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Sequence diversity and expression of...

University of Puerto Rico, Mayaguez (Puerto Rico).

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Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments./
Author:	Rodriguez-Castano, Gina Paola.
Description:	182 p.
Notes:	Adviser: Arturo A. Massol-Deya.
Contained By:	Masters Abstracts International47-02.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1458836
ISBN:	9780549798422

Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments.
Rodriguez-Castano, Gina Paola.

Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments. - 182 p.

Adviser: Arturo A. Massol-Deya.

Thesis (M.S.)--University of Puerto Rico, Mayaguez (Puerto Rico), 2009.

Nitrous oxide (N2O) is a greenhouse gas produced in different microbial processes. Nitrous oxide reductases (N2ORs) are enzymes that catalyze N2O transformation to N2. Understanding the diversity of nitrous oxide reductases genes (nosZ ) is crucial to predict and manage emissions. This study developed oligonucleotides for amplifying a broader range of nosZ genes to assess their diversity in different environments. Sequences obtained were different from traditional nosZ sequences (62% amino acid similarity). Neither of the clone sequences fell into the traditional NosZ phylogenetic clades, but grouped with nontraditional NosZ sequences from Anaeromyxobacter spp., Magnetospirillum spp., Desulfitobacterium hafniense, and Dechloromonas aromatica. The detection of nosZ genes in the environment was achieved by ISRT-PCR/FISH using an internal fluorescently-labeled NosZ943 probe. We demonstrate the existence of many variants of nosZ gene. These variants could represent a high functional diversity for reducing N2O.

ISBN: 9780549798422Subjects--Topical Terms:

1017734
Biology, Microbiology.

Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments.
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020 $a 9780549798422
035 $a (UMI)AAI1458836
035 $a AAI1458836
040 $a UMI $c UMI
100 1 $a Rodriguez-Castano, Gina Paola. $3 1036095
245 1 0 $a Sequence diversity and expression of novel bacterial nitrous oxide reductase (nosZ) genes in tropical environments.
300 $a 182 p.
500 $a Adviser: Arturo A. Massol-Deya.
500 $a Source: Masters Abstracts International, Volume: 47-02, page: 0886.
502 $a Thesis (M.S.)--University of Puerto Rico, Mayaguez (Puerto Rico), 2009.
520 $a Nitrous oxide (N2O) is a greenhouse gas produced in different microbial processes. Nitrous oxide reductases (N2ORs) are enzymes that catalyze N2O transformation to N2. Understanding the diversity of nitrous oxide reductases genes (nosZ ) is crucial to predict and manage emissions. This study developed oligonucleotides for amplifying a broader range of nosZ genes to assess their diversity in different environments. Sequences obtained were different from traditional nosZ sequences (62% amino acid similarity). Neither of the clone sequences fell into the traditional NosZ phylogenetic clades, but grouped with nontraditional NosZ sequences from Anaeromyxobacter spp., Magnetospirillum spp., Desulfitobacterium hafniense, and Dechloromonas aromatica. The detection of nosZ genes in the environment was achieved by ISRT-PCR/FISH using an internal fluorescently-labeled NosZ943 probe. We demonstrate the existence of many variants of nosZ gene. These variants could represent a high functional diversity for reducing N2O.
590 $a School code: 0553.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0410
710 2 $a University of Puerto Rico, Mayaguez (Puerto Rico). $3 1017811
773 0 $t Masters Abstracts International $g 47-02.
790 $a 0553
790 1 0 $a Massol-Deya, Arturo A., $e advisor
791 $a M.S.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1458836