic acid in TNF suppression.
590 $a School code: 0128.
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0410
710 2 $a Michigan State University. $3 676168
773 0 $t Dissertation Abstracts International $g 70-07B.
790 $a 0128
790 1 0 $a Britton, Robert A., $e advisor
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3364764