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Microdevices for pathogen detection.

University of California, Berkeley with the University of California, San Francisco.

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Microdevices for pathogen detection.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Microdevices for pathogen detection./
Author:	Beyor, Nathaniel Winslow.
Description:	107 p.
Notes:	Adviser: Richard A. Mathies.
Contained By:	Dissertation Abstracts International69-10B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3331535
ISBN:	9780549833833

Microdevices for pathogen detection.
Beyor, Nathaniel Winslow.

Microdevices for pathogen detection. - 107 p.

Adviser: Richard A. Mathies.

Thesis (Ph.D.)--University of California, Berkeley with the University of California, San Francisco, 2008.

Microfluidic technology for pathogen detection from a complex input sample has been developed. The technology uses a fluidized bed of immunomagnetic beads to concentrate and purify target cells from a dilute sample. This capture technology is integrated with analysis using microfluidic elements such as an on-chip pump, a cell capture structure, a polymerase chain reaction (PCR) chamber, and a capillary electrophoresis (CE) separation channel.

ISBN: 9780549833833Subjects--Topical Terms:

586156
Chemistry, Analytical.

Microdevices for pathogen detection.
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020 $a 9780549833833
035 $a (UMI)AAI3331535
035 $a AAI3331535
040 $a UMI $c UMI
100 1 $a Beyor, Nathaniel Winslow. $3 1036062
245 1 0 $a Microdevices for pathogen detection.
300 $a 107 p.
500 $a Adviser: Richard A. Mathies.
500 $a Source: Dissertation Abstracts International, Volume: 69-10, Section: B, page: .
502 $a Thesis (Ph.D.)--University of California, Berkeley with the University of California, San Francisco, 2008.
520 $a Microfluidic technology for pathogen detection from a complex input sample has been developed. The technology uses a fluidized bed of immunomagnetic beads to concentrate and purify target cells from a dilute sample. This capture technology is integrated with analysis using microfluidic elements such as an on-chip pump, a cell capture structure, a polymerase chain reaction (PCR) chamber, and a capillary electrophoresis (CE) separation channel.
520 $a In Chapter 1, I discuss the need for rapid and sensitive pathogen detection to slow the growing threat of infectious disease. The advantages of lab-on-a-chip systems for this application are highlighted, including their size, speed, sensitivity, automation, and portability. Six different microfluidic technologies for pathogen detection are described in detail and compared.
520 $a Chapter 2 presents a chronological description of my work developing a microfluidic cell capture device. Using a fluidized bed of immunomagnetic beads, I tested multiple channel designs including a recirculating system, which captured S. aureus at 17.6% efficiency. Due to this device's limited interrogation volume, I developed the pumping 'flutter' step technique, which allows sensitive detection from relatively large sample volumes. I present data showing that a straight channel device using this technique captures E. coli K12 at 9.6% efficiency. Chapter 3 details the variables explored in optimizing the capture structure and process. With E. coli K12 as the pathogen target, we demonstrate capture efficiencies as high as 70% and a sensitivity of 2 cfu/muL.
520 $a I integrated the optimized capture structure with a microfluidic PCR-CE system, described in Chapter 4. Strain-specific beads and PCR primers were used to capture and detect both E. coli K12 and E. coli O157. I achieved a sensitivity of 0.2 cfu/muL and successfully detected O157 in a 1000-fold higher background of K12.
520 $a I conclude by discussing how the integrated cell capture PCR-CE device measures up and how it could be improved. I present a design for an arrayed system including an extended capture structure for multiplex capture and eight PCR-CE systems for multiplex analysis. This system, which is a direct extension of the integrated device I have developed, is a significant step towards a portable, automated lab-on-a-chip system for sensitive pathogen detection.
590 $a School code: 1066.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Engineering, Biomedical. $3 1017684
690 $a 0486
690 $a 0541
710 2 $a University of California, Berkeley with the University of California, San Francisco. $3 1025062
773 0 $t Dissertation Abstracts International $g 69-10B.
790 $a 1066
790 1 0 $a Mathies, Richard A., $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3331535